Các gen ortholog tiptop/teashirt và spalt ở động vật chân khớp và vai trò tiềm năng của chúng như các gen lựa chọn "thân"

EvoDevo - Tập 12 Số 1 - 2021
Brenda Irene Medina Jiménez1, Graham E. Budd1, Ralf Janßen1
1Department of Earth Sciences, Palaeobiology, Uppsala University, Villavägen 16, Uppsala, Sweden

Tóm tắt

Tóm tắt Đặt bối cảnh Trong ruồi giấm Drosophila melanogaster, yếu tố phiên mã chứa miền homeobox Teashirt (Tsh) có vẻ xác định danh tính thân thể song song với chức năng của các gen Hox. Trong khi ở Drosophila có một gen thứ hai liên quan chặt chẽ với tsh, gọi là tiptop (tio), thì ở các loài động vật chân khớp khác chỉ tồn tại một bản sao duy nhất (được gọi là tio/tsh). Sự biểu hiện của tshtio/tsh tương ứng, thật bất ngờ, lại rất giống nhau giữa các loài động vật chân khớp, gợi ý rằng chức năng của nó như một gen lựa chọn thân có thể được bảo tồn. Nghiên cứu khác, chẳng hạn như trên bọ Tribolium castaneum, đặt câu hỏi về việc bảo tồn chức năng Tsh giữa các loài côn trùng. Yếu tố phiên mã chứa ngón kẽm Spalt (Sal) liên quan đến sự điều chỉnh Drosophila tsh, nhưng tương tác điều chỉnh này dường như cũng không được bảo tồn trong Tribolium. Tuy nhiên, liệu chức năng và tương tác của tshsal như những tác nhân xác định thân có thể được bảo tồn hay không vẫn chưa rõ ràng vì thiếu các nghiên cứu so sánh về sự biểu hiện của sal (trừ Tribolium), và dữ liệu chức năng (nếu có) chỉ bị giới hạn trong Lớp Insecta. Kết quả Tại đây, chúng tôi cung cấp thêm dữ liệu về sự biểu hiện tsh ở động vật chân khớp, cho thấy dữ liệu đầu tiên về sự biểu hiện tio/tsh ở động vật onychophoran, và cung cấp một nghiên cứu tổng thể về các mẫu biểu hiện sal ở động vật chân khớp và một loài onychophoran. Kết luận Dữ liệu của chúng tôi ủng hộ ý tưởng rằng các gen tio/tsh tham gia vào sự phát triển của các đoạn "thân" bằng cách điều chỉnh sự phát triển chi. Dữ liệu của chúng tôi cho thấy thêm rằng chức năng của Sal thực sự khó có khả năng được bảo tồn trong sự phát triển của thân so với đầu như ở Drosophila, nhưng sự biểu hiện sớm của sal thì hoàn toàn phù hợp với một chức năng homeotic, ít nhất là trong nhóm Arthropoda.

Từ khóa


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