Liên kết phospholipid của kháng thể kháng phospholipid và protein chống đông thai nghén

Springer Science and Business Media LLC - Tập 12 - Trang 27-35 - 1992
Lisa R. Sammaritano1, Azzudin E. Gharavi1, Consolacion Soberano1, Roger A. Levy1, Michael D. Lockshin2
1Division of Rheumatic Diseases, Hospital for Special Surgery—Cornell University Medical Center, New York
2Jational Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda

Tóm tắt

Chúng tôi đã đánh giá sự tương tác của kháng thể kháng phospholipid (aPL) với protein chống đông thai nghén I (PAP I), một protein liên kết phospholipid phụ thuộc vào canxi có thể hoạt động như một chất chống đông tự nhiên. Các thử nghiệm đông máu cho thấy sự kéo dài thời gian đông máu cộng thêm với aPL và PAP I. Các nghiên cứu ELISA và liên kết phospholipid vesicle cho thấy PAP I ức chế sự liên kết của aPL với phospholipid nhưng không ức chế sự liên kết của PAP I với phospholipid bởi aPL. aPL và PAP I tương tác cộng thêm trong hoạt động chống đông trong các hệ thống đông máu in vitro và cạnh tranh để chiếm phospholipid trong hệ thống ELISA. Những dữ liệu này hỗ trợ giả thuyết rằng aPL và PAP I có thể nhận diện các epitopes phospholipid tương tự và rằng sự tương tác in vivo có thể xảy ra.

Từ khóa

#kháng thể kháng phospholipid #protein chống đông thai nghén #phospholipid #hoạt tính chống đông #epitope

Tài liệu tham khảo

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