PHOTOADAPTATION AND THE “PACKAGE” EFFECT IN <i>DUNALIELLA TERTIOLECTA</i> (CHLOROPHYCEAE)<sup>1</sup>

Journal of Phycology - Tập 25 Số 1 - Trang 70-78 - 1989
T. Berner1, Zvy Dubinsky1, Kevin Wyman2, Paul G. Falkowski2
1Department of Life Sciences, Bar Ilan University, Ramat Can, 52000 Israel
2Oceanographic Sciences Division, Brookhaven National Laboratory, Upton, New York 1973

Tóm tắt

ABSTRACTIn the marine unicellular chlorophyte, Dunaliella tertiolecta Butcher, the spectrally averaged m vivo absorption cross section, normalized to chlorophyll a (so‐called a* values), vary two‐fold in response to changes in growth irradiance. We used a kinetic approach to examine the specific factors which account for these changes in optical properties as cells photoadapt. Using Triton X‐100 to solubilize membranes, we were able to differentiate between “package” effects and pigmentation effects. Our analyses suggest that 43–49% of the variability in a* is due to changes in pigmentation, whereas 51–57% is due to the “package” effect. Further analyses revealed that changes in cell sue did not significantly affect packaging, while thylakoid stacking and the transparency of thylakoid membranes were important factors. Our results suggest that thylakoid membrane protein/lipid ratios change during photoadaptation, and these changes influence the effective rate of light harvesting per unit chlorophyll a.

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Thông tin xuất bản

Journal of Phycology

Tập 25 Số 1

70-78

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