One-Step Purification, Covalent Immobilization, and Additional Stabilization of a Thermophilic Poly-His-Tagged β-Galactosidase from<i>Thermus</i>sp. Strain T2 by using Novel Heterofunctional Chelate−Epoxy Sepabeads

Anspach F. B, 1994, J. Chromatogr., 672, 49

Hemdan E. S., 1989, Proc. Natl. Acad. Sci. U.S.A., 86, 1815, 10.1073/pnas.86.6.1811

Hubert H., 1980, J. Chromatogr., 198, 255, 10.1016/S0021-9673(00)84764-0

Porath J, 1992, Protein Expression Purif., 3, 281, 10.1016/1046-5928(92)90001-D

Porath J., 1975, Nature (London), 258, 599, 10.1038/258598a0

Wang M. Y., 1994, Biotechnol. Bioeng., 43, 356

Woker R., 1992, J. Chromatogr., 584, 92

Beitle R. R., 1997, Immobilization of enzymes and cells. Methods in Biotechnology 1, 343

Brena B., 1994, Biotechnol. Appl. Biochem., 19, 231

Hernaiz M. J., 2000, Enzyme Microb. Technol., 27, 32, 10.1016/S0141-0229(00)00150-2

Hartmeier W, 1985, TIBTECH, 3, 153, 10.1016/0167-7799(85)90104-0

Kennedy J. F., 1990, Chem. Eng. Prog., 45, 89, 10.1016/0009-2509(90)87083-5

Klibanov A. M, 1983, Science, 219, 727, 10.1126/science.219.4585.722

Rosevear A., 1984, J. Chem. Technol. Bioctechnol., 34, 150

Royer G. P, 1980, Catal. Rev., 22, 73

Katchalski-Katzir E., 2000, J. Mol. Catal. B: Enzym., 10, 176, 10.1016/S1381-1177(00)00124-7

Mateo C., 2000, Enzyme Microb. Technol., 26, 515, 10.1016/S0141-0229(99)00188-X

Mateo C., 2002, Prog., 18, 634

Melander W., 1984, J. Chromatogr., 317, 85, 10.1016/S0021-9673(01)91648-6

Smalla K., 1988, Biotechnol. Appl. Biochem., 10, 31, 10.1111/j.1470-8744.1988.tb00003.x

Wheatley J. B., 1993, J. Chromatogr., 644, 16, 10.1016/0021-9673(93)80114-N

Wheatley J. B., 1999, J. Chromatogr., A, 849, 12, 10.1016/S0021-9673(99)00484-7

Mateo C., 2000, Biomacromolecules, 1, 745, 10.1021/bm000071q

Mateo C., 2001, Biotechnol. Bioeng., 76, 277, 10.1002/bit.10019

Benevides, C. CH. P.; Vian, A.; Mateo, C.; Fernández-Lafuente, R.; Guisan, J. M.; García, J. L.; Carrascosa, A. V. 2002. Engineering the β-galactosidase gene fromThermussp. T2 to facilitate its overproduction inEscherichiacoliand its downstream processing by tailor-made metal chelate supports.Appl. Environ. Microbiol., in press.

Hirata H., 1986, J. Bacteriol., 166, 727, 10.1128/jb.166.3.722-727.1986

Hirata H., 1986, J. Bacteriol., 160, 14

Vian A., 1998, Appl. Environ. Microbiol., 6, 2191

Mateo C., 2001, J. Chromatogr., A, 915, 106, 10.1016/S0021-9673(01)00626-4

Novo, 1979, commercial information

Bradford M. M, 1976, Anal. Biochem., 76, 248, 10.1016/0003-2697(76)90527-3

Bastida A., 1998, Biotechnol. Bioeng., 58, 486, 10.1002/(SICI)1097-0290(19980605)58:5<486::AID-BIT4>3.0.CO;2-9

Fernández-Lafuente R., 1999, J. Mol. Catal. B: Enzym., 7, 189

Fernández-Lafuente R., 2001, Biomocromolecules, 2, 104

Laemmli U. K, 1970, Nature, 277, 685

Schuck P, 2000, Biophys. J., 78, 1619, 10.1016/S0006-3495(00)76713-0

Schuck P., 2002, Biophys. J., 82, 1111, 10.1016/S0006-3495(02)75469-6

Koyama Y., 1990, Appl. Envioron. Microbiol., 56, 2254