Phase-contrast microscopy of fresh and cultured pancreatic islet cells of guinea-pigs
Tóm tắt
Isolated cells were prepared from microdissected pancreatic islets of guinea-pigs. Phase-contrast microscopy of the fresh islet cells suspended in a balanced salt solution displayed a number of cellular details, including cytoplasmic secretion granules. There was morphologic evidence of the survival of islet cells in monolayer cultures for up to 3 weeks. A moderate proliferation of cells occurred during the first 2 weeks after explantation. Different types of islet cells could not be distinguished in the phase-contrast microscope.
Tài liệu tham khảo
Bensley, R. R.: Studies on the pancreas of the guinea-pig. Amer. J. Anat. 12, 297–388 (1911).
Drukker, J.: The cultivation of embryonic chick pancreas. Morphological and physiological aspects. Acta morph. neerl.-scand. 5, 316–328 (1964).
Hellerström, C.: A method for the microdissection of intact pancreatic islets of mammals. Acta endocr. (Kbh.) 45, 122–132 (1964).
Hellman, B., and B. Petersson: The activity of the islet B cells, as indicated by the nuclear and nucleolar size in the American obese-hyperglycemic mice. Acta path. microbiol. scand. 50, 291–296 (1960).
Holter, H.: Technique of the Cartesian diver. C. R. Lab. Carlsberg, Sér. Chim., 24, 399–478 (1943).
Jönsson, L. E., J. Pontén, and J. Thorell: Long term production of insulin by adult rat pancreas in vitro. Diabetologia 2, 157–161 (1966).
Keen, H., R. Sells, and R. J. Jarrett: A method for the study of the metabolism of isolated mammalian islets of Langerhans and some preliminary results. Diabetologia 1, 28–32 (1965).
Lacy, P. E., and M. Kostianovsky: Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16, 35–39 (1967).
Lazarus, S. S., and B. Volk: The pancreas in human and experimental diabetes. New York: Grune & Stratton 1962.
Moskalewski, S.: Isolation and culture of the islets of Langerhans of the guinea-pig. Gen. comp. Endocr. 5, 342–353 (1965).
Murell, L. R.: Mammalian pancreatic islet tissue in organ culture. I. Methods of culture and in vitro histogenesis. Exp. Cell. Res. 41, 350–364 (1966).
—, C. R. Morgan, and A. Lazarow: Mammalian pancreatic islet tissue in organ culture. II. Insulin contents of tissues and culture medium. Exp. Cell Res. 41, 365–375 (1966).
O'Leary, J. L.: An experimental study of the islet cells of the pancreas in vivo. Anat. Rec. 45, 27–58 (1930).
Petersson, B.: Isolation and characterization of different types of pancreatic islet cells in guinea-pigs. Acta endocr. (Kbh.) 53, 480–488 (1966a).
—: Cytochemical studies of hydrolytic and oxidative enzymes in sections and isolated cells from the endocrine pancreas of guinea-pigs. Histochemie 7, 116–131 (1966b).
Sandritter, W., K. Federlin, and E. F. Pfeiffer: Quantitative histochemical studies on islet cells. In: S. Brolin, B. Hellman and H. Knutson (eds.), The structure and metabolism of the pancreatic islets, p. 67–74. London: Pergamon Press 1964.
Sandström, B.: A method of cultivating morphologically highly differentiated liver epithelium in vitro. Acta Soc. Med. upsalien 69, 209–214 (1964).
—: Studies on cells from liver tissue cultivated in vitro. Exp. Cell Res. 37, 552–568 (1965).
Vecchio, D., A. Luyckx, G. R. Zahnd, and A. E. Renold: Insulin release induced by glucagon in organ cultures of fetal rat pancreas. Metabolism 15, 577–581 (1966).
Wells, L. J., and E. Borghese: Development of the pancreas of the mouse embryo in vitro: acini and islets. Gen. comp. Endocr. 3, 265–273 (1963).