Thành phần Vi khuẩn Miệng Phản ánh Nguy Cơ Tiềm Tàng Đối Với Các Loại Ung Thư Thực Quản

American Association for Cancer Research (AACR) - Tập 77 Số 23 - Trang 6777-6787 - 2017
Brandilyn A. Peters1, Jing Wu1,2, Zhiheng Pei2,3,4, Liying Yang5, Mark P. Purdue6, Neal D. Freedman6, Eric J. Jacobs7, Susan M. Gapstur7, Richard B. Hayes1,2, Jiyoung Ahn1,2
11Division of Epidemiology, Department of Population Health, NYU School of Medicine, New York, New York.
22NYU Perlmutter Cancer Center, New York, New York.
33Department of Pathology, NYU School of Medicine, New York, New York.
44Department of Veterans Affairs New York Harbor Healthcare System, New York, New York.
55Division of Translational Medicine, Department of Medicine, NYU School of Medicine, New York, New York.
66Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, Maryland.
77Epidemiology Research Program, American Cancer Society, Atlanta, Georgia.

Tóm tắt

Tóm tắt Các vi khuẩn có thể đóng vai trò trong ung thư biểu mô tuyến thực quản (EAC) và ung thư tế bào vảy thực quản (ESCC), mặc dù bằng chứng hiện tại chủ yếu dựa vào các nghiên cứu cắt ngang. Trong nghiên cứu này, chúng tôi đã xem xét mối quan hệ giữa hệ vi sinh vật miệng và nguy cơ EAC cùng với ESCC trong một nghiên cứu tiên tiến tích hợp trong hai nhóm. Vi khuẩn miệng được đánh giá bằng phương pháp giải trình tự gene 16S rRNA từ các mẫu nước súc miệng tiền chẩn đoán từ n = 81/160 trường hợp EAC và n = 25/50 trường hợp ESCC cùng nhóm đối chứng. Các phát hiện chủ yếu nhất quán giữa hai nhóm. Nội dung metagenome được dự đoán bằng cách sử dụng PiCRUST. Chúng tôi đã xem xét các mối liên hệ giữa sự phong phú của taxon hoặc con đường chức năng được chuyển đổi theo tỷ lệ log trung tâm và nguy cơ bằng cách sử dụng hồi quy logistic có điều kiện, điều chỉnh cho chỉ số khối cơ thể (BMI), thuốc lá và rượu. Chúng tôi phát hiện ra rằng tác nhân gây bệnh ở nướu răng Tannerella forsythia liên quan đến nguy cơ cao hơn của EAC. Hơn nữa, sự suy giảm của giống vi khuẩn cộng sinh Neisseria và loài Streptococcus pneumoniae được liên kết với nguy cơ EAC thấp hơn. Sinh tổng hợp vi khuẩn carotenoid cũng có liên quan đến sự bảo vệ chống lại EAC. Cuối cùng, sự phong phú của tác nhân gây bệnh ở nướu Porphyromonas gingivalis có xu hướng liên quan đến nguy cơ cao hơn của ESCC. Tổng thể, những phát hiện của chúng tôi có thể có ý nghĩa trong việc phát hiện sớm và phòng ngừa EAC cũng như ESCC. Cancer Res; 77(23); 6777–87. ©2017 AACR.

Từ khóa


Tài liệu tham khảo

Ferlay, 2015, Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012, Int J Cancer, 136, E359, 10.1002/ijc.29210

Gupta, 2017, Worldwide incidence, mortality and time trends for cancer of the oesophagus, Eur J Cancer Prev, 26, 107, 10.1097/CEJ.0000000000000249

Rubenstein, 2015, Epidemiology, diagnosis, and management of esophageal adenocarcinoma, Gastroenterology, 149, 302, 10.1053/j.gastro.2015.04.053

Engel, 2003, Population attributable risks of esophageal and gastric cancers, J Natl Cancer Inst, 95, 1404, 10.1093/jnci/djg047

Yang, 2014, Microbiome in reflux disorders and esophageal adenocarcinoma, Cancer J, 20, 207, 10.1097/PPO.0000000000000044

Kauppila, 2014, Toll-like receptors in esophageal cancer, Front Immunol, 5, 200, 10.3389/fimmu.2014.00200

Blackett, 2013, Oesophageal bacterial biofilm changes in gastro-oesophageal reflux disease, Barrett's and oesophageal carcinoma: association or causality?, Aliment Pharmacol Ther, 37, 1084, 10.1111/apt.12317

Yang, 2009, Inflammation and intestinal metaplasia of the distal esophagus are associated with alterations in the microbiome, Gastroenterology, 137, 588, 10.1053/j.gastro.2009.04.046

Liu, 2013, Characterization of bacterial biota in the distal esophagus of Japanese patients with reflux esophagitis and Barrett's esophagus, BMC Infect Dis, 13, 130, 10.1186/1471-2334-13-130

Macfarlane, 2007, Microbial colonization of the upper gastrointestinal tract in patients with Barrett's esophagus, Clin Infect Dis, 45, 29, 10.1086/518578

Zaidi, 2015, Associations of microbiota and toll-like receptor signaling pathway in esophageal adenocarcinoma, BMC Cancer, 16, 52, 10.1186/s12885-016-2093-8

Yu, 2014, Association between upper digestive tract microbiota and cancer-predisposing states in the esophagus and stomach, Cancer Epidemiol Biomarkers Prev, 23, 735, 10.1158/1055-9965.EPI-13-0855

Chen, 2015, Oral microbiota and risk for esophageal squamous cell carcinoma in a high-risk area of China, PLoS One, 10, e0143603, 10.1371/journal.pone.0143603

Michaud, 2016, Periodontal disease and risk of all cancers among male never smokers: an updated analysis of the Health Professionals Follow-up Study, Ann Oncol, 27, 941, 10.1093/annonc/mdw028

Pei, 2004, Bacterial biota in the human distal esophagus, Proc Natl Acad Sci U S A, 101, 4250, 10.1073/pnas.0306398101

Lawson, 2010, The noncolonic microbiome: does it really matter?, Curr Gastroenterol Rep, 12, 259, 10.1007/s11894-010-0111-6

Hayes, 2000, Etiologic and early marker studies in the prostate, lung, colorectal and ovarian (PLCO) cancer screening trial, Control Clin Trials, 21, 349S, 10.1016/S0197-2456(00)00101-X

Calle, 2002, The American Cancer Society Cancer Prevention Study II Nutrition Cohort: rationale, study design, and baseline characteristics, Cancer, 94, 2490, 10.1002/cncr.101970

U.S. Department of Agriculture, 2010, Dietary Guidelines for Americans, 2010

Belstrom, 2016, Temporal stability of the salivary microbiota in oral health, PLoS One, 11, e0147472, 10.1371/journal.pone.0147472

Zhou, 2013, Biogeography of the ecosystems of the healthy human body, Genome Biol, 14, R1, 10.1186/gb-2013-14-1-r1

Costello, 2009, Bacterial community variation in human body habitats across space and time, Science, 326, 1694, 10.1126/science.1177486

Caporaso, 2010, QIIME allows analysis of high-throughput community sequencing data, Nat Methods, 7, 335, 10.1038/nmeth.f.303

Chen, 2010, The Human Oral Microbiome Database: a web accessible resource for investigating oral microbe taxonomic and genomic information, Database, 2010, baq013, 10.1093/database/baq013

Price, 2009, FastTree: computing large minimum evolution trees with profiles instead of a distance matrix, Mol Biol Evol, 26, 1641, 10.1093/molbev/msp077

Lozupone, 2007, Quantitative and qualitative beta diversity measures lead to different insights into factors that structure microbial communities, Appl Environmental Microbiol, 73, 1576, 10.1128/AEM.01996-06

van Buuren, 2011, mice: multivariate imputation by chained equations., R. J Stat Softw, 45, 1

McArdle, 2001, Fitting multivariate models to community data: a comment on distance-based redundancy analysis, Ecology, 82, 290, 10.1890/0012-9658(2001)082[0290:FMMTCD]2.0.CO;2

Fernandes, 2014, Unifying the analysis of high-throughput sequencing datasets: characterizing RNA-seq, 16S rRNA gene sequencing and selective growth experiments by compositional data analysis, Microbiome, 2, 15, 10.1186/2049-2618-2-15

Socransky, 1998, Microbial complexes in subgingival plaque, J Clin Periodontol, 25, 134, 10.1111/j.1600-051X.1998.tb02419.x

Langille, 2013, Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences, Nat Biotechnol, 31, 814, 10.1038/nbt.2676

Kanehisa, 2012, KEGG for integration and interpretation of large-scale molecular data sets, Nucleic Acids Res, 40, D109, 10.1093/nar/gkr988

Kurtz, 2015, Sparse and compositionally robust inference of microbial ecological networks, PLoS Comput Biol, 11, e1004226, 10.1371/journal.pcbi.1004226

Dabu, 2012, Identification of four Treponema species in subgingival samples by nested-PCR and their correlation with clinical diagnosis, Roum Arch Microbiol Immunol, 71, 43

Willis, 1999, Identification of seven Treponema species in health- and disease-associated dental plaque by nested PCR, J Clin Microbiol, 37, 867, 10.1128/JCM.37.3.867-869.1999

Hiraki, 2008, Teeth loss and risk of cancer at 14 common sites in Japanese, Cancer Epidemiol Biomarkers Prev, 17, 1222, 10.1158/1055-9965.EPI-07-2761

Abnet, 2001, Prospective study of tooth loss and incident esophageal and gastric cancers in China, Cancer Causes Control, 12, 847, 10.1023/A:1012290009545

Guha, 2007, Oral health and risk of squamous cell carcinoma of the head and neck and esophagus: results of two multicentric case-control studies, Am J Epidemiol, 166, 1159, 10.1093/aje/kwm193

Gao, 2016, Presence of Porphyromonas gingivalis in esophagus and its association with the clinicopathological characteristics and survival in patients with esophageal cancer, Infect Agent Cancer, 11, 3, 10.1186/s13027-016-0049-x

Liu, 2015, Non-pathogenic Neisseria: members of an abundant, multi-habitat, diverse genus, Microbiology, 161, 1297, 10.1099/mic.0.000086

Wu, 2016, Cigarette smoking and the oral microbiome in a large study of American adults, ISME J, 10, 2435, 10.1038/ismej.2016.37

Morris, 2013, Comparison of the respiratory microbiome in healthy nonsmokers and smokers, Am J Respir Crit Care Med, 187, 1067, 10.1164/rccm.201210-1913OC

Colman, 1976, Cigarette smoking and the microbial flora of the mouth, Aust Dent J, 21, 111, 10.1111/j.1834-7819.1976.tb02833.x

Liu, 2013, Health-promoting components of fruits and vegetables in the diet, Adv Nutr, 4, 384s, 10.3945/an.112.003517

Kubo, 2007, Meta-analysis of antioxidant intake and the risk of esophageal and gastric cardia adenocarcinoma, Am J Gastroenterol, 102, 2323, 10.1111/j.1572-0241.2007.01374.x

Dutta, 2012, Barrett's esophagus and beta-carotene therapy: symptomatic improvement in GERD and enhanced HSP70 expression in esophageal mucosa, Asian Pac J Cancer Prev, 13, 6011, 10.7314/APJCP.2012.13.12.6011

Huang, 2003, Iron overload and its association with cancer risk in humans: evidence for iron as a carcinogenic metal, Mutat Res, 533, 153, 10.1016/j.mrfmmm.2003.08.023

Keeler, 2013, Iron chelation: a potential therapeutic strategy in oesophageal cancer, Br J Pharmacol, 168, 1313, 10.1111/bph.12093

Ganz, 2015, Iron homeostasis in host defence and inflammation, Nat Rev Immunol, 15, 500, 10.1038/nri3863

Freedberg, 2014, The impact of proton pump inhibitors on the human gastrointestinal microbiome, Clin Lab Med, 34, 771, 10.1016/j.cll.2014.08.008

Abeles, 2016, Microbial diversity in individuals and their household contacts following typical antibiotic courses, Microbiome, 4, 39, 10.1186/s40168-016-0187-9

Thông tin
Thông tin xuất bản

American Association for Cancer Research (AACR)

Tập 77 Số 23

6777-6787

Thông tin tác giả