PIGMENT SIGNATURES AND PHYLOGENETIC RELATIONSHIPS OF THE PAVLOVOPHYCEAE (HAPTOPHYTA)1

Journal of Phycology - Tập 39 Số 2 - Trang 379-389 - 2003
K. van Lenning1, Mikel Latasa1, Marta Estrada1, Alberto G. Sáez2, Linda Medlin2, Ian Probert3, Benoı̂t Véron3, J. R. Young4
1Institut de Ciències del Mar, Consejo Superior de Investigaciones Científicas, Passeig Marítim 37‐49, 08003 Barcelona, Spain
2Alfred Wegener Institute für Polar und Meeresforschung, Department of Biological Oceanography, Am. Handelshafen 12, D‐27570 Bremerhaven, Germany
3Université de Caen Basse Normandie, Laboratoire de Biologie et Biotechnologies Marines, Esplanade de la Paix, 14032 Caen Cedex, France
4The Natural History Museum, Department of Palaeontology, Cromwell Rd, London SW7 5BD, UK

Tóm tắt

Variations in the HPLC‐derived pigment composition of cultured Pavlovophyceae (Cavalier‐Smith) Green et Medlin were compared with phylogenetic relationships inferred from 18S rDNA sequencing, morphological characteristics, and current taxonomy. The four genera described for this haptophyte class (DiacronemaPrauser emend. Green et Hibberd,ExanthemachrysisLepailleur,PavlovaButcher, andRebeccaGreen) were represented by nine different species (one of which with data from GeneBank only). Chlorophyllsa,c1,c2and MgDVP (Mg‐[3,8‐divinyl]‐phytoporphyrin‐132‐methylcarboxylate) and the carotenoids fucoxanthin, diadinoxanthin, diatoxanthin, and β,β‐carotene were detected in all cultures. Species only differed in the content of an unknown (diadinoxanthin‐like) xanthophyll and two polar chlcforms, identified as a monovinyl (chlc1‐like) and a divinyl (chlc2‐like) compound. This is the first observation of the monovinyl form in haptophytes. Based on distribution of these two chlcforms, species were separated into Pavlovophyceae pigment types A, B, and C. These pigment types crossed taxonomic boundaries at the generic level but were in complete accordance with species groupings based on molecular phylogenetic relationships and certain ultrastructural characteristics (position and nature of pyrenoid, stigma, and flagella). These results suggest that characterization of the pigment signature of unidentified culture strains of Pavlovophyceae can be used to predict their phylogenetic affinities and vice versa. Additional studies have been initiated to evaluate this possibility for the haptophyte class Prymnesiophyceae.

Từ khóa


Tài liệu tham khảo

10.1016/S0031-9422(00)88964-5

Cavalier‐Smith T., 1993, Kingdom Protozoa and its 18 phyla., Microbiol. Rev, 57, 953, 10.1128/mr.57.4.953-994.1993

Cavalier‐Smith T., 1994, The Haptophyte Algae, 413, 10.1093/oso/9780198577720.003.0022

Cosson J., 1987, Croissance des sporophytes issus d'hybridations interspécifiques et intergénériques chez les Laminaires., Crypto. Algol, 8, 61

10.3354/meps076185

10.2216/i0031-8884-39-1-19.1

10.1039/a910345g

Egeland E. S., 1999, Search for other carotenoids with 3‐hydroxy‐4‐keto‐5,6‐epoxy end group‐carotenoids of an unidentified microalga, 39

Falkowski P. G., 1997, Aquatic Photosynthesis, 1

10.1104/pp.91.2.727

10.2307/2412923

10.1039/c39890001827

10.1046/j.1529-8817.2001.037001121.x

Garrido J. L., 1997, Análisis de pigmentos de microalgas marinas mediante cromatografía líquida, 256

10.1046/j.1529-8817.2000.99135.x

10.1002/jhrc.1240160405

10.1046/j.1529-8817.1998.340070.x

10.1111/j.0022-3646.1995.00761.x

Gayral P., 1979, Exanthemachrysis gayraliae Lepailleur (Prymnesiophyceae, Pavlovales). Ultrastructure et discussion taxonomique., Protistologica, 15, 271

10.1080/00071618000650171

10.1017/S0025315400026175

10.1139/b88-208

Green J. C., 1994, The Haptophyte Algae, 47, 10.1093/oso/9780198577720.003.0003

Hori T., 1994, The Haptophyte Algae, 91, 10.1093/oso/9780198577720.003.0005

10.1093/bioinformatics/17.8.754

10.1016/0304-4165(69)90308-0

10.1016/0304-4165(72)90238-3

Jeffrey S. W., 1997, Phytoplankton Pigments in Oceanography—Guidelines to Modern Methods, 566

10.1099/00221287-36-2-277

Jeffrey S. W., 1997, Phytoplankton Pigments in Oceanography—Guidelines to Modern Methods, 37

10.1016/0005-2728(87)90188-5

Jeffrey S. W., 1994, The Haptophyte Algae, 111, 10.1093/oso/9780198577720.003.0006

10.1017/S0025315400035736

Kalina T., 1975, Taxonomie und Ultrastrukture der Art Corcontochrysis noctivaga (Haptophyceae)., Preslia, 47, 1

10.1007/BF01731581

10.1007/BF02491072

Lockhart P. J., 1994, Recovering evolutionary trees under a more realistic model of sequence evolution., Mol. Biol. Evol, 11, 605

10.3354/meps144265

Maddison W. P., 1992, MacClade: Analysis of Phylogeny and Character Evolution. Version 3

10.1016/0378-1119(88)90066-2

10.1007/978-3-7091-6542-3_11

10.1111/j.0022-3646.1989.00761.x

10.1016/0305-1978(74)90016-7

Porra R. J., 1997, Phytoplankton Pigments in Oceanography—Guidelines to Modern Methods, 85

Posada D., 1998, Modeltest: testing the model of DNA substitution., Bioinformatics, 14, 917, 10.1093/bioinformatics/14.9.817

Rodríguez F., 2002, Aplicación del análisis de pigmentos por cromatografía líquida de alta eficacia (HPLC) al estudio de la compocición y distribución del fitoplancton marino, 264

10.1016/S0022-5193(05)80104-3

10.1046/j.1529-8817.2000.99158.x

Swofford D. L., 2000, PAUP*

10.1029/96PA03934

10.1093/oxfordjournals.molbev.a026350

10.1093/nar/25.24.4876

Van Der Veer J., 1977, Pavlova ennorea sp. nov. a haptophyceaen alga with a dominant palmelloid phase from England., Acta Bot. Neerl, 26, 159, 10.1111/j.1438-8677.1977.tb01108.x

10.1080/09670269600651401

10.1111/j.0022-3646.1997.01016.x

10.3354/meps219085

Zapata M., 1998, Harmful Algae, 304

10.1007/BF02279480

Zapata M. Rodríguez F. & Fraga S. 2000a. Pigment patterns of toxic and non‐toxicPseudo‐Nitzschiaspecies (Bacillariophyceae). Harmful Algal Blooms Poster abstract Ninth Conference Tasmania Australia p. 251.

10.3354/meps195029

Thông tin
Thông tin xuất bản

Journal of Phycology

Tập 39 Số 2

379-389

Thông tin tác giả