Phylogenetic diversity of the <i>dddP</i> gene for dimethylsulfoniopropionate-dependent dimethyl sulfide synthesis in mangrove soils

Canadian Journal of Microbiology - Tập 58 Số 4 - Trang 523-530 - 2012
Mengjun Peng1, Qingyi Xie2, Hu Huo1, Kui Hong2, Jonathan D. Todd3, Andrew Johnston3, Youguo Li1
1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, People’s Republic of China
2Institute of Tropical Bioscience and Biotechnology, Chinese Academy Tropical Agriculture Sciences, Haikou, People’s Republic of China.
3School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK

Tóm tắt

The dddP gene encodes an enzyme that cleaves dimethylsulfoniopropionate (DMSP) into dimethyl sulfide (DMS) plus acrylate and has been identified in various marine bacteria and some fungi. The diversity of dddP genes was investigated by culture-independent PCR-based analysis of metagenomic DNA extracted from 4 mangrove soils in Southern China. A phylogenetic tree of 144 cloned dddP sequences comprised 7 groups, 3 of which also included dddP genes from previously identified Ddd+ (DMSP-dependent DMS production) bacteria. However, most (69%) of the DddP sequences from the mangroves were in 4 other subgroups that did not include sequences from known bacteria, demonstrating a high level of diversity of this gene in these environments. Each clade contained clones from all of the sample sites, suggesting that different dddP types are widespread in mangroves of different geographical locations. Furthermore, it was found the dddP genotype distribution was remarkably influenced by the soil properties pH, available sulfur, salt, and total nitrogen.

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Canadian Journal of Microbiology

Tập 58 Số 4

523-530

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