Periodontal Ligament Cells Under Mechanical Stress Induce Osteoclastogenesis by Receptor Activator of Nuclear Factor κB Ligand Up-Regulation via Prostaglandin E2 Synthesis

Oxford University Press (OUP) - Tập 17 Số 2 - Trang 210-220 - 2002
Hiroyuki Kanzaki1, Mirei Chiba1, Yasutomo Shimizu2, Hideo Mitani1
1Department of Life-Long Oral Health Science, Division of Orthodontics, Graduate School of Dentistry, Tohoku University, Sendai, Japan
2Division of Oral Microbiology and Immunology, Graduate School of Dentistry, Tohoku University, Sendai, Japan

Tóm tắt

Abstract Previously, we discovered that periodontal ligament (PDL) cells not only support osteoclastogenesis through cell-to-cell contact, but also inhibit the formation of tartrate-resistant acid phosphatase-positive (TRAP+) multinucleated cells by a producing soluble factor(s). Furthermore, PDL cells express both receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegerin (OPG) messenger RNA (mRNA). Clinically, “ankylosed teeth,” which lack periodontal ligament, cannot be moved with orthodontic tooth treatment. From this, we hypothesized that PDL cells under mechanical stress should play a pivotal role in osteoclast formation during orthodontic tooth movement. This study examined how mechanical stress affects the osteoclastogenesis-supporting activity of PDL cells. PDL cells were compressed continuously and then cocultured with peripheral blood mononuclear cells (PBMCs) for 4 weeks. PDL cells under mechanical stress up-regulated osteoclastogenesis from PBMCs. Furthermore, the expression of RANKL mRNA and protein in PDL cells increased with compressive force in parallel with the change in the number of osteoclasts. In addition, cyclo-oxygenase 2 (COX-2) mRNA expression was induced by compressive force, and indomethacin inhibited the RANKL up-regulation resulting from compressive force. PDL cells under compressive force exhibited significantly increased prostaglandin E2 (PGE2) production in comparison with control PDL cells. Exogenous PGE2 treatment increased RANKL mRNA expression in PDL cells. Interestingly, OPG expression remained constant throughout compressive force or PGE2 treatment. In conclusion, compressive force up-regulated RANKL expression in PDL cells. Furthermore, RANKL up-regulation in mechanically stressed PDL cells was dependent on PGE2.

Từ khóa


Tài liệu tham khảo

Roberts, 1981, Cellular response to orthodontic force, Dent Clin North Am, 25, 3, 10.1016/S0011-8532(22)02820-8

Mitchell, 1975, Attempted orthodontic movement in the presence of suspected ankylosis, Am J Orthod, 68, 404, 10.1016/0002-9416(75)90181-5

Lekic, 1996, Periodontal ligament cell populations: The central role of fibroblasts in creating a unique tissue, Anat Rec, 245, 327, 10.1002/(SICI)1097-0185(199606)245:2<327::AID-AR15>3.0.CO;2-R

Davidovitch, 1988, Neurotransmitters, cytokines, and the control of alveolar bone remodeling in orthodontics, Dent Clin North Am, 32, 411, 10.1016/S0011-8532(22)00320-2

Udagawa, 1999, Osteoblasts/stromal cells stimulate osteoclast activation through expression of osteoclast differentiation factor/RANKL but not macrophage colony-stimulating factor, Bone, 25, 517, 10.1016/S8756-3282(99)00210-0

Suda, 1992, Modulation of osteoclast differentiation, Endocrine Reviews, 13, 66

Matsuzaki, 1998, Osteoclast differentiation factor (ODF) induces osteoclast-like cell formation in human peripheral blood mononuclear cell cultures, Biochem Biophys Res Commun, 246, 199, 10.1006/bbrc.1998.8586

Jimi, 1996, Osteoclast function is activated by osteoblastic cells through a mechanism involving cell-to-cell contact, Endocrinology, 137, 2187, 10.1210/endo.137.5.8612568

Yasuda, 1999, A novel molecular mechanism modulating osteoclast differentiation and function, Bone, 25, 109, 10.1016/S8756-3282(99)00121-0

Simonet, 1997, Osteoprotegerin: A novel secreted protein involved in the regulation of bone density, Cell, 89, 309, 10.1016/S0092-8674(00)80209-3

Shimizu, 1992, Stimulation by interleukin-1 of interleukin-6 production by human periodontal ligament cells, Arch Oral Biol, 37, 743, 10.1016/0003-9969(92)90081-I

Rasmussen, 2000, Characterization of bone resorbing activity in gingival crevicular fluid from patients with periodontitis, J Clin Periodontol, 27, 41, 10.1034/j.1600-051x.2000.027001041.x

Kong, 1999, Activated T cells regulate bone loss and joint destruction in adjuvant arthritis through osteoprotegerin ligand, Nature, 402, 304, 10.1038/46303

Teng, 2000, Functional human T-cell immunity and osteoprotegerin ligand control alveolar bone destruction in periodontal infection, J Clin Invest, 106, R59, 10.1172/JCI10763

Lowney, 1995, Orthodontic forces increase tumor necrosis factor α in the human gingival sulcus, Am J Orthod, 108, 519, 10.1016/S0889-5406(95)70052-8

Bumann, 1997, Collagen synthesis from human PDL cells following orthodontic tooth movement, Eur J Orthod, 19, 29, 10.1093/ejo/19.1.29

Yamaguchi, 1994, Effect of different magnitudes of tension force on prostaglandin E2 production by human periodontal ligament cells, Arch Oral Biol, 39, 877, 10.1016/0003-9969(94)90019-1

Yamaguchi, 1994, Identification of factors mediating the decrease of alkaline phosphatase activity caused by tension-force in periodontal ligament cells, Gen Pharmacol, 25, 1229, 10.1016/0306-3623(94)90142-2

Shimizu, 1994, Cyclic-tension force stimulates interleukin-1â production by human periodontal ligament cells, J Periodontal Res, 29, 328, 10.1111/j.1600-0765.1994.tb01230.x

Yamaguchi, 1996, Effects of different magnitudes of tension-force on alkaline phosphatase activity in periodontal ligament cells, J Dent Res, 75, 889, 10.1177/00220345960750030501

Spangler, 1996, Cyclooxygenase 1 and 2 in rheumatic disease: Implications for nonsteroidal anti-inflammatory drug therapy, Semin Arthritis Rheum, 26, 435, 10.1016/S0049-0172(96)80024-2

Lowney, 1995, Orthodontic forces increase tumor necrosis factor alpha in the human gingival sulcus, Am J Orthod, 108, 519, 10.1016/S0889-5406(95)70052-8

Shimizu, 1998, Induction of COX-2 expression by mechanical tension force in human periodontal ligament cells, J Periodontol, 69, 670, 10.1902/jop.1998.69.6.670

Kanzaki, 2001, Dual regulation of osteoclast differentiation by periodontal ligament cells through RANKL stimulation and OPG inhibition, J Dent Res, 80, 887, 10.1177/00220345010800030801

Brunette, 1976, Culture and origin of epithelium-like and fibroblast-like cells from porcine periodontal ligament explants and cell suspensions, Arch Oral Biol, 21, 393, 10.1016/0003-9969(76)90001-7

Kanai, 1992, Initial effects of continuously applied compressive stress to human periodontal ligament fibroblasts, J Jpn Orthod Soc, 51, 153

Watanabe, 1998, Effects of conditioned medium of continuously compressed human periodontal ligament fibroblasts on MC3T3-E1 cells, J Jpn Orthod Soc, 57, 173

Nagai, 1999, Reciprocal gene expression of osteoclastogenesis inhibitory factor and osteoclast differentiation factor regulates osteoclast formation, Biochem Biophys Res Commun, 257, 719, 10.1006/bbrc.1999.0524

Helena, 1998, Regulation of osteoprotegerin mRNA levels by prostaglandin E2 in human bone marrow stroma cells, Biochem Biophys Res Commun, 247, 338, 10.1006/bbrc.1998.8783

Charles, 1999, STAT3 activation in stromal/osteoblastic cells is required for induction of the receptor activator of nf-kb ligand and stimulation of osteoclastogenesis by gp130-utilizing cytokines or interleukin-1 but not 1,25-dihydroxyvitamin d3 or parathyroid hormone J, Biol Chem, 274, 19301, 10.1074/jbc.274.27.19301

Takayanagi, 2000, T-cell-mediated regulation of osteoclastogenesis by signaling cross-talk between RANKL and IFN-gamma, Nature, 408, 535, 10.1038/35046102

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Oxford University Press (OUP)

Tập 17 Số 2

210-220

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