Nucleic acid crystallization and X-ray crystallography facilitated by single selenium atom

Springer Science and Business Media LLC - Tập 10 - Trang 196-202 - 2016
Wen Zhang1,2,3, Jack W. Szostak2, Zhen Huang1,3
1Department of Chemistry, Georgia State University, Atlanta, USA
2Howard Hughes Medical Institute, Department of Molecular Biology, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, USA
3College of Life Sciences, Sichuan University, Chengdu, China

Tóm tắt

X-ray crystallography is a powerful strategy for 3-D structure determination of macromolecules, such as nucleic acids and protein-nucleic acid complexes. However, the crystallization and phase determination are the major bottle-neck problems in crystallography. Recently we have successfully developed synthesis and strategy of selenium-derivatized nucleic acids (SeNA) for nucleic acid crystallography. SeNA might not only provide the rational strategies to solve the phase determination problem, but also offer a potential strategy to explore crystallization solutions.

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