On-line electrochemical measurements of cerebral hypoxanthine of freely moving rats

Science in China Series B: Chemistry - Tập 52 - Trang 1677-1682 - 2009
ZiPin Zhang1,2, YuQing Lin1, LanQun Mao1
1Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China
2Graduate Uniresity, Chinese Academy of Science, Beijing, China

Tóm tắt

This study demonstrates an on-line method for continuous measurements of cerebral hypoxanthine in the freely moving rats with integration of selective electrochemical biosensing with in vivo microdialysis sampling. The selective electrochemical biosensing is achieved by using xanthine oxidase (XOD) as the specific sensing element and Prussian blue (PB) as the electrocatalyst for the reduction of H2O2 generated from the oxidase-catalyzed reaction. The method is virtually interference-free from the coexisting electroactive species in the brain and exhibits a good stability and reproducibility. Upon integrated with in vivo microdialysis, the on-line method is well suitable for continuous measurements of cerebral hypoxanthine of freely moving rats, which is illustrated by the measurements of the microdialysates after the hypoxanthine standard was externally infused into the rat brain. This study essentially offers a facile on-line electrochemical approach to continuous measurements of cerebral hypoxanthine and could find some interesting applications in physiological and pathological investigations associated with hypoxanthine.

Tài liệu tham khảo

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