Olsenella scatoligenes sp. nov., a 3-methylindole- (skatole) and 4-methylphenol- (p-cresol) producing bacterium isolated from pig faeces

International Journal of Systematic and Evolutionary Microbiology - Tập 65 Số Pt_4 - Trang 1227-1233 - 2015
Xiaoqiong Li1, Rikke Lassen Jensen2, Ole Højberg1, Nuria Canibe1, Bent Borg Jensen1
1Department of Animal Science, Aarhus University, Tjele, Denmark
2Department of Large Animal Science, University of Copenhagen, Copenhagen, Denmark

Tóm tắt

Strain SK9K4T, which is a strictly anaerobic, non-motile, non-sporulating, Gram-stain-positive, saccharolytic coccobacillus, was isolated from pig faeces. SK9K4T metabolized indol-3-acetic acid to 3-methylindole (skatole), which is the main contributor to boar taint; it also produced 4-methylphenol (p-cresol) from p-hydroxyphenylacetic acid. Phylogenetic analyses, based on 16S rRNA gene sequences, revealed that the isolate represented a new lineage within the genus Olsenella of the family Atopobiaceae . Strain SK9K4T was most closely related to the type strains of the three species of the genus Olsenella with validly published names; Olsenella profusa DSM 13989T (93.6 %), Olsenella uli DSM 7084T (93.5 %) and Olsenella umbonata DSM 22620T (92.7 %). DNA–DNA relatedness values of strain SK9K4T with O. profusa , O. uli and O. umbonata were 28.3 %, 69.1 % and 27.2 %, respectively. The genomic DNA G+C content was 62.1 mol% and the major cellular fatty acids (constituting >10 % of the total) were C14 : 0 and C18 : 1ω9c. The major end product of glucose fermentation was lactic acid, with minor amounts of acetic acid and formic acid; no H2 was produced. Discrepancies in the fatty acid profiles, the MALDI-TOF mass spectra of cell extracts and the physiological and biochemical characteristics differentiated strain SK9K4T from other species of the genus Olsenella and indicate that the isolate represents a novel species within this genus. The name Olsenella scatoligenes sp. nov., is proposed and the type strain is SK9K4T ( = JCM 19907T = DSM 28304T).

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Thông tin xuất bản

International Journal of Systematic and Evolutionary Microbiology

Tập 65 Số Pt_4

1227-1233

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