Ontogenetic development of the diencephalic MCH neurons: a hypothalamic ‘MCH area’ hypothesis

European Journal of Neuroscience - Tập 13 Số 9 - Trang 1733-1744 - 2001
Frédéric Brischoux1, D Fellmann1, Pierre‐Yves Risold1
1Laboratoire d'Histologie, Embryologie, Cytogénétique, CNRS FRE 2174, Faculté de Médecine et de Pharmacie, Université de Franche-Comté, Place St Jacques, 25030 Besançon Cedex, France

Tóm tắt

Abstract

The ontogeny of rat diencephalic melanin‐concentrating hormone (MCH) neurons has been analysed, using the bromodeoxyuridine method to determine the period of birth of these neurons, and using in situ hybridization and immunohistochemistry to study their chemical differentiation. The spatiotemporal pattern of MCH neuron generation is complex, although it is broadly lateromedial with a peak between embryonic days (E) 12 and E13. The first expression of the MCH gene has been detected on E13 in neurons in the presumptive lateral hypothalamic area. But the adult‐like pattern was observed from E18. Medial‐most MCH neurons express the peptide CART (cocaine‐amphetamine‐regulated transcript) from E18, and the receptor neurokinin 3 (NK3) from between postnatal day (P) 0 and P5. These results are discussed and compared with data from the literature to better understand the organization of the ‘MCH‐containing area’.

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Tài liệu tham khảo

10.1002/cne.901820511

10.1002/cne.901820512

Altman J., 1986, Advances in Anatomy, Embryology and Cell Biology, 1

10.1016/s0306-4522(98)00678-2

10.1002/cne.903190204

10.1002/(SICI)1096-9861(19981228)402:4<460::AID-CNE3>3.0.CO;2-S

Buijs R.M., 1992, Handbook of Chemical Neuroanatomy, 547

10.1016/0165-3806(94)90015-9

10.1159/000125180

10.1007/BF00238301

10.1016/0165-3806(86)90068-4

10.1126/science.7123245

10.1016/s0891-0618(96)00200-1

10.1016/0304-3940(93)90447-S

10.1016/S0143-4179(97)90054-8

10.1002/cne.901440204

Kalsbeek A., 1992, Handbook of Chemical Neuroanatomy, 63

10.1002/cne.902360409

10.1016/S0166-2236(00)01594-0

10.1016/S0165-0173(97)00006-4

10.1016/0006-8993(88)90055-8

10.1016/0165-3806(91)90063-O

10.1523/JNEUROSCI.18-23-09996.1998

10.1002/cne.902760212

10.1016/0304-3940(89)90654-X

10.1016/s0304-3940(98)00906-9

10.1016/S0165-0173(97)00007-6

10.1002/cne.902370103

Saper C.B., 1987, Handbook of Physiology, the Nervous System, 169

10.1016/0361-9230(86)90018-3

Simmons D.M., 1989, A complete protocol for in situ hybridization of messenger RNAs in brain and other tissues with radio‐labeled single‐stranded RNA probes, J. Histotechnol., 12, 169, 10.1179/014788889794651870

Swanson L.W., 1987, Handbook of Chemical Neuroanatomy, 1

Swanson L.W., 1992, Brain Maps: Structure of the Rat Brain

Vrang N., 1999, Neurochemical characterization of hypothalamic cocaine‐amphetamine‐regulated transcript neurons, J. Neurosci., 19, RC5(1, 10.1523/JNEUROSCI.19-10-j0006.1999

10.1016/s0169-328x(00)00080-2

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European Journal of Neuroscience

Tập 13 Số 9

1733-1744

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