O‐linked N‐acetylglucosamine levels in cerebellar neurons respond reciprocally to pertubations of phosphorylation

FEBS Journal - Tập 262 Số 3 - Trang 824-831 - 1999
Lee S. Griffith1, Brigitte Schmitz1
1Department of Biochemistry, Institute for Animal Anatomy and Physiology, University of Bonn, Germany.

Tóm tắt

The novel intracellular carbohydrate O‐linked N‐acetylglucosamine (O‐GlcNAc) is present on proteins ranging from those of viruses to those of humans and include cytosolic, nuclear and plasma‐membrane proteins. In this report we have examined the effect of manipulation of phosphorylation on the levels of O‐GlcNAc in cerebellar neurons from early postnatal mice. Our results indicate a reciprocal response of O‐GlcNAc levels to phosphorylation. Activation of protein kinase A or C, for example, results in reduced levels of O‐GlcNAc specifically in the fraction of cytoskeletal and cytoskeleton‐associated proteins, while inhibition of the same kinases results in increased levels of O‐GlcNAc. These data are in keeping with a reciprocal action of O‐GlcNAc with respect to phosphorylation and suggest that this modification may have a role in signal transduction.

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