Optimization of intracerebroventricular streptozotocin dose for the induction of neuroinflammation and memory impairments in rats

Metabolic Brain Disease - Tập 35 - Trang 1279-1286 - 2020
Rupsa Ghosh1, Susmita Sil1,2, Pritha Gupta1, Tusharkanti Ghosh1
1Neurophysiology Laboratory, Department of Physiology, University College of Science and Technology, University of Calcutta, Kolkata, India
2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, USA

Tóm tắt

Intracerebroventricular (ICV) injection of streptozotocin (STZ) is a well established procedure to induce neuroinflammation leading to dementia in experimental animals. However, the optimal dose of STZ has not been determined. In the present study, rats were ICV injected with 1.5, 3 and 6 mg of STZ per kg of body weight. After 21 days, neuroinflammatory markers i.e. TNF-α, IL-1β, ROS and nitrite were quantified in the hippocampus. Memory function was assessed by the radial arm maze test after 9, 12, 15, 18, 21 days following STZ injection. STZ treatment significantly increased neuroinflammatory markers and decreased memory functions in a dose dependent manner showing optimum effects at the dose of 3 mg/kg.

Tài liệu tham khảo

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