Optimization of the In-Silico-Designed Kemp Eliminase KE70 by Computational Design and Directed Evolution

Journal of Molecular Biology - Tập 407 - Trang 391-412 - 2011
Olga Khersonsky1, Daniela Röthlisberger2, Andrew M. Wollacott2, Paul Murphy2, Orly Dym3, Shira Albeck3, Gert Kiss4, K.N. Houk4, David Baker2,5,6, Dan S. Tawfik1
1Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
2Department of Biochemistry, University of Washington, Seattle, WA 98195 USA
3Israel Structural Proteomics Center, Weizmann Institute of Science, Rehovot 76100, Israel
4Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
5Biomolecular Structure and Design, University of Washington, Seattle, WA 98195, USA
6Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195 USA

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