Olfactory relationships of the telencephalon and diencephalon in the rabbit. III. The ipsilateral centrifugal fibers to the olfactory bulbar and retrobulbar formations
Tóm tắt
The axoplasmic retrograde transport of horseradish peroxidase (HRP) from axon terminals to their parent cell bodies and histochemical fluorescence microscopy have been used to study the ipsilateral centrifugal fibers to the olfactory bulbs and anterior olfactory nucleus in the rabbit. Focal injections of peroxidase were placed unilaterally into the main or accessory olfactory bulb or into the anterior olfactory nucleus. In animals with injected HRP confined within the main bulb, perikarya retrogradely labeled with the protein in the ipsilateral forebrain were observed in the anterior prepyriform cortex, horizontal limb of the nucleus of the diagonal band, and far lateral preoptic and rostral lateral hypothalamic areas. Brain stem cell groups that contained HRP‐positive somata include the locus coeruleus and midbrain dorsal raphe nucleus. Except for the prepyriform cortex, the basal forebrain structures with labeled perikarya correlate well with locations of cell bodies containing acetylcholinesterase and choline acetyltransferase. These somata may represent a cholinergic afferent system to the main olfactory bulb. Peroxidase‐labeled cell bodies in the locus coeruleus and midbrain raphe are indicative of noradrenergic and serotonergic innervations respectively of the olfactory bulb. In rabbits in which peroxidase was injected or diffused into the accessory olfactory bulb and anterior olfactory nucleus, HRP‐positive somata were identified in the prepyriform cortex bilaterally, the horizontal limb of the diagonal band nucleus, lateral hypothalamic region, nucleus of the lateral olfactory tract, corticomedial complex of the amygdala, mitral and tufted cell layers of the ipsilateral main olfactory bulb, locus coeruleus, and the midbrain raphe. Evidence for centrifugal fibers to the accessory olfactory bulb from the corticomedial complex of the amygdala, locus coeruleus, and possibly the nucleus of the lateral olfactory tract and midbrain raphe is discussed. A similar distribution of labeled perikarya in the forebrain and brain stem was seen in rats in which peroxidase injected into the main olfactory bulb had spread into the accessory bulb and anterior olfactory nucleus.
Histochemical fluorescence microscopy of the main and accessory olfactory bulbs in the rabbit and rat revealed fine caliber, green fluorescent fibers and varicosities predominantly in the granule cell layer and less so among cells in the glomerular layer. In sections through the root of the main olfactory bulb, a similar fluorescence was seen in the deep half of the plexiform layer of the pars externa of the anterior olfactory nucleus. These fluorescent fibers likely represent the noradrenergic innervation of the olfactory bulbar and retrobulbar formations. A fluorescent yellow hue was observed in the glomerular layer of the main bulb and may signify a serotonergic innervation of this lamina.
The results of this study provide continued support for the existence of main and accessory olfactory systems and offer greater insight into the organization of the olfactory brain and the interrelationship of the hypothalamus and olfaction.
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Tài liệu tham khảo
Bloom F. E., 1964, Analysis of individual rabbit olfactory bulb neuron responses to the microelectrophoresis of accetylcholine, norepinepherine, and serotonin synergists and antagonists, J. Pharmacol. Exptl. Therap., 146, 16
Broadwell R. D., 1974, Olfactory relationships of the telencephalon in the rabbit as studied by the autoradiographic anterograde and horseradish peroxidase retrograde intraaxonal protein transport tracing techniques, Anat. Rec., 178, 316
Broadwell R. D., 1975, Olfactory relationships of the telencephalon and diencephalon in the rabbit. 1. An autoradiographic study of the efferent connections of the main and accessory olfactory bulbs, J. Comp. Neur., 16, 329, 10.1002/cne.901630306
Cajal S. R.1890Origen y terminación de las fibras nervioosas olfactorias.Gaz. sanit. de Barcelona.
Cajal S. R., 1901, La corteza olfactivo del hombre y mamiferos, Trab. d. lab. de invest. biol. Univ. de Madrid, 1, 1
Cajal S. R., 1955, Studies on the Cerebral Cortex
Carlsson A., 1962, Cellular localization of brain monoamines, Acta. Physiol. Sand. (Suppl.), 196, 1
Daval G., 1974, Electrophysiological studies of centrifugal and centripetal connections of the anterior olfactory nucleus, Brain Res., 78, 345, 10.1016/0006-8993(74)90923-8
Dennis B. J., 1975, Olfactory bulb connections with basal rhinencephalon in the ferret: an evoked potential and neuroanatomical study, J. Comp. Neur., 154, 129, 10.1002/cne.901590108
Falck B., 1962, Observations on the possibilities for the cellular localization of monoamines with a fiuorescence method, Acta Physiol. Scand., 56, 1
Golgi C.1875Sulla fina struttura dei bulbi olfactorii.Reggio‐Emilia.
Haberly L. B., 1975, Afferent connections of the olfactory cortex, Soc. Neurosci., 1, 14
Halperin M., 1973, Olfactory bulb and accessory bulb projections in the snake, Thranophis sirtalis, Anat. Rec., 175, 337
Heimer L., 1968, Synaptic distribution of centripetal and centrifugal nerve fibers in the olfactory system of the rat. An experimental anatomical study, J. Anat. (London), 103, 413
Heimer L., 1975, Golgi Centennial Symposium Proc., 177
Jacobowitz D. M., 1975, Anatomical Neuroendocrinology, 368
Kerr D. I. B., 1955, An investigation of olfactory centrifugal system, J. Neurophysiol., 18, 362, 10.1152/jn.1955.18.4.362
Krettek J. E. andJ. L.Price1974Connection of the amygdala with the bed nucleus of the stria terminalis and the hypothalamus in the rat and cat. Soc. Neurosci. 4th Annual Meeting. Abstract book p.243.
Lohman A. H. M., 1963, The anterior olfactory lobe of the guinea pig. A descriptive and experimental anatomical study, Acta Anat., 53, 1
Lundberg P. O., 1960, Cortico‐hypothalamic connections in the rabbit, Acta Physiol. Scand., 49, 1
Mancia M., 1962, Reticular control of single neurons in the olfactory bulb, Arch. Ital. Biol., 100, 463
McLennan H., 1971, The pharmacology of inhibition of mitral cells in the olfactory bulb, Brain Res., 24, 177, 10.1016/0006-8993(71)90026-6
Miliaressis E., 1975, Catecholamine concentration of discrete brain areas following self‐stimulation in the ventromedial tegmentum of the rat, Brain Res., 100, 142, 10.1016/0006-8993(75)90259-0
Pinching A. J., 1972, The termination of centrifugal fibers in the glomerular layer of the olfactory bulb, J. Cell Sci., 10, 621, 10.1242/jcs.10.3.621
Powell T. P. S., 1965, The central olfactory connections, J. Anat., 99, 791
Price J. L., 1970, An electron microscopic study of the termination of the afferent fibres to the olfactory bulb from the cerebral hemisphere, J. Cell. Sci., 7, 157, 10.1242/jcs.7.1.157
Price J. L., 1970, An experimental study of the origin and the course of the centrifugal fibres to the olfactory bulb in the rat, J. Anat. (London), 107, 215
Price J. L., 1970, The afferent connections of the nucleus of the horizontal limb of the diagonal band, J. Anat. (London), 107, 239
Reese T. S., 1970, Ciba Found. Symp. Taste and Smell in Vertebrates, 115
Reese T. S., 1972, Structure and function of synapses, 121
Sherlock D. A., 1974, Retrograde transport of horseradish peroxidase in the magnocellular neurosecretory system of the rat, Brain Res., 88, 403, 10.1016/0006-8993(75)90653-8
Valverde H. P. M., 1969, The anterior commissure of the rabbit, Acta Anatomica, 57, 1
Van Gehuchten A., 1891, Le bulbe olfactif chez quelques mammifères, La Cellule, 7, 205
Winkler C., 1918, On the olfactory tract in the rabbit, Opera omnia Haarlem: F. Bohm, 5, 397