Phân tích hóa học và tiềm năng chống viêm, giảm đau, hạ sốt in vitro và in vivo của Sesuvium sesuvioides (Fenzl) Verdc. (Aizoaceae)

Inflammopharmacology - Tập 29 - Trang 789-800 - 2021
M. Sajid-ur-Rehman1,2, Saiqa Ishtiaq1, Mohsin Abbas Khan3, Meshal Alshamrani4, Muhammad Younus2, Ghazala Shaheen5, Muhammad Abdullah6, Ghulam Sarwar7, Muhammad Sohaib Khan8, Faraza Javed8
1Department of Pharmacognosy, Punjab University College of Pharmacy, University of the Punjab, Lahore, Pakistan
2Department of Pharmacognosy, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
4Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan, Saudi Arabia
5Department of Eastern Medicine, University College of Conventional Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
6Cholistan Institute of Desert Studies, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
7Department of Botany, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
8Department of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan

Tóm tắt

Sesuvium sesuvioides (Fenzl) Verdc được sử dụng truyền thống trong việc điều trị các bệnh viêm nhiễm như viêm khớp và gout. Mục tiêu của nghiên cứu hiện tại là đánh giá khả năng chống viêm, giảm đau và hạ sốt của chiết xuất methanol từ Sesuvium sesuvioides (SsCr) để chứng minh về mặt khoa học việc sử dụng của nó trong các bệnh viêm và kiểm tra khả năng chống oxy hóa tổng thể của nó bằng nhiều phương pháp và các hợp chất thực vật qua GC–MS. Các nghiên cứu hóa học sơ bộ cho thấy sự hiện diện của phenol, flavonoid, glycosid, coumarin, terpenoid, saponin, chất béo và carbohydrate trong chiết xuất thô. Hàm lượng phenolic tổng cộng (27.31 ± 0.28 mg GAE/g) và hàm lượng flavonoid tổng cộng (3.58 ± 0.12 mgRE/g) được ghi nhận. Khả năng chống oxy hóa của SsCr cho thấy kết quả DPPH, ABTS, CUPRAC, FRAP, PBD và chelat kim loại đáng kể. Phân tích GC–MS đã hiển thị các thành phần thực vật có tiềm năng chống viêm như 2-methoxy-4-vinylphenol, vanillin, umbelliferone, methyl ferulate, palmitoleic acid, methyl palmitate và phytol. SsCr đã cho thấy sự ổn định màng HRBC đáng chú ý với mức ức chế tối đa sự tan máu tế bào (47.79%). Trong thí nghiệm gây phù chân sau bằng carrageenan, kết quả cho thấy tác dụng chống viêm theo liều lượng. SsCr đã thể hiện hoạt động giảm đau đáng kể (p < 0.05) trong các thử nghiệm tấm nóng và gập đuôi, cũng như cho thấy sự ức chế đáng kể về độ trễ đau khi đối phó với giảm đau do formalin ở giai đoạn 1 và 2. SsCr giảm chứng quặn thắt do axit axetic ở các liều khác nhau (250, 500 và 750 mg). Kết quả của hoạt động hạ sốt của chiết xuất SsCr có ý nghĩa ở các liều 500 và 750 mg. Các kết quả từ những nghiên cứu thực nghiệm in vitro và in vivo đã xác nhận tiềm năng chống viêm, giảm đau và hạ sốt của Sesuvium sesuvioides và hỗ trợ cho các ứng dụng dân gian của cây thuốc này.

Từ khóa


Tài liệu tham khảo

Arulselvan P, Fard MT, Tan WS, Gothai S, Fakurazi S, Norhaizan ME, Kumar SS (2016) Role of antioxidants and natural products in inflammation. OXID MED CELL LONGEV 2016:1–15. Bhatia M, Zemans RL, Jeyaseelan S (2012) Role of chemokines in the pathogenesis of acute lung injury. Am J Respir Cell Mol Biol 46:566–572 Calixto JB, Beirith A, Ferreira J, Santos AR, Filho VC, Yunes RA (2000) Naturally occurring antinociceptive substances from plants. Phytoth Res Internat J Devoted Pharmacol Toxicol Evaluation Nat Prod Derivat 14:401–418 Catherine RE (2004) Flavonoids and isoflavones: absorption, metabolism, and bioactivity. Free Radical Biol Med 7:827–828 Chang CW, Chang WT, Liao JC, Chiu YJ, Hsieh MT, Peng WH, Lin YC (2012) Analgesic and anti-inflammatory activities of methanol extract of cissus repens in mice. Evid. Based Complementary Altern. Med 2012:1–10 Chowdhury A, Azam S, Jainul MA, Faruq KO, Islam A (2014) Antibacterial activities and in vitro anti-inflammatory (Membrane stability) properties of methanolic extract of Gardenia coronaria leaves. Internat J Microbiol 2014:1–5 Demerdash E (2011) Anti-inflammatory and antifibrotic effects of methyl palmitate. Toxicol Appl Pharmacol 254:238–244 Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE (2007) Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1β generation. Clin Exp Immunol 147:227–235 Gilani AH, Jabeen Q, Khan A-U, Shah AJ (2008) Gut modulatory, blood pressure lowering, diuretic and sedative activities of cardamom. J Ethnopharmacol 115:463–472 Gilligan JP, Lovato SJ, Erion MD, Jeng AY (1994) Modulation of carrageenan-induced hind paw edema by substance P. Inflammation 18:285–292 Grochowski DM, Uysal S, Aktumsek A, Granica S, Zengin G, Ceylan R, Locatelli M, Tomczyk M (2017) In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca. Phytochem Lett 20:365–372 Hegde S, Jayaraj M, Bhandarkar A (2015) Pharmacognostic studies and preliminary phytochemical pnalysis of cold and hot Extracts of leaf of Tinospora malabarica Miers-An Important Medicinal Plant. Int J Pharm Sci Rev Res 34:19–25 Javed F, Jabeen Q, Aslam N, Awan AM (2020) Pharmacological evaluation of analgesic, anti-inflammatory and antipyretic activities of ethanolic extract of Indigofera argentea burm F. J Ethnopharmacol 256:112966 Jeong JB, Hong SC, Jeong HJ, Koo JS (2011) Anti-inflammatory effect of 2-methoxy-4-vinylphenol via the suppression of NF-κB and MAPK activation, and acetylation of histone H3. Arch Pharmacal Res 34:2109–2116 Jouyban A, Rahimpour E, Acree W (2019) Further analysis on solubility measurement and thermodynamic modeling of benzoic acid in monosolvents and binary mixtures. Pharmaceutical Sci 25:165–170 Lekouaghet A, Boutefnouchet A, Bensuici C, Gali L, Ghenaiet K, Tichati L (2020) In vitro evaluation of antioxidant and anti-inflammatory activities of the hydroalcoholic extract and its fractions from Leuzea conifera L. roots. S Afr J Bot 132:103–107 Leon LR (2002) Invited review: cytokine regulation of fever: studies using gene knockout mice. J Appl Physiol 92:2648–2655 Malvar DDC, Aguiar FA, Vaz ADL, Assis DC, de Melo MC, Jabor VA, Kalapothakis E, Ferreira SH, Clososki GC, de Souza GE (2014) Dipyrone metabolite 4-MAA induces hypothermia and inhibits PGE 2-dependent and-independent fever while 4-AA only blocks PGE 2-dependent fever. Br J Pharmacol 171:3666–3679 Mansouri MT, Hemmati AA, Naghizadeh B, Mard SA, Rezaie A, Ghorbanzadeh B (2015) A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats. Indian J Pharmacol 47:292 Mantawy EM, Tadros MG, Awad AS, Hassan DA, El-Demerdash E (2012) Insights antifibrotic mechanism of methyl palmitate: impact on nuclear factor kappa B and proinflammatory cytokines. Toxicol Appl Pharmacol 258:134–144 Mazimba O (2017) Umbelliferone: sources, chemistry and bioactivities review. Bull Faculty Pharm Cairo Univ 55:223–232 Miller SB (2006) Prostaglandins in health and disease: an overview Seminars in arthritis and rheumatism. Elsevier Moncada S, Ferreira S, Vane J (1973) Prostaglandins, aspirin-like drugs and the oedema of inflammation. Nature 246:217–219 Niazi J, Kaur N, Sachdeva RK, Bansal Y, Gupta V (2014) Anti-inflammatory and antinociceptive activity of vanillin. Drug Dev Ther 5:145 Oyekachukwu A, Elijah J, Eshu O, Nwodo O (2017) Anti-inflammatory effects of the chloroform extract of Annona muricata leaves on phospholipase A2 and prostaglandin synthase activities. Transl Biomed 8:137 Palu A, West B, Jensen C, Su C, Zhou B-N, Story S (2004) Morinda citrifolia as a 5-Lipoxygenase inhibitor. Google Patents Paramita S, Kosala K, Dzulkifli D, Saputri DI, Wijayanti E (2017) Anti-inflammatory activities of ethnomedicinal plants from Dayak Abai in North Kalimantan, Indonesia. Biodiv J Biol Div 18:1556–1561 Parvin MS, Das N, Jahan N, Akhter MA, Nahar L, Islam ME (2015) Evaluation of in vitro anti-inflammatory and antibacterial potential of Crescentia cujete leaves and stem bark. BMC Res Notes 8:412 Peerzada S, Khan MT, Akhtar MF, Saleem A, Hamid I, Akhtar B, Ali S, Ahmed S, Raza M (2020) Phytochemical, anti-inflammatory, anti-nociceptive and cytotoxic basis for the use of Haloxylon stocksii. Pak J Pharm Sci 33:887–894 Perianayagam JB, Sharma S, Joseph A, Christina A (2004) Evaluation of anti-pyretic and analgesic activity of Emblica officinalis Gaertn. J Ethnopharmacol 95:83–85 Phuong NTM, Cuong TT, Quang DN (2014) Anti-inflammatory activity of methyl ferulate isolated from Stemona tuberosa Lour. Asian Pac J Trop Med 7:S327–S331 Rahmatullah M, Hossain M, Mahmud A, Sultana N, Mizanur S, Mohammad R, Islam R, Khatoon M, Jahan S, Islam F (2013) Antihyperglycemic and antinociceptive activity evaluation of ‘khoyer’prepared from boiling the wood of Acacia catechu in water. Afr J Tradit Complement Altern Med 10:1–5 Rehman F, Hussain T, Abdullah M, Ashraf I, Ch KM, Rafay M, Bibi I (2015) Ethnobotanical survey. Prof Med J 22:1367–1372 Saleem TM, Basha SD, Mahesh G, Rani PS, Kumar NS, Chetty CM (2011) Analgesic, anti-pyretic and anti-inflammatory activity of dietary sesame oil in experimental animal models. Pharmacologia 2:172–177 Saleem M, Khalid I, Akhtar MF, Saleem A, Rasul A, Hamid I, Shah MA, Basheer E, Javaid Z, Sohail K (2020) HPLC analysis and pharmacological study of Quercus dilatata Lindle. ex Royle aqueous methanolic extract in Sprague Dawley rats. Pak J Pharm Sci 33:1327–1332 Santos CCDMP, Salvadori MS, Mota VG, Costa LM, De Almeida AAC, De Oliveira GAL, Costa JP, De Sousa DP, De Freitas RM, De Almeida RN (2013) Antinociceptive and antioxidant activities of phytol in vivo and in vitro models. Neurosci J 2013:1–9 Shahidi F, Janitha P, Wanasundara P (1992) Phenolic antioxidants. Crit Rev Food Sci Nutr 32:67–103 Shi J, Fan J, Su Q, Yang Z (2019) Cytokines and abnormal glucose and lipid metabolism. Front Endocrinol 10:703 Silva RO, Sousa FBM, Damasceno SR, Carvalho NS, Silva VG, Oliveira FRM, Sousa DP, Aragão KS, Barbosa AL, Freitas RM (2014) Phytol, a diterpene alcohol, inhibits the inflammatory response by reducing cytokine production and oxidative stress. Fundam Clin Pharmacol 28:455–464 Sim M-O, Lee H-I, Ham JR, Seo K-I, Kim M-J, Lee M-K (2015) Anti-inflammatory and antioxidant effects of umbelliferone in chronic alcohol-fed rats. Nurs Res Pract 9:364–369 Slinkard K, Singleton VL (1977) Total phenol analysis: automation and comparison with manual methods. Am J Enol Vitic 28:49–55 Sobeh M, Rezq S, Cheurfa M, Abdelfattah MA, Rashied RM, El-Shazly AM, Yasri A, Wink M, Mahmoud MF (2020) Thymus algeriensis and Thymus fontanesii: Chemical composition, in vivo antiinflammatory, pain killing and antipyretic activities: a comprehensive comparison. Biomolecules 10:599 Souza D, Oliveira C, Valenzuela CA, Baker EJ, Miles EA, Rosa Neto JC, Calder PC (2018) Palmitoleic acid has stronger anti-inflammatory potential in human endothelial cells compared to oleic and palmitic acids. Mole Nutrit Food Res 62:1800322 Sridevi G, Prema S, Sekar S, Sembulingam K (2014) Phytochemical analysis of Pergularia daemia for its bioactive components through gas chromatographic mass spectrometry (GCMS). IOSR J Pharm 4:41–46 Strober W, Fuss I, Mannon P (2007) The fundamental basis of inflammatory bowel disease. J Clin Investig 117:514–521 Sudjarwo SA (2005) The potency of piperine as antiinflammatory and analgesic in rats and mice. Folia Med Indonesiana 41:190–194 Sujarwo W, Keim AP (2019) Spondias pinnata (L.f.) Kurz.(Anacardiaceae): profiles and applications to diabetes bioactive food as dietary interventions for diabetes. Elsevier Thakre R, Harne K, Tekade P, Parve S (2020) Role of Ajan Vruksha/Khandu Chakka plant (Ehretia laevis roxb.) in COVID-19 pandemic. Internat J Res Pharma Sci 11:224–233 Wariss HM, Ahmad S, Anjum S, Alam K (2014) Ethnobotanical studies of dicotyledonous plants of Lal Suhanra national park, Bahawalpur, Pakistan. Internat J Sci Res 3:2452–2460 Zengin G, Uysal S, Ceylan R, Aktumsek A (2015) Phenolic constituent, antioxidative and tyrosinase inhibitory activity of Ornithogalum narbonense L. from Turkey: a phytochemical study. Ind Crops Prod 70:1–6 Zengin G, Nithiyanantham S, Locatelli M, Ceylan R, Uysal S, Aktumsek A, Selvi PK, Maskovic P (2016) Screening of in vitro antioxidant and enzyme inhibitory activities of different extracts from two uninvestigated wild plants: centranthus longiflorus subsp. longiflorus and Cerinthe minor subsp. auriculata. Eur J Integrative Med 8:286–292