Pathogenicity of migratory endoparasitic nematodes on coffee seedlings (Coffea arabica cv. K7) in Australia
Tóm tắt
Plant-parasitic nematodes cause significant losses to coffee globally, but to date, there have been no reports of nematodes causing adverse effects to coffee crops in Australia. Four species of migratory endoparasitic nematodes, including Pratylenchus coffeae, Pratylenchus brachyurus, Pratylenchus zeae, and Radopholus similis, were detected in coffee-growing areas of Australia and their molecular data were also deposited in GenBank under accession numbers MN796428, MN718729, MN831891, and MN796426 respectively. A pathogenicity test of these nematode species was performed on seedlings of Coffea arabica cv. K7 under glasshouse conditions. We found that seedlings inoculated with P. coffeae and R. similis (both isolated from banana roots) and P. brachyurus (isolated from coffee roots), were stunted and their root systems were severely damaged. Tap roots and lateral roots of Coffea arabica cv. K7 were destroyed by P. coffeae, whereas P. brachyurus, and R. similis mainly damaged tap roots. Pratylenchus zeae, originally isolated from Saccharum spp., was not pathogenic to Coffea arabica cv. K7. This is the first report of nematode species capable of damaging coffee in Australia and the results demonstrate that nematodes pose a potential threat to coffee production.
Tài liệu tham khảo
Baines RC, Miyakawa T, Cameron JW, Small RH (1969) Infectivity of two biotypes of the citrus nematode on citrus and on some other hosts. J Nematol 1:150–159
Battistini A, Catalano L, Di Vito M (2001) Response of Prunus rootstocks to root-knot (Meloidogyne spp.) and root-lesion (Pratylenchus vulnus) nematodes. In: The Fifth International Peach Symposium, pp 663–668
Bell CA, Atkinson HJ, Andrade AC, Nguyen HX, Swibawa IG, Urwin PE (2018) A high-throughput molecular pipeline reveals the diversity in prevalence and abundance of Pratylenchus and Meloidogyne species in coffee plantations. Phytopathology 108:641–650
Berry SD, Fargette M, Spaull VW, Morand S, Cadet P (2008) Detection and quantification of root-knot nematode (Meloidogyne javanica), lesion nematode (Pratylenchus zeae) and dagger nematode (Xiphinema elongatum) parasites of sugarcane using real-time PCR. Mol Cell Probes 22:168–176
Byrd DW, Kirkpatrick T, Barker KR (1983) An improved technique for clearing and staining plant tissues for detection of nematodes. J Nematol 15:142
Coolen WA, D’Herde CJ (1972) A method for the quantitative extraction of nematodes from plant tissue. State Agriculture Research Centre, Belgium
Fallas G, Sarah J-L, Fargette M (1995) Reproductive fitness and pathogenicity of eight Radopholus similis isolates on banana plants (Musa AAA v. Poyo). Nematropica 25:135–141
Inomoto M, Oliveira C, Mazzafera P, Goncalves W (1998) Effects of Pratylenchus brachyurus and P. coffeae on seedlings of Coffea arabica. J Nematol 30:362–367
Inomoto MM, Kubo RK, Silva RA, Oliveira CMG, Tomazini MDT, Mazzafera PM (2007) Pathogenicity of two Pratylenchus coffeae populations from Brazil on coffee plants. J Nematol 9:853–858. https://doi.org/10.1163/156854107782331144
Inomoto MM, Oliveira CMG (2008) Coffee-Associated Pratylenchus spp.: Ecology and Interactions with Plants. In: Souza RM (ed) Plant-parasitic nematodes of coffee. Springer, pp 51–64
Kubo RK, Oliveira CMG, Antedomenico SR, Monteiro AR, Ferraz CBLC, Inomoto MM (2004) Ocorrência de nematóides do gênero Pratylenchus em cafezais do estado de São Paulo. Nematologia Brasileira 28:159–165
Kumar A, Samuel S (1990) Nematodes attacking coffee and their management-a new review. Journal of Coffee Research 20:1–27
Loof PAA (1991) The Family Pratylenchidae Thorne, 1949. In: Nickle RW (ed) Manual of Agricultural Nematology. Marcel Dekker, New York USA, pp 363–421
Machado ACZ, Ferraz LCCB, Oliveira CMG (2007) Development of a species-specific reverse primer for the molecular diagnostic of Pratylenchus brachyurus. Nematropica 37:249–257
McLeod R, Reay F, Smyth J (1994) Plant nematodes of Australia listed by plant and by genus. NSW Agriculture
Mizukubo T (1995) Evidence for Pratylenchus coffeae races in differential reproduction on fifteen cultivars (Nematoda: Pratylenchidae). Nematological Research (Japanese Journal of Nematology) 25:85–93
Moens T, Araya M, Swennen R, De Waele D, Sandoval J (2003) Effect of growing medium, inoculum density, exposure time and pot volume: Factors affecting the resistance screening for Radopholus similis in banana (Musa spp.). Nematropica 33:9–26
Moody E, Lownsbery B, Ahmed J (1973) Culture of the root-lesion nematode Pratylenchus vulnus on carrot disks. J Nematol 5:225–256
Oliveira CMG, Inomoto MM, Vieira AMC, Monteiro AR (1999) Efeito de densidades populacionais de Pratylenchus brachyurus no crescimento de plântulas de Coffeae arabica cv. Mundo Novo e C. canephora cv. Apoatã. Nematropica 29
Pinochet J (1987) Management of plant parasitic nematodes in Central America: the Panama experience. In: Veech JA, Dickson DW (eds) Vistas on Nematology. Society of Nematology, Hyattsville, Maryland, pp 105–113
Rodriguez M, Sánchez L, Rodriguez M (2000) Plant parasitic nematodes associated with coffee crop (Coffea arabica) in Cajálbana. Cuba Revista de Protección Vegetal 15:38–42
Schenck S, Schmitt DP (1992) Survey of nematodes on coffee in Hawaii. J Nematol 24:771–775
Seinhorst JW (1950) De betekenis van de toestand van de grond voor het optreden van aantasting door het stengelaaltje (Ditylenchus dipsaci (Kühn) Filipjev). Tijdschr PlZiekt 56:289–348
Stanton J, McNicol C, Steele V (1998) Non-manual lysis of second-stage Meloidogyne juveniles for identification of pure and mixed samples based on the polymerase chain reaction. Australas Plant Path 27:112–115
Tagliavia M, Nicosia A, Gianguzza F (2009) Complete decontamination and regeneration of DNA purification silica columns. Anal Biochem 385:182–183
Trinh P, Karssen G, Subbotin S, Waeyenberge L, Nguyen C, Moens M (2004) Radopholus arabocoffeae sp. n. (Nematoda: Pratylenchidae), a nematode pathogenic to Coffea arabica in Vietnam, and additional data on R. duriophilus. Nematology 6:681–693
Trinh PQ, Wesemael WM, Nguyen ST, Nguyen CN, Moens M (2011) Pathogenicity and reproductive fitness of Pratylenchus coffeae and Radopholus arabocoffeae on Arabica coffee seedlings (Coffea arabica cv. Catimor) in Vietnam. Eur J Plant Pathol 130:45–57
Villain L, Lima Salgado SM, Trinh PQ (2018) Nematode Parasites of Coffee and Cocoa. In: Sikora RA, Coyne D, Hallmann J, Timper P (eds) Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, 3rd edn. CABI, Boston, MA, pp 536–583
Whitehead AG, Hemming JR (1965) A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Ann Appl Biol 55:25–38
Wiryadiputra S, Tran KL (2008) Indonesia and Vietnam. In: Souza MR (ed) Plant-parasitic nematodes of coffee. Springer, pp 277–292
Zimmermann A (1898) De nematoden der koffiewortels. Batavia (Jakarta, Indonesia) and S' Gravenhage, La Hague, Pays Bas, G. Kolf & Co., coll. Mededelingen Uit’s Lands Plantentuin 27:67