Optimization of Carbon Dioxide and Valeric Acid Utilization for Polyhydroxyalkanoates Synthesis by Cupriavidus necator
Tóm tắt
The utilization of captured CO2 as a part of the CO2 capture and storage system to produce biopolymers could address current environmental issues such as global warming and depletion of resources. In this study, the effect of feeding strategies of CO2 and valeric acid on cell growth and synthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] in Cupriavidus necator was investigated to determine the optimal conditions for microbial growth and biopolymer accumulation. Among the studied CO2 concentrations (1–20 %), microbial growth and poly(3-hydroxybutyrate) accumulation were optimal at 1 % CO2 using a gas mixture at H2:O2:N2 = 7:1:91 % (v/v). When valeric acid was fed together with 1 % CO2, (R)-3-hydroxyvalerate synthesis increased with increasing valeric acid concentration up to 0.1 %, but (R)-3-hydroxybutyrate synthesis was inhibited at >0.05 % valeric acid. Sequential addition of valeric acid (0.05 % at Day 0 followed by 0.025 % at Day 2) showed an increase in 3HV fraction without inhibitory effects on 3HB synthesis during 4 d accumulation period. The resulting P(3HB-co-3HV) with 17–32 mol % of 3HV is likely to be biocompatible. The optimal concentrations and feeding strategies of CO2 and valeric acid determined in this study for microbial P(3HB-co-3HV) synthesis can be used to produce biocompatible P(3HB-co-3HV).
Tài liệu tham khảo
Madison LL, Huisman GW (1999) Microbiol Mol Biol Rev 63:21
Verlinden RAJ, Hill DJ, Kenward MA, Willians CD, Radecka I (2007) J Appl Microbiol 102:1437
Ishizaki A, Tanaka K, Taga N (2001) Appl Microbiol Biotechnol 57:6
Khanna S, Srivastava AK (2005) Process Biochem 40:607
Hunt AJ, Sin EHK, Marriott R, Clark JH (2010) Chem Sus Chem 3:306
Akaraonye E, Keshavarz T, Roy I (2010) J Chem Technol Biotechnol 85:732
Chen GQ, Wu Q (2005) Biomaterials 26:6565
Khanna S, Srivastava AK (2007) J Ind Microbiol Biotechnol 34:457
Anderson AJ, Dawers EA (1990) Microbiol Rev 54:450
Ishizaki A, Tanaka K (1991) J Ferment Bioeng 71:254
Tanaka K, Ishizaki A (1995) Biotechnol Bioeng 45:268
Tanaka K, Ishizaki A (1994) J Ferment Bioeng 77:425
Volova TG, Kalacheva GS, Gorbunova OV, Zhila NO (2004) Appl Biochem Microbiol 40:170
Volova TG, Kalacheva GS (2005) Microbiology 74:54
Volova TG, Kiselev EG, Shishatskaya EI, Zhila NO, Boyandin AN, Syrvacheva DA, Vinogradova ON, Kalacheva GS, Vasiliev AD, Peterson IV (2013) Bioresource Technol 146:215
Volova TG, Kalacheva GS, Steinbuchel A (2008) Macromol Symp 269:1
Werker A, Lind P, Bengtsson S, Nordstrom F (2008) Water Res 42:2517
Jung YK, Kim TY, Park SJ, Lee SY (2010) Biotechnol Bioeng 105:161
Gunarante L, Shanks R, Amarasinghe G (2004) Thermochim Acta 423:127
Pedros-Alio C, Mas J, Guerrero R (1985) Arch Microbiol 143:178
Byrom D (1987) Trends Biotechnol 5:246
Pohlmann A (2006) Nat Biotechnol 24:1257
Reinecke F, Steinbuchel A (2009) J Mol Microbiol Biotechnol 16:91
Bongers L (1970) J Bacteriol 104:145
Dixon NM, Kell DB (1989) J Appl Bacteriol 67:109
Shang L, Jiang M, Ryu CH, Chang HN, Cho SH, Lee JW (2003) Biotechnol Bioeng 83:312
Shang L, Yim SC, Park HG, Chang HN (2004) Biotechnol Prog 20:140
Kim BS, Lee SC, Lee SY, Chang HN, Chang YK, Woo SI (1994) Enzyme Microb Technol 16:556
Du GC, Chen J, Yu J, Lun S (2001) Biochem Eng J 8:103
Kim DY, Park DS, Kwon SB, Chung MG, Bae KS, Park HY, Rhee YH (2009) J Microbiol 47:651
Volova TG (2004) Polyhydroxyalkanoates-plastic materials of the 21st century: production, properties, applications. Nova Science Publishers, New York
Scandola M, Pizzoli M, Ceccorulli G, Cesàro A, Paolletti S, Navarini L (1988) Int Biol Macromol 10:373
Cesaro A, Scandola M (1989) Chimicaoggi 7:81
Mitomo H, Barham PJ, Keller A (1987) Polym J 19:1241
Madden LA, Anderson JA (1998) Macromolecules 31:5660
Doi Y (1990) Microbial polyesters. VCH, New York
Volova T, Shishatskaya E, Sevastianov V, Efremov S, Mogilnaya O (2003) Biochem Eng J 16:125
Shishatskaya EI, Volova TG, Puzyr AP, Mogilnaya OA (2004) J Mater Sci Mater Med 15:719