Phospholipase C5 (NPC5) is involved in galactolipid accumulation during phosphate limitation in leaves of Arabidopsis
Tóm tắt
The replacement of phospholipids by galacto‐ and sulfolipids in plant membranes represents an important adaptive process for growth on phosphate‐limiting soils. Gene expression and lipid analyses revealed that the MYB transcription factor PHR1 that has been previously shown to regulate phosphate responses is not a major factor controlling membrane lipid changes. Candidate genes for phospholipid degradation were selected based on induction of expression during phosphate deprivation. Lipid measurements in the corresponding Arabidopsis mutants revealed that the non‐specific phospholipase C5 (NPC5) is required for normal accumulation of digalactosyldiacylglycerol (DGDG) during phosphate limitation in leaves. The growth and DGDG content of a double mutant
Từ khóa
Tài liệu tham khảo
Dörmann P., 1995, Isolation and characterization of an Arabidopsis mutant deficient in the thylakoid lipid digalactosyl diacylglycerol, Plant Cell, 7, 1801
Sambrook J., 1989, Molecular Cloning: A Laboratory Manual