On-filter direct amplification of Legionella pneumophila for rapid assessment of its abundance and viability

Ahmad, 2011, A CCD-based fluorescence imaging system for real-time loop-mediated isothermal amplification-based rapid and sensitive detection of waterborne pathogens on microchips, Biomed. Microdevices, 13, 929, 10.1007/s10544-011-9562-2 Anderson, 2016 Associated Press, 2016 Ballard, 2000, Detection of Legionella pneumophila using a real-time PCR hybridization assay, J. Clin. Microbiol., 38, 4215, 10.1128/JCM.38.11.4215-4218.2000 Behets, 2007, Development and evaluation of a Taqman duplex real-time PCR quantification method for reliable enumeration of Legionella pneumophila in water samples, J. Microbiol. Methods, 68, 137, 10.1016/j.mimet.2006.07.002 Bonetta, 2010, Evaluation of Legionella pneumophila contamination in Italian hotel water systems by quantitative real-time PCR and culture methods, J. Appl. Microbiol., 108, 1576, 10.1111/j.1365-2672.2009.04553.x Cameron, 1984, The stabilization of “dissolved” iron in freshwaters, Water Res., 18, 179, 10.1016/0043-1354(84)90067-8 Chang, 2010, Comparison of ethidium monoazide and propidium monoazide for the selective detection of viable Legionella cells, Jpn. J. Infect. Dis., 63, 119, 10.7883/yoken.63.119 Chien, 2004, The genomic sequence of the accidental pathogen Legionella pneumophila, Science, 305, 1966, 10.1126/science.1099776 Delgado-Viscogliosi, 2009, Viability PCR, a culture-independent method for rapid and selective quantification of viable Legionella pneumophila cells in environmental water samples, Appl. Environ. Microbiol., 75, 3502, 10.1128/AEM.02878-08 Ditommaso, 2014, Overestimation of the Legionella spp. load in environmental samples by quantitative real-time PCR: pretreatment with propidium monoazide as a tool for the assessment of an association between Legionella concentration and sanitary risk, Diagn Microbiol. Infect. Dis., 80, 260, 10.1016/j.diagmicrobio.2014.09.010 Dooling, 2015, Active bacterial core surveillance for Legionellosis - United States, 2011-2013, Morb. Mortal. Wkly. Rep., 64, 1190, 10.15585/mmwr.mm6442a2 Feeley, 1979, Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila, J. Clin. Microbiol., 10, 437, 10.1128/JCM.10.4.437-441.1979 Füchslin, 2010, Rapid and quantitative detection of Legionella pneumophila applying immunomagnetic separation and flow cytometry, Cytom. Part A J. Int. Soc. Anal. Cytol., 77, 264 Herbers, 2013 ISO, 1998 Joly, 2006, Quantitative real-time Legionella PCR for environmental water samples: data interpretation, Appl. Environ. Microbiol., 72, 2801, 10.1128/AEM.72.4.2801-2808.2006 Krøjgaard, 2011, Detection of Legionella by quantitative-polymerase chain reaction (qPCR) for monitoring and risk assessment, BMC Microbiol., 11, 254, 10.1186/1471-2180-11-254 Lee, 2011, An international trial of quantitative PCR for monitoring Legionella in artificial water systems, J. Appl. Microbiol., 110, 1032, 10.1111/j.1365-2672.2011.04957.x Lu, 2011, LAMP-based method for a rapid identification of Legionella spp. and Legionella pneumophila, Appl. Microbiol. Biotechnol., 92, 179, 10.1007/s00253-011-3496-8 Lucas, 2016, Legionnaires' outbreaks preventable with water management programs, ASHRAE J. Novemb., 84 Mentasti, 2015, Design and validation of a qPCR assay for accurate detection and initial serogrouping of Legionella pneumophila in clinical specimens by the ESCMID Study Group for Legionella Infections (ESGLI), Eur. J. Clin. Microbiol. Infect. Dis., 34, 1387, 10.1007/s10096-015-2363-4 Morio, 2008, Real-time PCR assay for the detection and quantification of Legionella pneumophila in environmental water samples: utility for daily practice, Int. J. Hyg. Environ. Health, 211, 403, 10.1016/j.ijheh.2007.06.002 Nocker, 2009, Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques, FEMS Microbiol. Lett., 291, 137, 10.1111/j.1574-6968.2008.01429.x Nocker, 2007, Use of propidium monoazide for live/dead distinction in microbial ecology, Appl. Environ. Microbiol., 73, 5111, 10.1128/AEM.02987-06 Notomi, 2000, Loop-mediated isothermal amplification of DNA, Nucleic Acids Res., 28, E63, 10.1093/nar/28.12.e63 NYSDOH, 2015 NYSDOH, 2016 Parr, 2015, Legionellosis on the rise: a review of guidelines for prevention in the United States, J. Public Health Manag. Pract., 21, E17, 10.1097/PHH.0000000000000123 Pei, 2010, Diversity of 16S rRNA genes within individual prokaryotic genomes, Appl. Environ. Microbiol., 76, 3886, 10.1128/AEM.02953-09 Rankin, 2002, Macrophage-induced genes of Legionella pneumophila: protection from reactive intermediates and solute imbalance during intracellular growth. Infection and immunity, Infect. Immun., 70, 3637, 10.1128/IAI.70.7.3637-3648.2002 Rasband, 1997 Schwake, 2016, Legionella DNA markers in tap water coincident with spike in Legionnaires' disease in Flint, MI, Environ. Sci. Technol. Lett., 3, 311, 10.1021/acs.estlett.6b00192 Slimani, 2012, Evaluation of propidium monoazide (PMA) treatment directly on membrane filter for the enumeration of viable but non cultivable Legionella by qPCR, J. Microbiol. Methods, 88, 319, 10.1016/j.mimet.2011.12.010 Stedtfeld, 2016, Direct loop mediated isothermal amplification on filters for quantification of Dehalobacter in groundwater, J. Microbiol. Methods, 131, 61, 10.1016/j.mimet.2016.09.025 Stedtfeld, 2015, Static self-directed sample dispensing into a series of reaction wells on a microfluidic card for parallel genetic detection of microbial pathogens, Biomed. Microdevices, 17, 89, 10.1007/s10544-015-9994-1 Stedtfeld, 2012, Gene-Z: a device for point of care genetic testing using a smartphone, Lab Chip, 12, 1454, 10.1039/c2lc21226a Toppo, 2015 USEPA, 2001 USEPA, 2016 Vanysacker, 2014, Development and evaluation of a TaqMan duplex real-time PCR quantification method for reliable enumeration of Candidatus microthrix, J. Microbiol. Methods, 97, 6, 10.1016/j.mimet.2013.11.016 Wellinghausen, 2001, Detection of Legionella in hospital water samples by quantitative real-time LightCycler PCR, Appl. Environ. Microbiol., 67, 3985, 10.1128/AEM.67.9.3985-3993.2001 WHO, 2007 Williams, 2017, Implications of direct amplification for measuring antimicrobial resistance using point-of-care devices, Anal. Methods, 9, 1229, 10.1039/C6AY03405E Yáñez, 2011, Quantification of viable Legionella pneumophila cells using propidium monoazide combined with quantitative PCR, J. Microbiol. Methods, 85, 124, 10.1016/j.mimet.2011.02.004 Zhao, 2013, Rapid detection of viable Escherichia coli O157 by coupling propidium monoazide with loop-mediated isothermal amplification, J. Microbiol. Biotechnol., 23, 1708, 10.4014/jmb.1306.06003