Biểu hiện quá mức của OsHAD3, một thành viên của siêu họ HAD, làm giảm khả năng chịu hạn của cây lúa

Rice - 2023
Xiaofei Zan1, Zhanmei Zhou1, Jin Wan1, Xuejiao Chen1, Jiali Zhu1, Hongyan Xu1, Jia Zhang1, Xiaohong Li1, Xiaoling Gao1, Rongjun Chen1, Zhengjian Huang1, Zhengjun Xu1, Lihua Li1
1Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, People’s Republic of China

Tóm tắt

Tóm tắtSiêu họ hydrolase giống haloacid dehalogenase (HAD) đã được chứng minh có vai trò trong sự phát triển cây trồng và phản ứng với căng thẳng abiotic. Mặc dù các chức năng và cơ chế điều chỉnh khác nhau của siêu họ HAD đã được chứng minh rõ ràng, nhưng chúng ta biết rất ít về chức năng của gia đình này trong việc cải thiện khả năng chịu căng thẳng abiotic cho cây lúa. Tại đây, chúng tôi báo cáo rằng OsHAD3, một thành viên của siêu họ HAD, có thể ảnh hưởng đến khả năng chịu hạn của cây lúa. Dưới căng thẳng hạn, sự biểu hiện quá mức của OsHAD3 làm tăng tích tụ các loại oxy phản ứng và malondialdehyde so với loại hoang dã. Các dòng biểu hiện quá mức OsHAD3 giảm chiều dài rễ nhưng các dòng biểu hiện đối kháng lại tăng chiều dài rễ dưới căng thẳng hạn, và mức độ phiên mã của nhiều gen liên quan đến căng thẳng nổi tiếng cũng đã thay đổi trong các cây trồng có kiểu gen khác nhau. Hơn nữa, việc biểu hiện quá mức của OsHAD3 cũng làm giảm khả năng chịu oxi hóa. Kết quả của chúng tôi gợi ý rằng biểu hiện quá mức của OsHAD3 có thể làm giảm khả năng chịu hạn của cây lúa và cung cấp một chiến lược mới để cải thiện khả năng chịu hạn ở cây lúa.

Từ khóa


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