Novel functional assay for proteases and modulators
Tóm tắt
Proteases play a critical role in many cellular functions and have been an attractive therapeutic target due to their involvement in a number of disease processes. One prominent example is the secretases responsible for the generation of amyloid β peptide, which is believed to be central for the development of Alzheimer’s disease. It is therefore desirable to identify and characterize these proteases. We have developed a novel functional approach for identification of proteases and modulators by coupling the protease activity to caspase-mediated apoptosis. Here we show the proof of principle for this approach using β-secretase as an example. We provide data showing that
Therefore, this approach can be a useful tool in studies of proteolytic cleavage provided only that the protease recognition sequence is known.
Tài liệu tham khảo
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