1Biochemie der Pflanzen, Ruhr-Universität Bochum, Postfach 102148, D-4630 Bochum 1, Bundesrepublik Deutschland
Tóm tắt
Abstract PAPS-reductase from Escherichia coli was em ployed to detect thioredoxins from pro- and eukaryotic organisms. A simple method for the isolation of this enzyme and properties of the enzymatic assay were described. A comparison betw een thioredoxins detected by the PAPS-reductase and the Fructose-bisphosphatase or NADP malate dehydrogenase was used to assess the validity of the test. The high cross-reactivity of the bacterial enzyme was useful in the purification of heterologous thioredoxins from spinach, Synechococcus, and Saccharomyces cerevisiae.
