Passive Immunization with Antibodies against Three Distinct Epitopes onPlasmodium yoeliiMerozoite Surface Protein 1 Suppresses Parasitemia

Infection and Immunity - Tập 66 Số 8 - Trang 3925-3930 - 1998
Lilian Spencer1, Solabomi A. Ogun1, Suzanne L. Fleck1, Irene T. Ling1, Terry J. Scott-Finnigan1, Michael J. Blackman1, Anthony A. Holder1
1Division of Parasitology, National Institute for Medical Research, London NW7 1AA, United Kingdom

Tóm tắt

ABSTRACTWe have produced monoclonal antibodies againstPlasmodium yoeliimerozoite surface protein 1 (MSP-1) and have assessed their ability to suppress blood stage parasitemia by passive immunization. Six immunoglobulin G antibodies were characterized in detail: three (B6, D3, and F5) were effective in suppressing a lethal blood stage challenge infection, two (B10 and G3) were partially effective, and one (B4) was ineffective. MSP-1 is the precursor to a complex of polypeptides on the merozoite surface; all of the antibodies bound to this precursor and to an ∼42-kDa fragment (MSP-142) that is derived from the C terminus of MSP-1. MSP-142is further cleaved to an N-terminal ∼33-kDa polypeptide (MSP-133) and a C-terminal ∼19-kDa polypeptide (MSP-119) comprised of two epidermal growth factor (EGF)-like modules. D3 reacted with MSP-142but not with either of the constituents MSP-133and MSP-119, B4 recognized an epitope within the N terminus of MSP-133, and B6, B10, F5, and G3 bound to MSP-119. B10 and G3 bound to epitopes that required both C-terminal EGF-like modules for their formation, whereas B6 and F5 bound to epitopes in the first EGF-like module. These results indicate that at least three distinct epitopes onP. yoeliiMSP-1 are recognized by antibodies that suppress parasitemia in vivo.

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Tài liệu tham khảo

Akerstrom B. Brodin T. Reis K. Bjorck L. Protein G: a powerful tool for binding and detection of monoclonal and polyclonal antibodies.J. Immunol.135198525892592

10.1084/jem.172.1.379

10.1016/0166-6851(91)90127-R

10.1084/jem.180.1.389

10.1006/expr.1996.0069

Burns J. M. Majarian W. R. Young J. F. Daly T. M. Long C. A. A protective monoclonal antibody recognizes an epitope in the carboxyl-terminal cysteine-rich domain in the precursor of the major merozoite surface antigen of the rodent malarial parasite, Plasmodium yoelii.J. Immunol.143198926702676

10.1006/expr.1996.0007

10.1128/iai.64.1.253-261.1996

10.1128/iai.61.6.2462-2467.1993

Daly T. M. Long C. A. Humoral response to a carboxyl-terminal region of the merozoite surface protein-1 plays a predominant role in controlling blood-stage infection in rodent malaria.J. Immunol.1551995236243

10.1128/iai.64.7.2602-2608.1996

10.1016/0166-6851(84)90071-9

10.1128/iai.64.9.3532-3536.1996

10.1038/284366a0

Freeman R. R. Holder A. A. Characteristics of the protective response of BALB/c mice immunized with a purified Plasmodium yoelii schizont antigen.Clin. Exp. Immunol.541983609616

10.1084/jem.186.10.1689

Hirunpetcharat C. Tian J.-H. Kaslow D. C. van Rooijen N. Kumar S. Berzofsky J. A. Miller L. H. Good M. F. Complete protective immunity induced in mice by immunization with the 19-kilodalton carboxyl-terminal fragment of the merozoite surface protein-1 (MSP119) of Plasmodium yoelii expressed in Sacchromyces cerevisiae.J. Immunol.159199734003411

10.1038/294361a0

10.1084/jem.156.5.1528

10.1017/S0031182000054482

10.1007/BF03401557

10.1016/0166-6851(89)90175-8

10.1111/j.1365-3024.1994.tb00324.x

10.1111/j.1365-3024.1995.tb00910.x

10.1016/S0264-410X(97)00076-5

Majarian W. R. Daly T. M. Weidanz W. P. Long C. A. Passive immunization against murine malaria with IgG3 monoclonal antibody.J. Immunol.132198431313137

10.1016/0166-6851(87)90189-7

10.1016/0166-6851(95)00090-N

10.1128/iai.65.11.4419-4423.1997

10.1016/0378-1119(88)90005-4

Tian J.-H. Miller L. H. Kaslow D. C. Ahlers J. Good M. F. Alling D. W. Berzofsky J. A. Kumar S. Genetic regulation of protective immune response in congenic strains of mice vaccinated with a subunit malaria vaccine.J. Immunol.157199611761183

10.1128/iai.65.8.3032-3036.1997

10.1128/iai.59.10.3547-3554.1991

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Infection and Immunity

Tập 66 Số 8

3925-3930

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