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Protein hạt nhân 780BP từ bông cải chứa liên kết một yếu tố trong trình khởi động gene 780 của T-DNA
Tóm tắt
Một vị trí 16 bp của liên kết protein đã được xác định trong trình khởi động của gene 780 của T-DNA. Các tương tác DNA-protein cụ thể đã được chứng minh giữa một oligonucleotide hai sợi chứa yếu tố này (5′-TTGAAAAATCAACGCT-3′) và một protein được chiết xuất từ các mẫu nhân của hoa bông cải. Các bazơ cụ thể cần thiết cho hoạt động liên kết này (protein liên kết 780; 780BP) đã được xác định thông qua các nghiên cứu cạnh tranh động học với các oligonucleotide đột biến, các thí nghiệm can thiệp methyl hóa và hình ảnh hóa dấu chân DNAse I. Việc liên kết 780BP không bị cạnh tranh ngay cả khi có tới 1000 lần dư thừa các yếu tố điều tiết thực vật đã được đặc trưng như as-1, LRE và ocs, G-box và các yếu tố giàu AT. Ngoài ra, 780BP đã được chứng minh là liên kết với các trình tự chồng lấp một yếu tố thụ thể hormone động vật có vú với ái lực lớn hơn yếu tố 780.
Từ khóa
#protein 780BP #gene 780 #T-DNA #bông cải #tương tác DNA-protein #methyl hóa #cạnh tranh động học #DNAse ITài liệu tham khảo
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