Optimization of an AC electrokinetics immunoassay lab-chip for biomedical diagnostics
Tóm tắt
This paper describes the improvement of an immunoassay lab-chip based on AC electrokinetics (ACEK) effects. Reagent and analyte delivery, incubation and flushing are performed using AC electrothermal (ACET) micropumps, which operate by applying low-voltage AC signals over interdigitated electrode arrays on the microchannel bottom. As such, the immunoassay chip is low cost and disposable. Furthermore, ACET flow will generate vortices above the electrodes, which helps to transport biomolecules towards the binding sites. Concomitant ACEK effects, especially dielectrophoresis (DEP), create enhanced trapping, however, lead to severe nonspecific response. The work in this paper involves improving the binding process in a multifunctional microfluidic system. Our experiments observed a 30-fold acceleration in detection with actual serum samples, i.e., the incubation time is shortened from 30 min to around 1 min, while achieving differentiation between the disease positive and negative sera.
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