Oral Administration of Skin Gelatin Isolated from Chum Salmon (Oncorhynchus keta) Enhances Wound Healing in Diabetic Rats

Marine Drugs - Tập 9 Số 5 - Trang 696-711
Zhaofeng Zhang1, Ming Zhao1,2, Junbo Wang1, Ye Ding1, Xiaoqian Dai1, Yong Li1
1Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China
2Integrative Biology and Physiology

Tóm tắt

Care for diabetic wounds remains a significant clinical problem. The present study was aimed at investigating the effect of skin gelatin from Chum Salmon on defective wound repair in the skin of diabetic rats. Full-thickness excisional skin wounds were made in 48 rats, of which 32 were diabetes. The diabetic rats were orally treated daily for 14 days with skin gelatin from Chum Salmon (2 g/kg) or its vehicle. Sixteen non-diabetic control rats received the same amount of water as vehicle-treated non-diabetic rats. Rats were killed to assess the rate of wound closure, microvessel density (MVD), vascular endothelial growth factor (VEGF), hydroxyproline (HP) contents in wound tissues and nitrate in plasma and wound tissue at 7 and 14 days after wounding. Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hyproxyproline and NO contents and a reduced extent of inflammatory response. All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group. In light of our finding that skin gelatin of Chum Salmon promotes skin wound repair in diabetic rats, we propose that oral administration of Chum Salmon skin gelatin might be a beneficial method for treating wound disorders associated with diabetes.

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Tài liệu tham khảo

Greenhalgh, 2003, Wound healing and diabetes mellitus, Clin Plast Surg, 30, 37, 10.1016/S0094-1298(02)00066-4

Stadelmann, 1998, Impediments to wound healing, Am J Surg, 176, 39S, 10.1016/S0002-9610(98)00184-6

Galeano, 2008, Polydeoxyribonucleotide stimulates angiogenesis and wound healing in the genetically diabetic mouse, Wound Repair Regen, 16, 208, 10.1111/j.1524-475X.2008.00361.x

Han, 2009, Effects of cod bone gelatin on bone metabolism and bone microarchitecture in ovariectomized rats, Bone, 44, 942, 10.1016/j.bone.2008.12.005

Lee, 2003, Bio-artificial skin composed of gelatin and (1→3), (1→6)-beta-glucan, Biomaterials, 24, 2503, 10.1016/S0142-9612(03)00003-6

Wang, 2006, The effect of gelatin-chondroitin sulfate-hyaluronic acid skin substitute on wound healing in SCID mice, Biomaterials, 27, 5689, 10.1016/j.biomaterials.2006.07.024

Kawai, 2005, Accelerated wound healing through the incorporation of basic fibroblast growth factor-impregnated gelatin microspheres into artificial dermis using a pressure-induced decubitus ulcer model in genetically diabetic mice, Br J Plast Surg, 58, 1115, 10.1016/j.bjps.2005.04.010

Thilagar, 2009, Effect of keratin-gelatin and bFGF-gelatin composite film as a sandwich layer for full-thickness skin mesh graft in experimental dogs, J Biomed Mater Res B Appl Biomater, 88, 12, 10.1002/jbm.b.31024

Hori, 2007, Controlled-release of epidermal growth factor from cationized gelatin hydrogel enhances corneal epithelial wound healing, J Control Release, 118, 169, 10.1016/j.jconrel.2006.12.011

Li, 2007, Pathophysiology of acute wound healing, Clin Dermatol, 25, 9, 10.1016/j.clindermatol.2006.09.007

Brem, 2007, Cellular and molecular basis of wound healing in diabetes, J Clin Invest, 117, 1219, 10.1172/JCI32169

Stadelmann, 1998, Physiology and healing dynamics of chronic cutaneous wounds, Am J Surg, 176, 26S, 10.1016/S0002-9610(98)00183-4

Schwentker, 2002, Nitric oxide and wound repair: Role of cytokines, Nitric Oxide, 7, 1, 10.1016/S1089-8603(02)00002-2

Witte, 2002, Nitric oxide enhances experimental wound healing in diabetes, Brit J Surg, 89, 1594, 10.1046/j.1365-2168.2002.02263.x

Blakytny, 2006, The molecular biology of chronic wounds and delayed healing in diabetes, Diabet Med, 23, 594, 10.1111/j.1464-5491.2006.01773.x

Moulin, 2000, Role of wound healing myofibroblasts on re-epithelialization of human skin, Burns, 26, 3, 10.1016/S0305-4179(99)00091-1

Martin, 2003, Abnormal angiogenesis in diabetes mellitus, Med Res Rev, 23, 117, 10.1002/med.10024

Roy, 2006, Biology of vascular endothelial growth factors, FEBS Lett, 580, 2879, 10.1016/j.febslet.2006.03.087

Bitto, 2008, Simvastatin enhances VEGF production and ameliorates impaired wound healing in experimental diabetes, Pharmacol Res, 57, 159, 10.1016/j.phrs.2008.01.005

Cha, 2008, Fibroblasts from non-healing human chronic wounds show decreased expression of beta ig-h3, a TGF-beta inducible protein, J Dermatol Sci, 50, 15, 10.1016/j.jdermsci.2007.10.010

Santos, 2007, VEGF and VEGFR-2 (KDR) internalization is required for endothelial recovery during wound healing, Exp Cell Res, 313, 1561, 10.1016/j.yexcr.2007.02.020

Galiano, 2004, Topical vascular endothelial growth factor accelerates diabetic wound healing through increased angiogenesis and by mobilizing and recruiting bone marrow-derived cells, Am J Pathol, 164, 1935, 10.1016/S0002-9440(10)63754-6

Bitto, 2008, Angiopoietin-1 gene transfer improves impaired wound healing in genetically diabetic mice without increasing VEGF expression, Clin Sci (Lond), 114, 707, 10.1042/CS20070250

Nascimento, 2009, Propranolol improves cutaneous wound healing in streptozotocin-induced diabetic rats, Eur J Pharmacol, 611, 77, 10.1016/j.ejphar.2009.03.053

Senel, 1997, Oxygen free radicals impair wound healing in ischemic rat skin, Ann Plast Surg, 39, 516, 10.1097/00000637-199711000-00012

Pei, 2009, Marine collagen peptide isolated from Chum Salmon (Oncorhynchus keta) skin facilitates learning and memory in aged C57BL/6J mice, Food Chem, 118, 333, 10.1016/j.foodchem.2009.04.120

Kim, 2001, Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska pollack skin, J Agric Food Chem, 49, 1984, 10.1021/jf000494j

Khantaphant, 2008, Comparative study on the proteases from fish pyloric caeca and the use for production of gelatin hydrolysate with antioxidative activity, Comp Biochem Physiol B Biochem Mol Biol, 151, 410, 10.1016/j.cbpb.2008.08.011

Songchotikunpan, 2008, Extraction and electrospinning of gelatin from fish skin, Int J Biol Macromol, 42, 247, 10.1016/j.ijbiomac.2007.11.005

Taheri, 2009, Extraction and physicochemical characterization of greater Lizardfish (Saurida tumbil) skin and bone gelatin, J Food Sci, 74, E160, 10.1111/j.1750-3841.2009.01106.x

Li, 2005, Kinetic expression of platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) during embryonic stem cell differentiation, J Cell Biochem, 95, 559, 10.1002/jcb.20436

Stark, 1992, Detection of enhanced neutrophil adhesion to parainfluenza-infected airway epithelial cells using a modified myeloperoxidase assay in a microtiter format, J Virol Methods, 40, 225, 10.1016/0166-0934(92)90071-K