Overexpression, purification, and characterization of recombinant Ca-ATPase regulators for high-resolution solution and solid-state NMR studies

Protein Expression and Purification - Tập 30 - Trang 253-261 - 2003
Bethany Buck1, Jamillah Zamoon2, Tara L Kirby3, Tara M DeSilva1, Christine Karim3, David Thomas3, Gianluigi Veglia1
1Department of Chemistry, University of Minnesota, 207 Pleasant ST S.E., Minneapolis, MN 55455, USA
2Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
3Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA

Tài liệu tham khảo

East, 2000, Sarco(endo)plasmic reticulum calcium pumps: recent advances in our understanding of structure/function and biology (review), Mol. Membr. Biol., 17, 189, 10.1080/09687680010009646 Schmidt, 2001, Phospholamban: a promising therapeutic target in heart failure?, Cardiovasc. Drugs Ther., 15, 387, 10.1023/A:1013381204658 MacLennan, 2000, Ca2+ signaling and muscle disease, Eur. J. Biochem., 267, 5291, 10.1046/j.1432-1327.2000.01566.x Karim, 2000, Synthetic null-cysteine phospholamban analogue and the corresponding transmembrane domain inhibit the Ca-ATPase, Biochemistry, 39, 10892, 10.1021/bi0003543 Hellstern, 2001, Sarcolipin, the shorter homologue of phospholamban, forms oligomeric structures in detergent micelles and in liposomes, J. Biol. Chem., 276, 30845, 10.1074/jbc.M102495200 Brent, 1997, Protein expression, 16.0.5 Uhlen, 1992, Fusion proteins in biotechnology, Curr. Opin. Biotechnol., 3, 363, 10.1016/0958-1669(92)90164-E Miroux, 1996, Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels, J. Mol. Biol., 260, 289, 10.1006/jmbi.1996.0399 Harrison, 2000, Expression and soluble heterologous proteins via fusion with Nusa protein, Innovations, 11, 4 de Vrij, 1986, Purification, enzymatic properties, and reconstitution of cytochrome-c oxidase from Bacillus subtilis, Methods Enzymol., 126, 159, 10.1016/S0076-6879(86)26018-8 Kimura, 1997, Phospholamban inhibitory function is activated by depolymerization, J. Biol. Chem., 272, 15061, 10.1074/jbc.272.24.15061 Uhlen, 1990, Gene fusions for purpose of expression: an introduction, Methods Enzymol., 185, 129, 10.1016/0076-6879(90)85014-F Chen, 1996, Overexpression of bacterio-opsin in Escherichia coli as a water-soluble fusion to maltose binding protein: efficient regeneration of the fusion protein and selective cleavage with trypsin, Protein Sci., 5, 456, 10.1002/pro.5560050307 Nilsson, 1997, Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins, Protein Expr. Purif., 11, 1, 10.1006/prep.1997.0767 N.A. Lockwood, R.S. Tu, Z. Zhang, M.V. Tirrell, D.D. Thomas, C.B. Karim, Structure and function of integral membrane protein domains resolved by peptide-amphiphiles: application to phospholamban, Biopolymers (2003), in press Reddy, 1999, Co-reconstitution of phospholamban mutants with the Ca-ATPase reveals dependence of inhibitory function on phospholamban structure, J. Biol. Chem., 274, 7649, 10.1074/jbc.274.12.7649 Karim, 2001, Role of cysteine residues in structural stability and function of a transmembrane helix bundle, J. Biol. Chem, 276, 38814, 10.1074/jbc.M104006200 Madden, 1979, Cholesterol modulates activity of calcium-dependent ATPase of the sarcoplasmic reticulum, Nature, 279, 538, 10.1038/279538a0 Delaglio, 1995, Nmrpipe: a multidimensional spectral processing system based on unix pipes, J. Biomol. NMR, 6, 277, 10.1007/BF00197809 Fujii, 1989, Expression and site-specific mutagenesis of phospholamban. Studies of residues involved in phosphorylation and pentamer formation, J. Biol. Chem, 264, 12950, 10.1016/S0021-9258(18)51579-9 Opella, 2001, Nuclear magnetic resonance of membrane-associated peptides and proteins, Methods Enzymol., 339, 285, 10.1016/S0076-6879(01)39319-9 McDonnell, 1993, Fd coat protein structure in membrane environments, J. Mol. Biol., 233, 447, 10.1006/jmbi.1993.1523 Reddy, 1995, Functional reconstitution of recombinant phospholamban with rabbit skeletal Ca(2+)-ATPase, J. Biol. Chem., 270, 9390, 10.1074/jbc.270.16.9390 Yao, 1996, Purification of porcine phospholamban expressed in Escherichia coli, Protein Expr. Purif., 8, 463, 10.1006/prep.1996.0125