PEGylation of a glycosaminoglycan-binding, dominant-negative CXCL8 mutant retains bioactivity in vitro and in vivo

Adage, 2015, PA401, a novel CXCL8-based biologic therapeutic with increased glycosaminoglycan binding, reduces bronchoalveolar lavage neutrophils and systemic inflammatory markers in a murine model of LPS-induced lung inflammation, Cytokine, 10.1016/j.cyto.2015.08.006 Rollins, 1997, Chemokines, Blood, 90, 909, 10.1182/blood.V90.3.909 Frederick, 2001, Chemokines in cancer, Expert Rev. Mol. Med., 3, 1, 10.1017/S1462399401003301 Baggiolini, 2001, Chemokines in pathology and medicine, J. Intern. Med., 250, 91, 10.1046/j.1365-2796.2001.00867.x Broxmeyer, 1999, 263 Proudfoot, 2006, The biological relevance of chemokine-proteoglycan interactions, Biochem. Soc. Trans., 34, 422, 10.1042/BST0340422 Rek, 2009, Therapeutically targeting protein–glycan interactions, Br. J. Pharmacol., 157, 686, 10.1111/j.1476-5381.2009.00226.x Gandhi, 2008, The structure of glycosaminoglycans and their interactions with proteins, Chem. Biol. Drug Des., 72, 455, 10.1111/j.1747-0285.2008.00741.x Raman, 2010, Chemical tumor biology of heparan sulfate proteoglycans, Curr. Chem. Biol., 4, 20 Gesslbauer, 2007, Proteoglycanomics: tools to unravel the biological function of glycosaminoglycans, Proteomics, 7, 2870, 10.1002/pmic.200700176 Rudd, 2010, The conformation and structure of GAGs: recent progress and perspectives, Curr. Opin. Struct. Biol., 20, 567, 10.1016/j.sbi.2010.08.004 Proudfoot, 2010, Anti-chemokine small molecule drugs: a promising future?, Expert Opin. Invest. Drugs, 19, 345, 10.1517/13543780903535867 Esko, 2001, Molecular diversity of heparan sulfate, J. Clin. Investig., 108, 169, 10.1172/JCI200113530 Nourshargh, 2010, Breaching multiple barriers: leukocyte motility through venular walls and the interstitium, Nat. Rev. Mol. Cell Biol., 11, 366, 10.1038/nrm2889 Lau, 2004, Identification of the Glycosaminoglycan Binding Site of the CC Chemokine, MCP-1 implications for structure and function in vivo, J. Biol. Chem., 279, 22294, 10.1074/jbc.M311224200 Falsone, 2013, Designing CXCL8-based decoy proteins with strong anti-inflammatory activity in vivo, Biosci. Rep., 33, 743, 10.1042/BSR20130069 Gerlza, 2014, A combinatorial approach to biophysically characterise chemokine-glycan binding affinities for drug development, Molecules, 19, 10618, 10.3390/molecules190710618 Gschwandtner, 2017, Glycosaminoglycans are important mediators of neutrophilic inflammation in vivo, Cytokine, 91, 65, 10.1016/j.cyto.2016.12.008 Krieger, 2014, YASARA View - molecular graphics for all devices - from smartphones to workstations, Bioinformatics, 30, 2981, 10.1093/bioinformatics/btu426