Paenibacillus residui sp. nov., isolated from urban waste compost

International Journal of Systematic and Evolutionary Microbiology - Tập 60 Số 10 - Trang 2415-2419 - 2010
Ivone Vaz‐Moreira1,2, Vânia Figueira1, Ana R. Lopes2, Rüdiger Pukall3, Cathrin Spröer3, Peter Schümann3, Olga C. Nunes2, Célia M. Manaia1
1Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4200-072 Porto, Portugal
2LEPAE, Departamento de Engenharia Química, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal
3DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, D-38124 Braunschweig, Germany

Tóm tắt

Two bacterial strains, MC-246T and MC-247, were isolated from municipal urban waste compost and characterized by a polyphasic approach. Both isolates were Gram-stain-variable, endospore-forming rods that were catalase-, oxidase- and β-galactosidase-positive, and able to grow at 25–50 °C and pH 7.0–9.0, with optimum growth at 37 °C and pH 7. The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0; the major respiratory quinone was menaquinone MK-7; the cell wall peptidoglycan was of type A1γ; and the DNA G+C content was 49 mol%. These characteristics, as well as data from 16S RNA gene sequence analysis, showed that these strains were affiliated with the genus Paenibacillus; the type strains of Paenibacillus ginsengarvi and Paenibacillus hodogayensis were among their closest neighbours (<94.2 % sequence similarity). Nevertheless, the hypothesis that strains MC-246T and MC-247 could represent a novel species was supported by the low 16S rRNA gene sequence similarity values shared with other members of the genus Paenibacillus and by the observation of distinct biochemical and physiological traits. Strains MC-246T and MC-247 shared 99.6 % 16S rRNA gene sequence similarity and showed almost identical MALDI-TOF mass spectra, but could be distinguished at the phenotypic and genotypic level. However, DNA–DNA hybridization between strains MC-246T and MC-247 resulted in values above 70 % indicating that both organisms represent a single species, for which the name Paenibacillus residui sp. nov. is proposed; the type strain is MC-246T (=DSM 22072T =CCUG 57263T).

Từ khóa


Tài liệu tham khảo

Ash, 1993, Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus, Antonie van Leeuwenhoek, 64, 253, 10.1007/BF00873085

Bruce, 1996, Automated system rapidly identifies and characterizes microorganisms in food, Food Technol, 50, 77

Cashion, 1977, A rapid method for the base ratio determination of bacterial DNA, Anal Biochem, 81, 461, 10.1016/0003-2697(77)90720-5

De Ley, 1970, The quantitative measurement of DNA hybridization from renaturation rates, Eur J Biochem, 12, 133, 10.1111/j.1432-1033.1970.tb00830.x

Euzéby, 1997, List of bacterial names with standing in nomenclature: a folder available on the Internet, Int J Syst Bacteriol, 47, 590, 10.1099/00207713-47-2-590

Fehr, 2007, Confirming decentralised composting as a definite option in urban waste management, Int J Environ Technol Manag, 7, 274, 10.1504/IJETM.2007.015145

Ferreira da Silva, 2006, Antibiotic resistance of enterococci and related bacteria in an urban wastewater treatment plant, FEMS Microbiol Ecol, 55, 322, 10.1111/j.1574-6941.2005.00032.x

Ferreira da Silva, 2007, Antimicrobial resistance patterns in Enterobacteriaceae isolated from an urban wastewater treatment plant, FEMS Microbiol Ecol, 60, 166, 10.1111/j.1574-6941.2006.00268.x

Huß, 1983, Studies on the spectrophotometric determination of DNA hybridization from renaturation rates, Syst Appl Microbiol, 4, 184, 10.1016/S0723-2020(83)80048-4

Jukes, 1969, Evolution of protein molecules, In Mammalian Protein Metabolism, vol, 21, 10.1016/B978-1-4832-3211-9.50009-7

Kämpfer, 1996, Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa, Can J Microbiol, 42, 989, 10.1139/m96-128

Mesbah, 1989, Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography, Int J Syst Bacteriol, 39, 159, 10.1099/00207713-39-2-159

Murray, 1994, Determinative and cytological light microscopy, In Methods for General and Molecular Bacteriology, 21

Rhuland, 1955, The behavior of the isomers of α , ϵ-diaminopimelic acid on paper chromatograms, J Am Chem Soc, 77, 4844, 10.1021/ja01623a047

Schleifer, 1972, Peptidoglycan types of bacterial cell walls and their taxonomic implications, Bacteriol Rev, 36, 407, 10.1128/MMBR.36.4.407-477.1972

Schleifer, 1985, Chemical composition and structure of murein, In Chemical Methods in Bacterial Systematics, 201

Shida, 1997, Transfer of Bacillus alginolyticus , Bacillus chondroitinus , Bacillus curdlanolyticus , Bacillus glucanolyticus , Bacillus kobensis , and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus, Int J Syst Bacteriol, 47, 289, 10.1099/00207713-47-2-289

Smibert, 1994, Phenotypic characterization. In Methods for General and Molecular Bacteriology, 607

Takeda, 2002, Paenibacillus koleovorans sp. nov., able to grow on the sheath of Sphaerotilus natans, Int J Syst Evol Microbiol, 52, 1597

Tiago, 2004, Metabolic and genetic diversity of mesophilic and thermophilic bacteria isolated from composted municipal sludge on poly-ϵ-caprolactones, Curr Microbiol, 49, 407, 10.1007/s00284-004-4353-0

Tindall, 1989, Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum, FEMS Microbiol Lett, 60, 251, 10.1111/j.1574-6968.1989.tb03481.x

Tóth, 2008, Wohlfahrtiimonas chitiniclastica gen. nov., sp. nov., a new gammaproteobacterium isolated from Wohlfahrtia magnifica (Diptera: Sarcophagidae), Int J Syst Evol Microbiol, 58, 976, 10.1099/ijs.0.65324-0

Vaz-Moreira, 2007

Vaz-Moreira, 2008, Diversity of bacterial isolates from commercial and homemade composts, Microb Ecol, 55, 714, 10.1007/s00248-007-9314-2

Wayne, 1987, International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics, Int J Syst Bacteriol, 37, 463, 10.1099/00207713-37-4-463

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Thông tin xuất bản

International Journal of Systematic and Evolutionary Microbiology

Tập 60 Số 10

2415-2419

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