Photothermally Controlled Gene Delivery by Reduced Graphene Oxide–Polyethylenimine Nanocomposite

Small - Tập 10 Số 1 - Trang 117-126 - 2014
Hyunwoo Kim1,2, Won Jong Kim1,2
1Center for Self-assembly and Complexity, Institute for Basic Science, 70, Yuseong-daero 1689-gil, Yusung-gu, Daejeon, 305-811, Korea
2Department of Chemistry, Pohang University of Science and Technology, San 31 Hyoja-Dong, Pohang 790-784, Korea

Tóm tắt

AbstractExternally stimuli‐triggered spatially and temporally controlled gene delivery can play a pivotal role in achieving targeted gene delivery with maximized therapeutic efficacy. In this study, a photothermally controlled gene delivery carrier is developed by conjugating low molecular‐weight branched polyethylenimine (BPEI) and reduced graphene oxide (rGO) via a hydrophilic polyethylene glycol (PEG) spacer. This PEG–BPEI–rGO nanocomposite forms a stable nano‐sized complex with plasmid DNA (pDNA), as confirmed by physicochemical studies. For the in vitro gene transfection study, PEG–BPEI–rGO shows a higher gene transfection efficiency without observable cytotoxicity compared to unmodified controls in PC‐3 and NIH/3T3 cells. Moreover, the PEG–BPEI–rGO nanocomposite demonstrates an enhanced gene transfection efficiency upon NIR irradiation, which is attributed to accelerated endosomal escape of polyplexes augmented by locally induced heat. The endosomal escaping effect of the nanocomposite is investigated using Bafilomycin A1, a proton sponge effect inhibitor. The developed photothermally controlled gene carrier has the potential for spatial and temporal site‐specific gene delivery.

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Tài liệu tham khảo

10.1126/science.8493530

10.1006/mthe.2000.0044

10.1016/S0169-409X(02)00046-7

10.1021/ar200248u

10.1038/sj.gt.3302024

10.1007/s11095-008-9774-1

10.1016/j.jconrel.2010.08.011

10.2533/chimia.1997.34

10.1038/nrd1775

10.1021/bm801127d

10.1002/mabi.200800248

10.1021/ar200227n

10.1002/anie.200461458

10.1016/j.jconrel.2007.08.025

10.1038/sj.gt.3302323

10.1016/j.biomaterials.2010.01.123

10.1016/j.addr.2003.08.016

10.1016/j.jconrel.2010.06.008

10.1016/j.jconrel.2010.04.012

10.1016/S1011-1344(02)00246-4

10.1038/sj.bjc.6600138

10.1038/86684

10.1002/anie.201000611

10.1039/C1CS15184C

10.1039/b500337g

10.1007/s12274-010-0045-1

10.1021/nn900904h

10.1002/adma.201104864

10.1158/0008-5472.CAN-09-3379

10.1002/smll.201200104

10.1016/j.tibtech.2011.01.008

10.1002/smll.201200264

10.1002/anie.200901479

10.1021/ja803688x

10.1002/smll.200901680

10.1021/bc200397j

10.1002/smll.201100191

10.1021/nl100996u

10.1002/anie.201200474

10.1021/ja906730d

10.1021/cm060258

10.1088/0957-4484/22/5/055705

Moon I. K., 2012, Nat. Commun., 1, 73, 10.1038/ncomms1067

10.1039/c0cc05005a

10.1016/j.biomaterials.2011.11.064

10.1021/ja2010175

10.1039/c2jm31396k

10.1038/ncomms1643

10.1038/nmat2858

10.1038/nnano.2010.90

10.1063/1.2809413

10.1038/nchem.907

10.1016/j.carbon.2011.11.010

10.1021/jp104775z

10.1039/c1sm00011j

10.1021/la0700826

10.1016/j.biomaterials.2010.08.096

10.1021/bc900374d

10.1016/j.biomaterials.2010.12.018

10.1016/S0021-9258(17)32447-X

10.1073/pnas.85.21.7972

10.1016/j.biomaterials.2010.04.048

10.1128/JVI.72.12.9645-9655.1998

10.1016/S0731-7085(02)00571-X

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Small

Tập 10 Số 1

117-126

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