Phenotypic, genotypic, and symbiotic diversities in strains nodulating clover in different soils in Spain

Canadian Journal of Microbiology - Tập 55 Số 10 - Trang 1207-1216 - 2009
Martha-Helena Ramírez-Bahena1,2, Encarna Velázquez1,2, Félix Fernández-Santos1,2, Álvaro Péix1,2, Eustoquio Martínez‐Molina1,2, Pedro F. Mateos1,2
1Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain
2Instituto de Recursos Naturales y Agrobiología, IRNASA (CSIC), Salamanca, Spain

Tóm tắt

Trifolium species are the most common legumes present in wild Spanish soils; however, there are no studies to date on the diversity of rhizobia nodulating clover in Spain. Twenty strains from different Spanish soils with acidic, neutral, and basic pH were selected to study their genotypic, phenotypic, and symbiotic features. The results showed that the isolates were genotypically diverse, displaying 12 different DNA fingerprint patterns and also 14 different plasmid profiles. Although they have 16S rRNA gene sequences that are nearly identical to that of the type strain of Rhizobium leguminosarum , their recA and atpD gene sequences were phylogenetically divergent from those of R. leguminosarum reference strains, and phenotypic divergence as well as different host ranges were also found. Although most of them nodulated both Trifolium and Phaseolus , only 5 strains were also able to nodulate Pisum . The results of the effectiveness analysis showed a high variability in the symbiotic characteristics of our strains and suggested that Pisum is the more restrictive host of this group. Interestingly, some of the Trifolium isolates showed an ability to promote growth of Pisum in the absence of nodulation.

Từ khóa


Tài liệu tham khảo

10.1016/S0022-2836(05)80360-2

Bergersen F.J., 1961, Aust. J. Biol. Sci., 14, 349, 10.1071/BI9610349

10.1016/j.envpol.2006.10.013

10.1016/S1369-5266(99)80054-5

10.1371/journal.pone.0002567

10.1139/m93-096

10.1111/j.1365-2672.2006.03196.x

10.1016/j.ympev.2006.01.004

Gaunt M.W., 2001, Int. J. Syst. Evol. Microbiol., 51, 2037, 10.1099/00207713-51-6-2037

10.1099/00221287-134-10-2721

10.1111/j.1574-6968.2007.00968.x

10.1007/BF01731581

Kuykendall, L.D. 2005. Family I. Rhizobiaceae Conn 1938, 321AL. In Bergey’s manual of systematic bacteriology. Vol. 2. Part C. Edited by D.J. Brenner, N.R. Krieg, and J.T. Stanley. Springer, New York. pp. 324–361.

10.1007/s00203-007-0209-x

10.1007/s00248-006-9196-8

10.1007/s00284-005-0296-3

10.1016/j.syapm.2005.03.009

10.1016/S0960-8524(02)00055-X

Plazinski J., 1985, Appl. Environ. Microbiol., 49, 1001, 10.1128/AEM.49.4.1001-1003.1985

10.1099/ijs.0.65621-0

10.1099/00221287-88-2-223

10.1007/s11104-006-9062-y

Saitou N., 1987, Mol. Biol. Evol., 4, 406

10.1007/s00203-007-0313-y

10.1139/cjm-47-1-81

Somasegaran, P., and Hoben, H.J. 1994. Handbook for rhizobia. Methods in legume–Rhizobium technology. Springer-Verlag, New York.

10.1093/molbev/msm092

10.1023/A:1026408928279

10.1093/nar/25.24.4876

Thurman N.P., 1985, J. Appl. Bacteriol., 58, 1, 10.1111/j.1365-2672.1985.tb01422.x

10.1007/BF00331660

10.1099/ijs.0.64402-0

Vincent, J.M. 1970. The cultivation, isolation, and maintenance of rhizobia. In A manual for the practical study of the root-nodule bacteria. Edited by J.M. Vincent. Blackwell Scientific Publications, Oxford. pp. 1–13.

Yanni Y., 2001, Aust. J. Plant Physiol., 28, 845

Thông tin
Thông tin xuất bản

Canadian Journal of Microbiology

Tập 55 Số 10

1207-1216

Thông tin tác giả