Oncolytic Properties of a Mumps Virus Vaccine Strain in Human Melanoma Cell Lines
Tóm tắt
The oncolytic potential of the attenuated mumps virus (MV) vaccine strain Leningrad-3 (L-3) was evaluated in a panel of four human metastatic melanoma cell lines. The lines were shown to be susceptible and permissive to MV infection. Efficient MV replication led to death of melanoma cells, but the effect differed among the cell lines. Possible mechanisms mediating the selectivity of MV L-3 towards the cell lines were explored. Replicative and oncolytic activity of MV was found to depend on the expression pattern of type I interferon genes. None of the melanoma cell lines showed induction of expression of the total spectrum of genes required to inhibit virus replication. Based on the results, MV L-3 was assumed to be a promising oncolytic agent for human melanoma cells.
Tài liệu tham khảo
Imyanitov E.N. 2012. Epidemiology and biology of skin tumors. Pract. Oncol. 13, 61–68.
Lech P.J., Russell S.J. 2010. Use of attenuated paramyxoviruses for cancer therapy. Exp. Rev. Vaccines. 9, 1275–1302.
Fuster M.M., Esko J.D. 2005. The sweet and sour of cancer: Glycans as novel therapeutic targets. Nat. Rev. Cancer. 5, 526–542.
Matveeva O.V., Kochneva G.V., Netesov S.V., Onikienko S.B., Chumakov P.M. 2015. Mechanisms of oncolysis by paramyxovirus Sendai. Acta Naturae. 7, 6–16.
Ammour Y.I., Borisova T.K., Zverev V.V. 2013. Measles virus as an oncolytic agent. Vopr. Virusol. S1, 116–131.
Mikhaylova I.N., Morozova L.F., Golubeva V.A., Cheremushkin E.A., Lukashina M.I., Burova O.S., Utyashev I.A., Demidov L.V., Baryshnikov A.Y., Kovalevsky D.A., Beabealashvilli R.Sh., Voronina E.S., Kiselev S.L. 2008. Cancer/testis genes expression in human melanoma cell lines. Melanoma Res. 18, 303–313.
Mikhaĭlova I.N., Lukashina M.I., Baryshnikov A.Iu., Morozova L.F., Burova O.S., Palkina T.N., et al. 2005. Melanoma cell lines as the basis for antitumor vaccine preparation. Vestn. Ross. Akad. Med. Nauk. 7, 37–40.
Ammour Y., Faizuloev E., Borisova T., Nikonova A., Dmitriev G., Lobodanov S., Zverev V. 2013. Quantification of measles, mumps and rubella viruses using real-time quantitative TaqMan-based RT-PCR assay. J. Virol. Methods. 187, 57–64.
Reed L.J., Muench H. 1938. A simple method of estimating fifty percent endpoints. Am. J. Hygiene. 27, 493–497.
Livak K.J., Schmittgen T.D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta DeltaC (T)) method. Methods. 25, 402–408.
Parisien J.P., Bamming D., Komuro A., Ramachandran A., Rodriguez J.J., Barber G., Wojahn R.D., Horvath C.M. 2009. A shared interface mediates paramyxovirus interference with antiviral RNA helicases MDA5 and LGP2. J. Virol. 83, 7252–7260.
Kang D.C., Gopalkrishnan R.V., Wu Q., Jankowsky E., Pyle A.M., Fisher P.B. 2002. mda-5: An interferoninducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties. Proc. Natl. Acad. Sci. U. S. A. 22, 637–642.
Kovacsovics M., Martinon F., Micheau O., Bodmer J.L., Hofmann K., Tschopp J. 2002. Overexpression of Helicard, a CARD-containing helicase cleaved during apoptosis, accelerates DNA degradation. Curr. Biol. 14, 838–843.
Cheon H.J., Borden E.C., Stark G.R. 2014. Interferons and their stimulated genes in the tumor microenvironment. Semin. Oncol. 41, 156–173.
Ivashkiv L.B., Donlin L.T. 2014. Regulation of type I interferon responses. Nat. Rev. Immunol. 14, 36–49.
Khodarev N.N., Beckett M., Labay E., Darga T., Roizman B., Weichselbaum R.R. 2004. STAT1 is overexpressed in tumors selected for radioresistance and confers protection from radiation in transduced sensitive cells. Proc. Natl. Acad. Sci. U. S. A. 101, 1714–1719.
Rickardson L., Fryknäs M., Dhar S., Lövborg H., Gullbo J., Rydåker M., Nygren P., Gustafsson M.G., Larsson R., Isaksson A. 2005. Identification of molecular mechanisms for cellular drug resistance by combining drug activity and gene expression profiles. Br. J. Cancer. 93, 483–492.
Duarte C.W., Willey C.D., Zhi D., Cui X., Harris J.J., Vaughan L.K., Mehta T., McCubrey R.O., Khodarev N.N., Weichselbaum R.R., Gillespie G.Y. 2012. Expression signature of IFN/STAT1 signaling genes predicts poor survival outcome in glioblastoma multiforme in a subtype-specific manner. PLoS One. 7, e29653.