Normal delivery induced stress alters glutathione peroxidase and TNF-α in elderly primigravidas mononuclear cells
Tóm tắt
To probe the glutathione peroxidase (GPx) activity coupled to ROS and TNF-α levels in younger versus elderly primigravidas. Monocytes were isolated from blood of young and elderly primigravidas after 12 hrs, 24 hrs and 14 days postpartum. Age-matched healthy female donors served as controls. Thereafter, the above monocytes were subjected to evaluation of GPx activity, levels of pro-inflammatory cytokine TNF-α and reactivity/specificity towards anti-TNF-α antibody. Suppression in GPx activity with simultaneous enhancement of TNF-α levels in monocytes was observed after 12 hrs and 24 hrs of spontaneous labor pain-induced maternal delivery through normal vaginal route. Conversely, an enhancement of GPx activity and decrease in TNF-α levels were observed on day 14 of postpartum, thereby indicating reversal of the impaired neutralizing mechanisms. The antioxidant network combating ROS was stronger in younger primigravidas. Capability of TNF-α and ROS suppression was somewhat lesser in elderly primigravidas even 14 days of postpartum via normal vaginal route. The capability of GPx activity enhancement with simultaneous suppression in ROS and TNF-α, decreases in elderly primigravidas. This may act as one of the important parameters leading to a variety of complications encountered by elderly primigravidas.
Tài liệu tham khảo
Islam N, Haqqi TM, Jepsen KJ, Kraay M, Welter JF, Goldberg VM, Malemud CJ. Hydrostatic pressure induces apoptosis in human chondrocytes from osteoarthritic cartilage through up-regulation of Tumor Necrosis Factor-alpha, inducible nitric oxide synthase, p53, c-myc, and bax-alpha and suppression of bcl-2. J Cell Biochem 2002; 87(3): 266–278.
Woller G, Brandt E, Mittelstädt J, Rybakowski C, Petersen F. Platelet factor 4/CXCL4-stimulated human monocytes induce apoptosis in endothelial cells by the release of oxygen radicals. J Leukocyte Biol 2008; 83: 936–945.
Stipek S, Mechurova A, Crkovska J, Zima T, Platenik J. Lipid peroxidation and superoxide dismutase activity in umbilical and maternal blood. Biochem Mol Biol Int 1995; 35: 705–711.
Tomlinson MK, Caruthers TJ, Whitty JE, Gonik B. Does delivery improve maternal condition in the respiratory-compromised gravida? Obstet Gynecol 1998; 91: 108–111.
Minnich ME, Brown M, Clark RB, Miller PC, Thompson DS. Oxygen desaturation in women in labor. J Reprod Med 1990; 35: 693–696.
Greiss FCJ, Anderson SG, King LC. Uterine pressure-flow relationships during early gestation. Am J Obstet Gynecol 1976; 126: 799–804.
Hasan, N, Yusuf, N, Toossi, Z and Islam, N. Suppression of Mycobacterium tuberculosis induced reactive oxygen species (ROS) and TNF-alpha mRNA expression in human monocytes by allicin. FEBS Letters 2006; 580: 2517–2522.
Mates JM, Segura JM, Perez-Gomez C, Rosado R, Olalla L, Blanca M, Sanchez-Jimenez FM. Antioxidant enzymatic activities in human blood cells after an allergic reaction to pollen or house dust mite. Blood Cells, Molecules and Diseases 1999; 25(7): 103–109.
Tasneem S, Islam N, Ali R. Crossreactivity of SLE Autoantibodies with 70 kDa Heat Shock Proteins of Mycobacterium tuberculosis. Microbiol Immunol 2001; 45(12): 841–846.
Islam N, Kanost RA, Teixeira-Johnson L, Hejal R, Aung H, Wilkinson RJ, Hirsch CS, Toossi Z. The role of cellular activation and tumor necrosis factor alpha (TNF-alpha) in the early expression of M. tuberculosis 85B mRNA in human alveolar macrophages. J Infect Dis 2004; 190: 341–351.
Staal FJ, Ela SW, Roederer M, Anderson MT, Herzenberg LA. Glutathione deficiency and human immunodeficiency virus infection. Lancet 1992; 339(8798): 909–912.
Deguchi K, Sasaki I, Ikeda K, Shimamura M, Urai Y, Tsukaguchi M, Touge T, Takeuchi H, Kuriyama S. Hyperventilation in autonomic failure. Int J Clin Pract 2007; 61(4): 695–696.