Non-covalent enzyme-polyelectrolyte complexes as self-buffered catalysts for use in low-water organic media
Tóm tắt
When free chymotrypsin is used to catalyse hydrolysis of N-acetyl tyrosine ethyl ester in 90% acetonitrile, the reaction rate soon falls because of the accumulation of the acidic product. If the enzyme is used in the form of a suspended complex with polyacrylic acid, the polyelectrolyte acts as an acid-base buffer to permit extended reaction.
Tài liệu tham khảo
Bell, G., Halling, P.J., Moore, B.D., Partridge, J. and Rees, D.G. (1995). Trends Biotechnol. 13, 468–473.
Blackwood, A.D., Curran, L.J., Moore, B.D. and Halling, P.J. (1994), Biochim. Biophys. Acta 1206, 161–165.
Carrea, G., Ottolina, G. and Riva, S. (1995). Trends Biotechnol. 13, 63–70.
Gill, I., Lopez-Fandino, R., Jorba, X. and Vulfson, E.N. (1996). Enzyme Microb. Technol. 18, 162–183.
Gladilin, A.K., Kudryashova, E.V., Vakurov, A.V., Izumrodov, V.A., Mozhaev, V.V. and Levashov, A.V. (1995). Biotechnol. Lett. 17, 1329–1334.
Kabanov, V.A. and Mustafaev, V.I. (1981). J. Vysokomolek. Soedineniy 23 A, 255–260.
Kamat, S.V., Beckman, E.J. and Russell, A.J. (1995). Crit. Rev. Biotech. 15, 41–71.
Kise, H. and Tomiuchi, Y. (1991). Biotechnol. Lett. 13, 317–322.
Kokufuta, E. (1994). In: Macromolecular Complexes in Chemistry and Biology, P.L. Dubin, J. Bock, R.M. Davies, D.N. Schultz and C. Thies, eds. pp 301–324, Berlin: Springer.
Kvittingen, L. (1994). Tetrahedron 50, 8253–8274.
Santaniello, E., Ferraboschi, P. and Grisenti, P. (1993). Enzyme Microb. Technol. 15, 367–382.
Schellenberger, V. and Jakubke, H.D. (1991). Angew. Chem. Int. Ed. 30, 1437–1449.
Sinisterra, J.V. and Alcantara, A.R. (1993). J. Mol. Catal. 84, 327–364.
Valivety, R.H., Rakels, J.L.L., Blanco, R.M., Johnston, G.A., Brown, L., Suckling, C.J. and Halling, P.J. (1990). Biotechnol. Lett. 12, 475–480.
Tramper, J., Vermue, M.H., Beeftink, H.H. and von Stockar, U. (1992) Biocatalysis in non-conventional media, Amsterdam: Elsevier.
Vermue, M.H. and Tramper, J. (1995). Pure Appl. Chem. 67, 345–373.
Xia, J. and Dubin, P.L. (1994). In: Macromolecular Complexes in Chemistry and Biology, P.L. Dubin, J. Bock, R.M. Davies, D.N. Schultz and C. Thies, eds. pp 247–270, Berlin: Springer.