Overriding the co‐limiting import of carbon and energy into tuber amyloplasts increases the starch content and yield of transgenic potato plants

Wiley - Tập 6 Số 5 - Trang 453-464 - 2008
Lizhi Zhang1, Rainer E. Häusler1, Christian Greiten1, Mohammad‐Reza Hajirezaei2, Ilka Haferkamp3, H. Ekkehard Neuhaus3, Ulf‐Ingo Flügge1, Frank Ludewig1
1Botanical Institute, University of Cologne, Gyrhofstr. 15, D-50931 Cologne, Germany
2Molecular Plant Physiology, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, D-06466 Gatersleben, Germany
3Plant Physiology, University of Kaiserslautern, D-67653 Kaiserslautern, Germany

Tóm tắt

SummaryTransgenic potato (Solanum tuberosum) plants simultaneously over‐expressing a pea (Pisum sativum) glucose‐6‐phosphate/phosphate translocator (GPT) and anArabidopsis thalianaadenylate translocator (NTT1) in tubers were generated. Double transformants exhibited an enhanced tuber yield of up to 19%, concomitant with an additional increased starch content of up to 28%, compared with control plants. The total starch content produced in tubers per plant was calculated to be increased by up to 44% in double transformants relative to the wild‐type. Single over‐expression of either gene had no effect on tuber starch content or tuber yield, suggesting that starch formation within amyloplasts is co‐limited by the import of energy and the supply of carbon skeletons. As total adenosine diphosphate‐glucose pyrophosphorylase and starch synthase activities remained unchanged in double transformants relative to the wild‐type, they cannot account for the increased starch content found in tubers of double transformants. Rather, an optimized supply of amyloplasts with adenosine triphosphate and glucose‐6‐phosphate seems to favour increased starch synthesis, resulting in plants with increased starch content and yield of tubers.

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Wiley

Tập 6 Số 5

453-464

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