Physical and Functional Homology between Ribosomal Protein S1 and Interference Factor i

FEBS Journal - Tập 45 Số 1 - Trang 109-117 - 1974
Hiroshi Inouye1, Yaakov Pollack1, Jean Pêtre1
1Biochemistry Department, Weizmann Institute of Science P.O. Box 26, Rehovot, Israel

Tóm tắt

The homology between the 30‐S ribosomal protein S1 and interference factor i has been investigated.The two proteins are indistinguishable by gel electrophoresis under various conditions. Similar peptides are obtained by cleavage with cyanogen bromide. After labelling with N‐ethylmaleimide, enzymatic digestion yields homologous labelled peptides. This homology is confirmed by immunological data.Characteristic activities of the two proteins have been compared. Both bind polyuridylic acid and stimulate polypeptide synthesis on this template by S1‐depleted ribosomes. Furthermore, S1 inhibits the translation of the coat protein cistron on MS2 RNA, while it has little effect on the overall translation of T4 mRNA, two characteristic properties of interference factor i.S1 and factor i are thus physically and functionally indistinguishable, which suggests that they have identical polypeptide chains. Implications are discussed in terms of function.

Từ khóa


Tài liệu tham khảo

Revel M., 1973, The Mechanism of Protein Synthesis and Its Regulation, 87

10.1016/S0300-9084(73)80235-4

10.1038/newbio239016a0

Jay G., 1974, Proc. Natl. Acad. Sci. Sci. U.S.A.

10.1111/j.1432-1033.1972.tb02498.x

10.1038/newbio239019a0

10.1101/SQB.1969.034.01.007

Vovnow P., 1971, Biochemistry, 10, 517, 10.1021/bi00779a026

10.1007/BF00333861

10.1007/BF00269653

10.1016/0005-2787(72)90452-2

10.1016/S0021-9258(19)43564-3

10.1016/0014-5793(72)80383-1

10.1111/j.1432-1033.1973.tb02619.x

10.1111/j.1432-1033.1971.tb01504.x

10.1016/0006-291X(73)90175-7

10.1016/0022-2836(67)90286-0

10.1021/bi00835a031

10.1038/195281a0

10.1111/j.1749-6632.1964.tb14213.x

10.1038/227680a0

Avital S., 1973, Anal. Biochem.

Mitchell W. M., 1967, Biochim. Biophys. Acta, 147, 175, 10.1016/0005-2795(67)90101-8

10.1016/0003-9861(68)90569-9

10.1021/bi00835a032

10.1073/pnas.67.4.1909

10.1016/0014-5793(73)80711-2

10.1111/j.1432-1033.1972.tb01660.x

10.1016/S0076-6879(67)11029-X

10.1016/0022-2836(68)90420-8

Kaltschmidt E., 1970, Mol. Gen. Genet., 109, 282

10.1016/0022-2836(71)90456-6

10.1073/pnas.61.2.719

Yaguchi M., 1973, Can. J. Biochem., 57, 1215, 10.1139/o73-160

10.1007/BF00582227

10.1016/S0021-9258(19)43745-9

10.1146/annurev.bi.41.070172.002113

Lee‐Huang S., 1973, Proc. Natl. Acad. Sci. U. S. A., 70, 2874, 10.1073/pnas.70.10.2874

10.1007/BF00569784

10.1016/S0021-9258(18)94333-4

Smolarsky M., 1969, Biochim. Biophys. Acta, 799, 447