Overexpression of lncRNA AFAP1-AS1 as a diagnostic biomarker in non-small cell lung cancer

Egyptian Journal of Medical Human Genetics - Tập 22 - Trang 1-5 - 2021
Sajjad Ghalib Ibrahim Alnajar1, Ali Rajabi1, Melika Maydanchi2, Samaneh Tayefeh Gholami1, Ali Saber2, Reza Safaralizadeh1
1Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
2Zimagene Medical Genetics Laboratory, Hamedan, Iran

Tóm tắt

Long non-coding RNAs (lncRNAs) play important roles in lung tumorigenesis. Among different lncRNAs, overexpression of the lncRNA actin filament‐associated protein 1‐antisense RNA 1 (AFAP1-AS1) in lung tumors has been reported in different studies. In the current study, we aimed to investigate the potential value of lncRNA AFAP1-AS1 as a diagnostic biomarker in lung cancer. Ninety samples from patients with lung cancer were collected from Noor-E-Nejat hospital, Tabriz, Iran. The expression of AFAP1-AS1 was assessed using quantitative reverse transcriptase-PCR (qRT-PCR), followed by the ROC curve analysis to investigate the biomarker potency of AFAP1-AS1. Our results revealed an upregulation of AFAP1-AS1 in tumor samples as compared to the adjacent non-tumor tissues. We found a significant positive association between AFAP1-AS1 expression and tumor size, as well as tumor stage. Our results showed overexpression of AFAP1-AS1 and its capacity as a diagnostic biomarker in lung cancer.

Tài liệu tham khảo

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