Overexpression, purification, and characterization of recombinant Ca-ATPase regulators for high-resolution solution and solid-state NMR studies
Tài liệu tham khảo
East, 2000, Sarco(endo)plasmic reticulum calcium pumps: recent advances in our understanding of structure/function and biology (review), Mol. Membr. Biol., 17, 189, 10.1080/09687680010009646
Schmidt, 2001, Phospholamban: a promising therapeutic target in heart failure?, Cardiovasc. Drugs Ther., 15, 387, 10.1023/A:1013381204658
MacLennan, 2000, Ca2+ signaling and muscle disease, Eur. J. Biochem., 267, 5291, 10.1046/j.1432-1327.2000.01566.x
Karim, 2000, Synthetic null-cysteine phospholamban analogue and the corresponding transmembrane domain inhibit the Ca-ATPase, Biochemistry, 39, 10892, 10.1021/bi0003543
Hellstern, 2001, Sarcolipin, the shorter homologue of phospholamban, forms oligomeric structures in detergent micelles and in liposomes, J. Biol. Chem., 276, 30845, 10.1074/jbc.M102495200
Brent, 1997, Protein expression, 16.0.5
Uhlen, 1992, Fusion proteins in biotechnology, Curr. Opin. Biotechnol., 3, 363, 10.1016/0958-1669(92)90164-E
Miroux, 1996, Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels, J. Mol. Biol., 260, 289, 10.1006/jmbi.1996.0399
Harrison, 2000, Expression and soluble heterologous proteins via fusion with Nusa protein, Innovations, 11, 4
de Vrij, 1986, Purification, enzymatic properties, and reconstitution of cytochrome-c oxidase from Bacillus subtilis, Methods Enzymol., 126, 159, 10.1016/S0076-6879(86)26018-8
Kimura, 1997, Phospholamban inhibitory function is activated by depolymerization, J. Biol. Chem., 272, 15061, 10.1074/jbc.272.24.15061
Uhlen, 1990, Gene fusions for purpose of expression: an introduction, Methods Enzymol., 185, 129, 10.1016/0076-6879(90)85014-F
Chen, 1996, Overexpression of bacterio-opsin in Escherichia coli as a water-soluble fusion to maltose binding protein: efficient regeneration of the fusion protein and selective cleavage with trypsin, Protein Sci., 5, 456, 10.1002/pro.5560050307
Nilsson, 1997, Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins, Protein Expr. Purif., 11, 1, 10.1006/prep.1997.0767
N.A. Lockwood, R.S. Tu, Z. Zhang, M.V. Tirrell, D.D. Thomas, C.B. Karim, Structure and function of integral membrane protein domains resolved by peptide-amphiphiles: application to phospholamban, Biopolymers (2003), in press
Reddy, 1999, Co-reconstitution of phospholamban mutants with the Ca-ATPase reveals dependence of inhibitory function on phospholamban structure, J. Biol. Chem., 274, 7649, 10.1074/jbc.274.12.7649
Karim, 2001, Role of cysteine residues in structural stability and function of a transmembrane helix bundle, J. Biol. Chem, 276, 38814, 10.1074/jbc.M104006200
Madden, 1979, Cholesterol modulates activity of calcium-dependent ATPase of the sarcoplasmic reticulum, Nature, 279, 538, 10.1038/279538a0
Delaglio, 1995, Nmrpipe: a multidimensional spectral processing system based on unix pipes, J. Biomol. NMR, 6, 277, 10.1007/BF00197809
Fujii, 1989, Expression and site-specific mutagenesis of phospholamban. Studies of residues involved in phosphorylation and pentamer formation, J. Biol. Chem, 264, 12950, 10.1016/S0021-9258(18)51579-9
Opella, 2001, Nuclear magnetic resonance of membrane-associated peptides and proteins, Methods Enzymol., 339, 285, 10.1016/S0076-6879(01)39319-9
McDonnell, 1993, Fd coat protein structure in membrane environments, J. Mol. Biol., 233, 447, 10.1006/jmbi.1993.1523
Reddy, 1995, Functional reconstitution of recombinant phospholamban with rabbit skeletal Ca(2+)-ATPase, J. Biol. Chem., 270, 9390, 10.1074/jbc.270.16.9390
Yao, 1996, Purification of porcine phospholamban expressed in Escherichia coli, Protein Expr. Purif., 8, 463, 10.1006/prep.1996.0125
