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Biểu hiện quá mức của gen vận chuyển photphat từ cây lúa OsPT2 làm tăng cường khả năng cố định nitơ và đồng hoá amoniac ở đậu nành chuyển gen trong điều kiện thiếu photpho
Tóm tắt
Thiếu photpho (P) là một trong những yếu tố chính hạn chế sự nốt tạo và cố định nitơ (N) ở cây đậu, từ đó ảnh hưởng đến năng suất của cây đậu. Trong nghiên cứu trước đây, chúng tôi đã chỉ ra rằng ba dòng đậu nành chuyển gen T2 biểu hiện quá mức gen vận chuyển photphat từ cây lúa OsPT2 cho thấy khả năng chịu stress P thấp tốt hơn. Nghiên cứu này nhằm mục đích xác định xem việc biểu hiện quá mức OsPT2 có làm tăng khả năng cố định N2 và đồng hoá amoniac ở ba dòng chuyển gen đồng hợp T3 (HTLs) trong điều kiện thiếu P hay không. Dưới điều kiện photphat vô cơ (Pi) thấp, nồng độ P, tổng N và tổng ureide trong các dòng T3 HTLs cao hơn đáng kể so với cây kiểu hoang dã (WT). Hơn nữa, các dòng T3 HTLs cho thấy hiệu suất tăng trưởng cây và phát triển nốt tốt hơn đáng kể so với cây WT trong điều kiện thấp-Pi. Phân tích PCR thời gian thực định lượng (qRT-PCR) cho thấy mức độ biểu hiện của GmENOD40-1, GmENOD40-2 (hai gen nốt tạo sớm), và GmLba (một gen leghemoglobin) tăng đáng kể ở các dòng T3 HTLs trong điều kiện thiếu P vào 24 và 32 ngày sau khi inoculation (DAI). Các mức độ transcript được tăng lên của GmGS1β1 và GmGS1β2 (hai gen glutamine synthetase tế bào chất) ở các dòng T3 HTLs phù hợp với sự gia tăng hoạt động glutamine synthetase (GS, EC 6.3.1.2) vào 32 DAI. Kết quả của chúng tôi cho thấy rằng việc biểu hiện quá mức OsPT2 trong các dòng T3 HTLs làm tăng cường khả năng cố định N2 và hoạt động đồng hoá amoniac trong điều kiện stress P thấp.
Từ khóa
#photpho #cố định nitơ #đồng hoá amoniac #đậu nành chuyển gen #gen OsPT2Tài liệu tham khảo
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