PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) regulates auxin biosynthesis at high temperature

Proceedings of the National Academy of Sciences of the United States of America - Tập 108 Số 50 - Trang 20231-20235 - 2011
Keara A. Franklin1, Sang Ho Lee2, Dhaval Patel1, S. Vinod Kumar3, Angela Spartz2, Chen Gu4, Songqing Ye5, Peng Yu2, Gordon Breen1, Jerry D. Cohen5,6, Philip A. Wigge3, William M. Gray2,6
1School of Biological Sciences, University of Bristol, Bristol Bs8 1Ug, United Kingdom
2Department of Plant Biology, University of Minnesota, St. Paul, MN 55108;
3Department of Cell and Developmental Biology, John Innes Centre, Norwich, NR4 7UH, United Kingdom
4Department of Biology, Macalester College, St. Paul, MN 55105;
5Department of Horticultural Science, University of Minnesota, St. Paul, MN 55108; and
6Microbial and Plant Genomics Institute, University of Minnesota, St. Paul, MN 55108

Tóm tắt

At high ambient temperature, plants display dramatic stem elongation in an adaptive response to heat. This response is mediated by elevated levels of the phytohormone auxin and requires auxin biosynthesis, signaling, and transport pathways. The mechanisms by which higher temperature results in greater auxin accumulation are unknown, however. A basic helix-loop-helix transcription factor, PHYTOCHROME-INTERACTING FACTOR 4 (PIF4), is also required for hypocotyl elongation in response to high temperature. PIF4 also acts redundantly with its homolog, PIF5, to regulate diurnal growth rhythms and elongation responses to the threat of vegetative shade. PIF4 activity is reportedly limited in part by binding to both the basic helix-loop-helix protein LONG HYPOCOTYL IN FAR RED 1 and the DELLA family of growth-repressing proteins. Despite the importance of PIF4 in integrating multiple environmental signals, the mechanisms by which PIF4 controls growth are unknown. Here we demonstrate that PIF4 regulates levels of auxin and the expression of key auxin biosynthesis genes at high temperature. We also identify a family of SMALL AUXIN UP RNA ( SAU R ) genes that are expressed at high temperature in a PIF4 -dependent manner and promote elongation growth. Taken together, our results demonstrate direct molecular links among PIF4, auxin, and elongation growth at high temperature.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 108 Số 50

20231-20235

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