On the Chemistry, Toxicology and Genetics of the Cyanobacterial Toxins, Microcystin, Nodularin, Saxitoxin and Cylindrospermopsin

Sivonen, K, and Jones, G (1999). Toxic Cyanobacteria in Water: A Guide to Their Public Health consequences, Monitoring and Management, E and FN Spon.

Welker, 2006, Cyanobacterial peptides -nature’s own combinatorial biosynthesis, FEMS Microbiol Rev, 30, 530, 10.1111/j.1574-6976.2006.00022.x

Botes, 1985, Structural studies on cyanoginosins-LR, -YR, -YA, and -YM, peptide toxins from Microcystis aeruginosa, J Chem Soc, 1, 2747

Namikoshi, 1998, Seven new microcystins possessing two l-glutamic acid units, isolated from Anabaena sp. strain 186, Chem Res Toxicol, 11, 143, 10.1021/tx970120t

Rinehart, 1994, Structure and biosynthesis of toxins from blue-green algae (cyanobacteria), J App Phycol, 6, 159, 10.1007/BF02186070

Sivonen, 1996, Cyanobacterial toxins and toxin production, Phycologia, 35, 12, 10.2216/i0031-8884-35-6S-12.1

Chorus, I, and Bartram, J (1999). A Guide to their Public Health Consequences, Monitoring and Management, E & FN Spon.

Jochimsen, 1998, Liver failure and death after exposure to microcystins at a hemodialysis center in Brazil, N Engl J Med, 338, 873, 10.1056/NEJM199803263381304

Runnegar, 1991, The uptake of the cyanobacterial hepatotoxin microcystin by isolated rat hepatocytes, Toxicon, 29, 43, 10.1016/0041-0101(91)90038-S

Runnegar, 1995, Microcystin uptake and inhibition of protein phosphatases: effects of chemoprotectants and self-inhibition in relation to known hepatic transporters, Toxicol Appl Pharmacol, 134, 264, 10.1006/taap.1995.1192

Dawson, 1998, The toxicology of microcystins, Toxicon, 36, 953, 10.1016/S0041-0101(97)00102-5

Eriksson, 1990, Hepatocyte deformation induced by cyanobacterial toxins reflects inhibition of protein phosphatases, Biochem Biophys Res Commun, 173, 1347, 10.1016/S0006-291X(05)80936-2

Sahin, 1995, Enzymatic analysis of liver samples from rainbow trout for diagnosis of blue-green algae-induced toxicosis, Am J Vet Res, 56, 1110, 10.2460/ajvr.1995.56.08.1110

Krishnamurthy, 1986, Toxic peptides from freshwater cyanobacteria (blue-green algae). I. Isolation, purification and characterization of peptides from Microcystis aeruginosa and Anabaena flosaquae, Toxicon, 24, 865, 10.1016/0041-0101(86)90087-5

Watanabe, 1988, Toxins contained in Microcystis species of cyanobacteria (blue-green algae), Toxicon, 26, 1017, 10.1016/0041-0101(88)90200-0

Yoshizawa, 1990, Inhibition of protein phosphatases by microcystins and nodularin associated with hepatotoxicity, J Cancer Res Clin Oncol, 116, 609, 10.1007/BF01637082

Nishiwaki, 1991, Rapid purification of protein phosphatase 2A from mouse brain by microcystin-affinity chromatography, FEBS Lett, 279, 115, 10.1016/0014-5793(91)80264-4

Falconer, 1991, Tumor promotion and liver injury caused by oral consumption of cyanobacteria, Environ Toxicol Water Qual, 6, 177, 10.1002/tox.2530060207

Ohta, 1992, Liver tumor promotion by the cyanobacterial cyclic peptide toxin microcystin-LR, J Cancer Res Clin Oncol, 118, 420, 10.1007/BF01629424

Yu, 1995, Primary prevention of hepatocellular carcinoma, J Gastroenterol Hepatol, 10, 674, 10.1111/j.1440-1746.1995.tb01370.x

Yu, S (1989). Drinking Water and Primary Liver Cancer, China academic publishers.

Tillett, 2000, Structural organization of microcystin biosynthesis in Microcystis aeruginosa PCC7806: an integrated peptide-polyketide synthetase system, Chem Biol, 7, 753, 10.1016/S1074-5521(00)00021-1

Christiansen, 2003, Microcystin biosynthesis in planktothrix: genes, evolution, and manipulation, J Bacteriol, 185, 564, 10.1128/JB.185.2.564-572.2003

Rouhiainen, 2004, Genes coding for hepatotoxic heptapeptides (microcystins) in the cyanobacterium Anabaena strain 90, Appl Environ Microbiol, 70, 686, 10.1128/AEM.70.2.686-692.2004

Hicks, 2006, Structural characterisation of in vitro and in vivo intermediates on the loading module of microcystin synthetase, ACS Chem Biol, 1, 93, 10.1021/cb500007v

Nishizawa, 2001, Cyclic heptapeptide microcystin biosynthesis requires the glutamate racemase gene, Microbiology, 147, 1235, 10.1099/00221287-147-5-1235

Sielaff, 2003, The mcyF gene of the microcystin biosynthetic gene cluster from Microcystis aeruginosa encodes an aspartate racemase, Biochem J, 373, 909, 10.1042/bj20030396

Pearson, 2007, Characterization of the 2-hydroxy-acid dehydrogenase McyI, encoded within the microcystin biosynthesis gene cluster of Microcystis aeruginosa PCC7806, J Biol Chem, 282, 4681, 10.1074/jbc.M606986200

Pearson, 2004, Inactivation of an ABC transporter gene, mcyH, results in loss of microcystin production in the cyanobacterium Microcystis aeruginosa PCC 7806, Appl Environ Microbiol, 70, 6370, 10.1128/AEM.70.11.6370-6378.2004

Shi, 1995, Immuno-gold localization of hepatotoxins in cyanobacterial cells, Arch Microbiol, 163, 7, 10.1007/BF00262197

Young, 2005, Immunogold localisation of microcystins in cryosectioned cells of Microcystis, J Struct Biol, 151, 208, 10.1016/j.jsb.2005.05.007

Kaebernick, 2000, Light and the transcriptional response of the microcystin biosynthesis gene cluster, Appl Environ Microbiol, 66, 3387, 10.1128/AEM.66.8.3387-3392.2000

Mikalsen, 2003, Natural variation in the microcystin synthetase operon mcyABC and impact on microcystin production in Microcystis strains, J Bacteriol, 185, 2774, 10.1128/JB.185.9.2774-2785.2003

Mikalsen, 2008, The mosaic structure of the mcyABC operon in Microcystis, Microbiology, 154, 1886, 10.1099/mic.0.2007/015875-0

Sivonen, 1990, Effects of light, temperature, nitrate, orthophosphate, and bacteria on growth of and hepatotoxin production by Oscillatoria agardhii strains, Appl Environ Microbiol, 56, 2658, 10.1128/aem.56.9.2658-2666.1990

Lukac, 1993, Influence of trace metals on growth and toxin production of Microcystis aeruginosa, Toxicon, 31, 293, 10.1016/0041-0101(93)90147-B

Eloff, 1985, Effect of temperature and light on the toxicity and growth of the blue-green alga Microcystis aeruginosa (UV-006), Planta, 163, 55, 10.1007/BF00395897

Song, 1998, Microcystin production of Microcystis viridis (cyanobacteria) under different culture conditions, Phycol Res, 42, 19, 10.1046/j.1440-1835.1998.00120.x

Davis, 2009, The effects of temperature and nutrients on the growth and dynamics of toxic and non-toxic strains of Microcystis during cyanobacteria blooms, Harmful Algae, 8, 715, 10.1016/j.hal.2009.02.004

Tonk, 2005, The microcystin composition of the cyanobacterium Planktothrix agardhii changes toward a more toxic variant with increasing light intensity, Appl Environ Microbiol, 71, 5177, 10.1128/AEM.71.9.5177-5181.2005

Sevilla, 2008, Iron availability affects mcyD expression and microcystin-LR synthesis in Microcystis aeruginosa PCC7806, Environ Microbiol, 10, 2476, 10.1111/j.1462-2920.2008.01663.x

Kaebernick, 2002, Multiple alternate transcripts direct the biosynthesis of microcystin, a cyanobacterial nonribosomal peptide, Appl Environ Microbiol, 68, 449, 10.1128/AEM.68.2.449-455.2002

Sivonen, 1989, Occurrence of the hepatotoxic cyanobacterium Nodularia spumigena in the Baltic Sea and structure of the toxin, Appl Environ Microbiol, 55, 1990, 10.1128/aem.55.8.1990-1995.1989

Jones, 1994, A toxic bloom of Nodularia spumigena mertens in Orielton Lagoon, Tasmania, Aust J Mar Freshwater Res, 45, 787, 10.1071/MF9940787

Heresztyn, 1997, Nodularin concentrations in Lakes Alexandrina and Albert, South Australia, during a bloom of the cyanobacterium (blue-green alga) Nodularia spumigena and degradation of the toxin, Environ Toxicol Water Qual, 12, 273, 10.1002/(SICI)1098-2256(1997)12:4<273::AID-TOX1>3.0.CO;2-5

Nehring, 1993, Mortality of dogs associated with a mass development of Nodularia spumigena (Cyanophyceae) in a brackish lake at the German North Sea coast, J Plankton Res, 15, 867, 10.1093/plankt/15.7.867

Carmichael, 1988, Toxicity and partial structure of a hepatotoxic peptide produced by the cyanobacterium Nodularia spumigena Mertens emend. L575 from New Zealand, Appl Environ Microbiol, 54, 2257, 10.1128/aem.54.9.2257-2263.1988

Galat, 1990, Large-scale patterns of Nodularia spumigena blooms in Pyramid Lake, Nevada, determined from Landsat imagery: 1972–1986, Hydrobiologia, 197, 147, 10.1007/BF00026947

Baker, 1994, Toxicity associated with commonly occurring cyanobacteria in surface waters of the Murray-Darling Basin, Australia, Aust J Mar Freshwater Res, 45, 773, 10.1071/MF9940773

Karjalainen, 2007, Ecosystem consequences of cyanobacteria in the northern Baltic Sea, Ambio, 36, 195, 10.1579/0044-7447(2007)36[195:ECOCIT]2.0.CO;2

Carmichael, WW (1994). The Toxins of Cyanobacteria. Sci Am, 64–72.

Francis, 1878, Poisonous Australian lake, Nature, 18, 11, 10.1038/018011d0

Ohta, 1994, Nodularin, a potent inhibitor of protein phosphatases 1 and 2A, is a new environmental carcinogen in male F344 rat liver, Cancer Res, 54, 6402

Oughtred, 2001, Nodularin uptake by seafood during a cyanobacterial bloom, Environ Toxicol, 16, 468, 10.1002/tox.10004

Falconer, 1992, Toxicity of edible mussels Mytilus edulis growing naturally in an estuary during a water bloom of the blue-green alga Nodularia spumigena, Environ Toxicol Water Qual, 7, 119, 10.1002/tox.2530070203

Tyminska, 2007, Accumulation of nodularin in sediments, mussels, and fish from the Gulf of Gdansk, southern Baltic Sea, Environ Toxicol, 22, 101, 10.1002/tox.20239

Rinehart, 1988, Nodularin, microcystin, and the configuration of Adda, J Am Chem Soc, 110, 8557, 10.1021/ja00233a049

Saito, 2001, Nodularin-Har: a new nodularin from Nodularia, J Nat Prod, 64, 139, 10.1021/np000299z

Beattie, 2000, The cyanobacterium Nodularia PCC7804, of freshwater origin, produces [l-Har2]nodularin, Phytochemistry, 54, 57, 10.1016/S0031-9422(00)00045-5

deSilva, 1992, Motuporin, a potent protein phosphatase inhibitor isolated from the Papua New Guinea sponge Theonella swinhoei Gray, Tetrahedron Lett, 33, 1561, 10.1016/S0040-4039(00)91674-5

Eriksson, 1988, Preliminary characterization of a toxin isolated from the cyanobacterium Nodularia spumigena, Toxicon, 26, 161, 10.1016/0041-0101(88)90168-7

Honkanen, 1991, Cyanobacterial nodularin is a potent inhibitor of type 1 and type 2A protein phosphatases, Mol Pharmacol, 40, 577

An, 1994, Use of a colorimetric protein phosphatase inhibition assay and enzyme linked immunosorbent assay for the study of microcystins and nodularins, Toxicon, 32, 1495, 10.1016/0041-0101(94)90308-5

MacKintosh, 1995, The cyanobacterial toxin microcystin binds covalently to cysteine-273 on protein phosphatase 1, FEBS Lett, 371, 236, 10.1016/0014-5793(95)00888-G

Kelker, 2009, Crystal structures of protein phosphatase-1 bound to nodularin-R and tautomycin: a novel scaffold for structure-based drug design of serine/threonine phosphatase inhibitors, J Mol Biol, 385, 11, 10.1016/j.jmb.2008.10.053

Runnegar, 1995, In vivo and in vitro binding of microcystin to protein phosphatases 1 and 2A, Biochem Biophys Res Commun, 216, 162, 10.1006/bbrc.1995.2605

Bagu, 1997, A molecular basis for different interactions of marine toxins with protein phosphatase-1. Molecular models for bound motuporin, microcystins, okadaic acid, and calyculin A, J Biol Chem, 272, 5087, 10.1074/jbc.272.8.5087

Karjalainen, 2008, Nodularin concentrations in Baltic Sea zooplankton and fish during a cyanobacterial bloom, Mar Biol, 155, 483, 10.1007/s00227-008-1046-4

Persson, 2009, Detection of nodularin in European flounder (Platichthys flesus) in the west coast of Sweden: Evidence of nodularin mediated oxidative stress, Harmful Algae, 8, 832, 10.1016/j.hal.2009.03.003

Vuorinen, 2009, Accumulation and effects of nodularin from a single and repeated oral doses of cyanobacterium Nodularia spumigena on flounder (Platichthys flesus L.), Arch Environ Contam Toxicol, 57, 164, 10.1007/s00244-008-9258-7

Moffitt, 2004, Characterization of the nodularin synthetase gene cluster and proposed theory of the evolution of cyanobacterial hepatotoxins, Appl Environ Microbiol, 70, 6353, 10.1128/AEM.70.11.6353-6362.2004

Kleinkauf, 1996, A nonribosomal system of peptide biosynthesis, Eur J Biochem, 236, 335, 10.1111/j.1432-1033.1996.00335.x

Copp, 2007, Characterization of PPTNs, a cyanobacterial phosphopantetheinyl transferase from Nodularia spumigena NSOR10, J Bacteriol, 189, 3133, 10.1128/JB.01850-06

Jonasson, 2008, Expression of the nodularin synthetase genes in the Baltic Sea bloom-former cyanobacterium Nodularia spumigena strain AV1, FEMS Microbiol Ecol, 65, 31, 10.1111/j.1574-6941.2008.00499.x

Negri, 1995, Sheep mortality associated with paralytic shellfish poisons from the cyanobacterium Anabaena circinalis, Toxicon, 33, 1321, 10.1016/0041-0101(95)00068-W

Reyero, 1999, Evidence of saxitoxin derivatives as causative agents in the 1997 mass mortality of monk seals in the Cape Blanc Peninsula, Nat Toxins, 7, 311, 10.1002/1522-7189(199911/12)7:6<311::AID-NT75>3.0.CO;2-I

Sawyer, 1968, Demonstration of a toxin from Aphanisomenon flosaquae (L.) Ralfs, Can J Microbiol, 14, 1199, 10.1139/m68-201

Shumway, 1995, Phycotoxin-related shellfish poisoning: Bivalve molluscs are not the only vectors, Rev Fish Sci, 3, 1, 10.1080/10641269509388565

Shumway, 1988, The effects of the toxic dinoflagellate, Protogonyaulax tamarensis, on the physiology and behavior of marine molluscs, J Shellfish Res, 7, 132

Hallegraeff, GM, Anderson, DM, and Cembella, AD (1995). Manual on Harmful Marine Microalgae, UNESCO.

Daly, 2004, Marine toxins and nonmarine toxins: convergence or symbiotic organisms?, J Nat Prod, 67, 1211, 10.1021/np040016t

Baker, 2003, GTX(4) imposters: characterization of fluorescent compounds synthesized by Pseudomonas stutzeri SF/PS and Pseudomonas/Alteromonas PTB-1, symbionts of saxitoxin-producing Alexandrium spp, Toxicon, 41, 339, 10.1016/S0041-0101(02)00314-8

Kodoma, 1990, Production of paralytic shellfish toxins by a bacterium Moraxella sp. isolated from Protogonyaulax tamarensis, Toxicon, 28, 707, 10.1016/0041-0101(90)90259-A

Martins, 2003, Reevaluation of production of paralytic shellfish toxin by bacteria associated with dinoflagellates of the Portuguese coast, Appl Environ Microbiol, 69, 5693, 10.1128/AEM.69.9.5693-5698.2003

Castro, 2004, The effect of temperature on growth and production of paralytic shellfish poisoning toxins by the cyanobacterium Cylindrospermopsis raciborskii C10, Toxicon, 44, 483, 10.1016/j.toxicon.2004.06.005

Negri, 2003, Three novel hydroxybenzoate saxitoxin analogues isolated from the dinoflagellate Gymnodinium catenatum, Chem Res Toxicol, 16, 1029, 10.1021/tx034037j

Sako, Y, Kim, CH, and Ishida, Y (1992). Mendelian inheritance of paralytic shellfish poisoning toxin in the marine dinoflagellateAlexandrium. Biosci Biotechnol Biochem, 56692–56694.

Lassus, P, Arzul, G, Erard, P, Gentien, C, and Marcaillou-Le Baut, C (1995). Harmful Marine Algal Blooms, London-Paris-New York.

Velzeboer, 2000, Geographical patterns of occurrence and composition of saxitoxins in the cyanobacterial genus Anabaena (Nostocales, Cyanophyta) in Australia, Phycologia, 39, 395, 10.2216/i0031-8884-39-5-395.1

Mahmood, 1986, Paralytic shellfish poisons produced by the freshwater cyanobacterium Aphanizomenon flosaquae NH-5, Toxicon, 24, 175, 10.1016/0041-0101(86)90120-0

Harada, 1982, Structure of two paralytic shellfish toxins, gonyautoxins V and VI, isolated from a tropical dinoflagellate Pyrodinium bahamense var. compressa, Agric Biol Chem, 46, 1861

Oshima, 1987, Dinoflagellate Gimnodium catenatum as the source of paralytic shellfishtoxins in Tasmanian shellfish, Toxicon, 25, 1105, 10.1016/0041-0101(87)90267-4

Faulkner, D, and Fenical, W (1977). Chemistry and Distribution of Deleterious Dinoflagellate Toxins, Plenum.

Carmichael, 1997, Evidence for paralytic shellfish poisons in the freshwater cyanobacterium Lyngbya wollei (Farlow ex Gomont) comb. nov, Appl Environ Microbiol, 63, 3104, 10.1128/aem.63.8.3104-3110.1997

Humpage, 1994, Paralytic shellfish poisons from australian cyanobacterial blooms, Aust J Mar Freshwater Res, 45, 761, 10.1071/MF9940761

Lagos, 1999, The first evidence of paralytic shellfish toxins in the fresh water cyanobacterium Cylindrospermopsis raciborskii, isolated from Brazil, Toxicon, 37, 1359, 10.1016/S0041-0101(99)00080-X

Ballot, 2010, Paralytic shellfish poisoning toxin-producing cyanobacterium Aphanizomenon gracile in northeast Germany, Appl Environ Microbiol, 76, 1173, 10.1128/AEM.02285-09

Llewellyn, 2006, Saxitoxin, a toxic marine natural product that targets a multitude of receptors, Nat Prod Rep, 23, 200, 10.1039/b501296c

Llewellyn, 2001, Radioreceptor assays for sensitive detection and quantitation of saxitoxin and its analogues from strains of the freshwater cyanobacterium, Anabaena circinalis, Environ Sci Technol, 35, 1445, 10.1021/es001575z

Oshima, 1995, Postcolumn derivatization liquid chromatographic method for paralytic shellfish toxins, J AOAC Int, 78, 528, 10.1093/jaoac/78.2.528

Oshima, 1993, Comparative study on paralytic shellfish toxin profiles of the dinoflagellate Gymnodinium catenatum from three different countries, Mar Biol, 116, 471, 10.1007/BF00350064

Onodera, 1997, New saxitoxin analogues from the freshwater filamentous cyanobacterium Lyngbya wollei, Nat Toxins, 5, 146, 10.1002/19970504NT4

Arakawa, 1995, A new saxitoxin analogue from a xanthid crab Atergatis floridus, Toxicon, 33, 1577, 10.1016/0041-0101(95)00106-9

Arakawa, 1994, Occurrence of carbamoyl-N-hydroxy derivatives of saxitoxin and neosaxitoxin in a xanthid crab Zosimus aeneus, Toxicon, 32, 175, 10.1016/0041-0101(94)90106-6

Zaman, 1998, Occurrence of a methyl derivative of saxitoxin in Bangladeshi freshwater puffers, Toxicon, 36, 627, 10.1016/S0041-0101(97)00086-X

Kim, 2004, The structure of zetekitoxin AB, a saxitoxin analog from the golden frog Atelopus zeteki: a potent sodium channel blocker, Proc Natl Acad Sci USA, 101, 4346, 10.1073/pnas.0400368101

Falconer, IR (1993). Algal Toxins in Seafood and Drinking Water, Academic Press.

Kao, CY, and Levinson, SR (1986). Tetrodotoxin, Saxitoxin, and the Molecular Biology of the Sodium Channel, The New York Academy of Science.

Su, 2004, Saxitoxin blocks l-type ICa, J Pharmacol Exp Ther, 308, 324, 10.1124/jpet.103.056564

Wang, 2003, Saxitoxin is a gating modifier of HERG K+ channels, J Gen Physiol, 121, 583, 10.1085/jgp.200308812

Halstead, BW, and Schantz, EJ (1984). Paralytic shellfish poisoning. WHO Offset Publ, 1–59.

Evans, 1969, Mechanism of saxitoxin and tetrodotoxin poisoning, Br Med Bull, 25, 263, 10.1093/oxfordjournals.bmb.a070715

Satin, 1992, A mutant of TTX-resistant cardiac sodium channels with TTX-sensitive properties, Science, 256, 1202, 10.1126/science.256.5060.1202

Strichartz, 1984, Structural determinabts of the affinity of saxitoxin for neuronal sodium channels, J Gen Physiol, 84, 281, 10.1085/jgp.84.2.281

Tikhonov, 2005, Modeling P-loops domain of sodium channel: homology with potassium channels and interaction with ligands, Biophys J, 88, 184, 10.1529/biophysj.104.048173

Jiang, 2002, The open pore conformation of potassium channels, Nature, 417, 523, 10.1038/417523a

Hall, S, and Reichardt, PB (1990). Marine Toxins Origin, Structure and Pharmacology, American Chemical Society.

Cembella, 1994, Sequestering and putative biotransformation of paralytic shellfish toxins by the sea scallop Placopecten magellanicus -seasonal and spatial scales in natural populations, J Exp Mar Biol Ecol, 180, 1, 10.1016/0022-0981(94)90075-2

Cembella, 1993, Anatomical distribution and spatiotemporal variation in paralytic shellfish toxin composition in two bivalve species from the Gulf of Maine, J Shellfish Res, 12, 389

Kellmann, 2008, Biosynthetic intermediate analysis and functional homology reveal a saxitoxin gene cluster in cyanobacteria, Appl Environ Microbiol, 74, 4044, 10.1128/AEM.00353-08

Neuwald, 1997, GCN5-related histone N-acetyltransferases belong to a diverse superfamily that includes the yeast SPT10 protein, Trends Biochem Sci, 22, 154, 10.1016/S0968-0004(97)01034-7

Hederstedt, 1981, Succinate dehydrogenase--a comparative review, Microbiol Rev, 45, 542, 10.1128/mr.45.4.542-555.1981

Mahmood, 1986, The pharmacology of anatoxin-a(s), a neurotoxin produced by the freshwater cyanobacterium Anabaena flos-aquae NRC 525–17, Toxicon, 24, 425, 10.1016/0041-0101(86)90074-7

Sako, 2001, Purification and characterization of a sulfotransferase specific to N-21 of saxitoxin and gonyautoxin 2+3 from the toxic dinoflagellate Gymnodinium catenatum (Dinophyceae), J Phycol, 37, 1044, 10.1046/j.1529-8817.2001.00119.x

Yoshida, 2002, Purification and characterization of sulfotransferase specific to O-22 of 11-hydroxy saxitoxin from the toxic dinoflagellate Gymnodinium catenatum (dinophyceae), Fish Sci, 68, 634, 10.1046/j.1444-2906.2002.00471.x

Schwedock, 1992, Rhizobium meliloti genes involved in sulfate activation: the two copies of nodPQ and a new locus, sua, Genetics, 132, 899, 10.1093/genetics/132.4.899

Akoh, 2004, GDSL family of serine esterases/lipases, Prog Lipid Res, 43, 534, 10.1016/j.plipres.2004.09.002

Pomati, 2004, Interactions between intracellular Na+ levels and saxitoxin production in Cylindrospermopsis raciborskii T3, Microbiology, 150, 455, 10.1099/mic.0.26350-0

Brown, 1999, The multidrug efflux protein NorM is a prototype of a new family of transporters, Mol Microbiol, 31, 394, 10.1046/j.1365-2958.1999.01162.x

Anderson, 1990, Dynamics and physiology of saxitoxin production by the dinoflagellates Alexandrium spp, Mar Biol, 104, 511, 10.1007/BF01314358

Dias, 2002, Production of paralytic shellfish toxins by Aphanizomenon sp. LMECYA 31 (cyanobacteria), J Phycol, 38, 705, 10.1046/j.1529-8817.2002.01146.x

Gedaria, 2007, Growth response and toxin concentration of cultured Pyrodinium bahamense var. compressum to varying salinity and temperature conditions, Toxicon, 50, 518, 10.1016/j.toxicon.2007.04.021

Steed, 1993, Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon, J Bacteriol, 175, 6797, 10.1128/jb.175.21.6797-6809.1993

Ehria, 2006, NrrA, a nitrogen-responsive response regulator facilitates heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120, Mol Microbiol, 59, 1692, 10.1111/j.1365-2958.2006.05049.x

Forst, 1989, Phosphorylation of OmpR by the Osmosensor EnvZ Modulates Expression of the ompF and ompC Genes in Escherichia coli, Proc Natl Acad Sci USA, 86, 6052, 10.1073/pnas.86.16.6052

Mihali, 2009, Characterisation of the paralytic shellfish toxin biosynthesis gene clusters in Anabaena circinalis AWQC131C and Aphanizomenon sp. NH-5, BMC Biochem, 10, 8, 10.1186/1471-2091-10-8

Kellmann, 2008, Identification of a saxitoxin biosynthesis gene with a history of frequent horizontal gene transfers, J Mol Evol, 67, 526, 10.1007/s00239-008-9169-2

Bourke, 1983, An outbreak of hepato-enteritis (the Palm Island mystery disease) possibly caused by algal intoxication, Toxicon, 21, 45, 10.1016/0041-0101(83)90151-4

Byth, 1980, Palm Island mystery disease, Med J Aust, 2, 40, 10.5694/j.1326-5377.1980.tb131814.x

Saker, 1999, Cattle mortality attributed to the toxic cyanobacterium Cylindrospermopsis raciborskii in an outback region of North Queensland, Environ Toxicol Water Qual, 14, 179, 10.1002/(SICI)1522-7278(199902)14:1<179::AID-TOX23>3.0.CO;2-G

Banker, 1997, Identification of cylindrospermopsin in Aphanizomenon ovalisporum (Cyanophyceae) isolated from Lake Kinneret, Israel, J Phycol, 33, 613, 10.1111/j.0022-3646.1997.00613.x

Harada, 1994, Isolation of cylindrospermopsin from a cyanobacterium Umezakia natans and its screening method, Toxicon, 32, 73, 10.1016/0041-0101(94)90023-X

Li, 2001, First report of the cyanotoxins cylindrospermopsin and deoxycylindrospermopsin from Raphidiopsis curvata (Cyanobacteria), J Phycol, 37, 1121, 10.1046/j.1529-8817.2001.01075.x

Li, 2001, Isolation and identification of the cyanotoxin cylindrospermopsin and deoxy-cylindrospermopsin from a Thailand strain of Cylindrospermopsis raciborskii (Cyanobacteria), Toxicon, 39, 973, 10.1016/S0041-0101(00)00236-1

Preussel, 2006, First report on cylindrospermopsin producing Aphanizomenon flos-aquae (Cyanobacteria) isolated from two German lakes, Toxicon, 47, 156, 10.1016/j.toxicon.2005.10.013

Schembri, 2001, Identification of genes implicated in toxin production in the cyanobacterium Cylindrospermopsis raciborskii, Environ Toxicol, 16, 413, 10.1002/tox.1051

Spoof, 2006, First observation of cylindrospermopsin in Anabaena lapponica isolated from the boreal environment (Finland), Environ Toxicol, 21, 552, 10.1002/tox.20216

Seifert, 2007, First evidence for the production of cylindrospermopsin and deoxy-cylindrospermopsin by the freshwater benthic cyanobacterium, Lyngbya wollei (Farlow ex Gomont) Speziale and Dyck, Harmful Algae, 6, 73, 10.1016/j.hal.2006.07.001

Neilan, 2003, Phylogeography of the invasive cyanobacterium Cylindrospermopsis raciborskii, Mol Ecol, 12, 133, 10.1046/j.1365-294X.2003.01709.x

Ohtani, 1992, Cylindrospermopsin: A potent hepatotoxin from the blue-green alga Cylindrospermopsis raciborskii, J Am Chem Soc, 114, 7941, 10.1021/ja00046a067

Banker, 2001, Uracil Moiety is Required for Toxicity of the Cyanobacterial Hepatotoxin Cylindrospermopsin, J Toxicol Environ Health Part A, 62, 281, 10.1080/009841001459432

Runnegar, 1995, Inhibition of reduced glutathione synthesis by cyanobacterial alkaloid cylindrospermopsin in cultured rat hepatocytes, Biochem Pharmacol, 49, 219, 10.1016/S0006-2952(94)00466-8

Runnegar, 1994, The role of glutathione in the toxicity of a novel cyanobacterial alkaloid cylindrospermopsin in cultured rat hepatocytes, Biochem Biophys Res Commun, 201, 235, 10.1006/bbrc.1994.1694

Runnegar, 2002, In vitro Hepatotoxicity of the Cyanobacterial Alkaloid Cylindrospermopsin and Related Synthetic Analogues, Toxicol Sci, 67, 81, 10.1093/toxsci/67.1.81

Kiss, 2002, Membrane effects of toxins isolated from a cyanobacterium, Cylindrospermopsis raciborskii, on identified molluscan neurones, Comp Biochem Physiol C: Toxicol Pharmacol, 131, 167

Humpage, 2000, Micronucleus induction and chromosome loss in transformed human white cells indicate clastogenic and aneugenic action of the cyanobacterial toxin, cylindrospermopsin, Mutat Res, 472, 155, 10.1016/S1383-5718(00)00144-3

Froscio, 2003, Cylindrospermopsin-induced protein synthesis inhibition and its dissociation from acute toxicity in mouse hepatocytes, Environ Toxicol Water Qual, 18, 243, 10.1002/tox.10121

Norris, 1999, Deoxycylindrospermopsin, an analog of cylindrospermopsin from Cylindrospermopsis raciborskii, Environ Toxicol Water Qual, 14, 163, 10.1002/(SICI)1522-7278(199902)14:1<163::AID-TOX21>3.0.CO;2-V

Terao, 1994, Electron microscopic studies on experimental poisoning in mice induced by cylindrospermopsin isolated from blue-green alga Umezakia natans, Toxicon, 32, 833, 10.1016/0041-0101(94)90008-6

Wiegand, 2005, Ecotoxicological effects of selected cyanobacterial secondary metabolites: a short review, Toxicol Appl Pharmacol, 203, 201, 10.1016/j.taap.2004.11.002

Mihali, 2008, Characterization of the gene cluster responsible for cylindrospermopsin biosynthesis, Appl Environ Microbiol, 74, 716, 10.1128/AEM.01988-07

Burgoyne, 2000, Biosynthesis of cylindrospermopsin, J Org Chem, 65, 152, 10.1021/jo991257m

Kellmann, 2006, Functional modeling and phylogenetic distribution of putative cylindrospermopsin biosynthesis enzymes, J Mol Evol, 62, 267, 10.1007/s00239-005-0030-6

Sukenik, 2002, A novel gene encoding amidinotransferase in the cylindrospermopsin producing cyanobacterium Aphanizomenon ovalisporum, FEMS Microbiol Lett, 209, 87, 10.1111/j.1574-6968.2002.tb11114.x

Baldwin, 1977, Rules for ring closure: ring formation by conjugate addition of oxygen nucleophiles, J Org Chem, 42, 3846, 10.1021/jo00444a011

Norris, 2002, Hepatic xenobiotic metabolism of cylindrospermopsin in vivo in the mouse, Toxicon, 40, 471, 10.1016/S0041-0101(01)00243-4

Saker, 1999, Two morphological forms of cylindrospermopsis raciborskii (cyanobacteria) isolated from Solomon dam, palm island, Queensland, J Phycol, 35, 599, 10.1046/j.1529-8817.1999.3530599.x

Hansel, 2001, Cloning and characterisation of a hyp gene cluster in the filamentous cyanobacterium Nostoc sp. strain PCC 73102, FEMS Microbiol Lett, 201, 59, 10.1111/j.1574-6968.2001.tb10733.x

Tamagnini, 2002, Hydrogenases and Hydrogen Metabolism of Cyanobacteria, Microbiol Mol Biol Rev, 66, 1, 10.1128/MMBR.66.1.1-20.2002