Nutrition and bioprocess development for efficient biosynthesis of an antitumor compound from marine-derived fungus
Tóm tắt
An integrated nutrition and bioprocess strategy was developed for improving the biosynthesis of an antitumor compound, 1403C, by a marine-derived fungus, Halorosellinia sp. (no. 1403). First, statistical design strategies were synthetically applied to optimize the nutritional composition. The resulting 1403C production reached 2.07 g/l, which was 143.5 % higher than the original production. However, it only produced 0.44 g/l of 1403C in 5-l bioreactor fermentation. Thus, the operating parameters including culture pH, dissolved oxygen, agitation speed, impeller type and inoculum level were considered to improve the fermentation process, and an effective control strategy for 1403C production by Halorosellinia sp. submerged in a 5-l bioreactor was established. When inoculating 0.22 g/l dry biomass, controlling dissolved oxygen not lower than 30 % during the growth phase but ranging between 30 and 40 % during the stationary phase, using a double-layer six-flat-blade Rushton disc turbine agitated at 400 rpm, keeping short-term low pH and rapid-rising pH with glucose starvation, the highest 1403C production was finally obtained at 1.32 g/l, which was promoted by 200 % compared to before optimization. Fermentation scale-up was finally performed in a 500-l bioreactor, and 1403C production of 1.09 g/l was obtained.
Tài liệu tham khảo
Albaek MO, Gernaey KV, Hansen MS, Stocks SM (2011) Modeling enzyme production with Aspergillus oryzae in pilot scale vessels with different agitation, aeration, and agitator types. Biotechnol Bioeng 108:1828–1840
Alonso S, Rendueles M, Dίaz M (2012) Role of dissolved oxygen availability on lactobionic acid production from whey by Pseudomonas taetrolens. Bioresour Technol 109:140–147
Bandaiphet C, Prasertsan B (2006) Effect of aeration and agitation rates and scale-up on oxygen transfer coefficient, k La in exopolysaccharide production from Enterobacter cloacae WD7. Carbohydr Polym 66:216–228
Bhadury P, Mohammad BT, Wright PC (2006) The current status of natural products from marine fungi and their potential as anti-infective agents. J Ind Microbiol Biotechnol 33:325–337
Cai M, Zhou X, Lu J, Fan W, Niu C, Zhou J, Sun X, Kang L, Zhang Y (2011) Enhancing aspergiolide A production from a shear-sensitive and easy-foaming marine-derived filamentous fungus Aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor. Bioresour Technol 102:3584–3586
Cai M, Zhou X, Sun X, Tao K, Zhang Y (2009) Statistical optimization of medium composition for aspergiolide A production by marine-derived fungus Aspergillus glaucus. J Ind Microbiol Biotechnol 36(3):381–389
Cheng Z, Tang W, Su Z, Cai Y, Sun S, Chen Q, Wang F, Lin Y, She Z, Vrijmoed LLP (2008) Identification of mangrove endophytic fungus 1403 (Fusarium proliferatum) based on morphological and molecular evidence. J Forestry Res 19:219–224
Damare S, Singh P, Raghukumar S (2012) Biotechnology of marine fungi. Prog Mol Subcell Biol 53:277–297
Desantis C, Siegel R, Bandi P, Jemal A (2011) Breast cancer statistics. CA Cancer J Clin 61:409–418
Garcia-Ochoa F, Gomez E (2009) Bioreactor scale-up and oxygen transfer rate in microbial processes: an overview. Biotechnol Adv 27:153–176
Garcia-Ochoa F, Gomez E, Santos VE, Merchuk JC (2010) Oxygen uptake rate in microbial processes: an overview. Biochem Eng J 49:289–307
Gao X, Qiao SY, Lu W (2009) Determination of an economical medium for growth of Lactobacillus fermentum using response surface methodology. Lett Appl Microbiol 49:556–561
Gerengi H, Darowicki K, Slepski P, Bereket G, Ryl J (2009) Investigation effect of benzotriazole on the corrosion of brass-MM55 alloy in artificial seawater by dynamic EIS. J Solid State Electrochem 14:897–902
Gibbs PA, Seviour RJ, Schmid F (2000) Growth of filamentous fungi in submerged culture: problems and possible solutions. Crit Rev Biotechnol 20:17–48
Hovanec TA, Coshland JL (2004) A chemical analysis of select trace elements in synthetic sea salts and natural seawater. Sea Scope 21:1–4
Junker BH, Stanik M, Barna C, Salmon P, Paul E, Buckland BC (1998) Influence of impeller type on power input in fermentation vessels. Bioprocess Eng 18:401–412
Kang L, Cai M, Fan W, Lu J, Zhou X, Zhang Y (2011) Optimization of fermentation conditions for production of novel anticancer compound 1403C in submerged culture of marine mangrove endophytic fungus Halorosellinia sp. (In chinese). Biotechnol Bull 12:199–204
Kim HJ, Kim JH, Oh HJ, Shin CS (2002) Morphology control of Monascus cells and scale-up of pigment fermentation. Process Biochem 38:649–655
Kim HM, Park MK, Yun JW (2006) Culture pH affects exopolysaccharide production in submerged mycelial culture of Ganoderma lucidum. Appl Biochem Biotechnol 134:249–262
Klöckner W, Büchs J (2012) Advances in shaking technologies. Trends Biotechnol 30:307–314
Lu S, Wang J, Niu Y, Yang J, Zhou J, Yuan Y (2012) Metabolic profiling reveals growth related FAME productivity and quality of Chlorella sorokiniana with different inoculum sizes. Biotechnol Bioeng 109:1651–1662
Mu W, Liu F, Jia J, Chen C, Zhang T, Jiang B (2009) 3-Phenyllactic acid production by substrate feeding and pH-control in fed-batch fermentation of Lactobacillus sp. SK007. Bioresour Technol 100:5226–5229
Pelizer LH, Danesi EDG, Rangel CO, Sassano CEN, Carvalho JCM, Sato S, Moraes IO (2003) Influence of inoculum age and concentration in Spirulina platensis cultivation. J Food Eng 56:371–375
Rateb ME, Ebel R (2011) Secondary metabolites of fungi from marine habitats. Nat Prod Rep 28:290–344
Tang Y, Zhang W, Zhong J (2009) Performance analyses of a pH-shift and DOT-shift integrated fed-batch fermentation process for the production of ganoderic acid and Ganoderma polysaccharides by medicinal mushroom Ganoderma lucidum. Bioresour Technol 100:1852–1859
Wang LP, Ridgway D, Gu TY, Moo-Young M (2005) Bioprocessing strategies to improve heterologous protein production in filamentous fungal fermentation. Biotechnol Adv 23:115–129
Weinberg ED (1990) Roles of trace metals in transcriptional control of microbial secondary metabolism. Biol Metals 2:191–196
Xia J, Wang Y, Zhang S, Chen N, Yin P, Zhuang Y, Chu J (2009) Fluid dynamics investigation of variant impeller combinations by simulation and fermentation experiment. Biochem Eng J 43:252–260
Xie G, Zhu X, Li Q, Gu M, He Z, Wu J, Li J, Lin Y, Li M, She Z, Yuan J (2010) SZ-685C, a marine anthraquinone, is a potent inducer of apoptosis with anticancer activity by suppression of the Akt/FOXO pathway. Br J Pharmacol 159:689–697
Yu C, Cai M, Kang L, Zhang Y, Zhou X (2012) Significance of seed culture methods on mycelial morphology and production of a novel anti-cancer anthraquinone by marine mangrove endophytic fungus Halorosellinia sp. (No. 1403). Process Biochem 47:422–427
Yin Y, Gao Q, Zhang F, Li Z (2012) Medium optimization for the high yield production of single (+)-terrein by Aspergillus terreus strain PF26 derived from marine sponge Phakellia fusca. Process Biochem 47:887–891
Zhang J, Tao L, Liang Y, Chen L, Mi Y, Zheng L, Wang F, She Z, Lin Y, To KKW, Fu L (2010) Anthracenedione derivatives as anticancer agents isolated from secondary metabolites of the mangrove endophytic fungi. Mar Drugs 8:1469–1481
Zhu H, Cao C, Zhang S, Zhang Y, Zou W (2011) pH-control modes in a 5-L stirred-tank bioreactor for cell biomass and exopolysaccharide production by Tremella fuciformis spore. Bioresour Technol 102:9175–9178