Phát hiện nhạy cảm mới về vi mô ánh sáng của vàng keo trên các mẫu mỏng siêu mỏng bằng phép chiếu tương phản phản xạ

Springer Science and Business Media LLC - Tập 94 - Trang 61-71 - 1990
I. Cornelese-ten Velde1, F. A. Prins2
1Department of Cytochemistry and Cytometry, University of Leiden, Leiden, the Netherlands
2Department of Pathology, University of Leiden, Leiden, The Netherlands

Tóm tắt

Một phương pháp đơn giản sau khi nhúng để kết hợp giữa vi thể ánh sáng và điện tử được giới thiệu. Các loại kháng nguyên khác nhau trong thận của chuột và chuột cống bình thường cũng như trong các mô từ các trường hợp viêm thận do thí nghiệm gây ra đã được nhuộm sau khi nhúng bằng Lowicryl K4M bằng phương pháp vàng miễn dịch (bạc). Các hạt vàng (được tăng cường bằng bạc) đã được quan sát bằng vi thể ánh sáng, ví dụ như bằng vi thể sáng (BFM) và vi thể tương phản phản xạ (RCM), cũng như bằng vi thể điện tử. Tiềm năng của hình ảnh RCM trong lĩnh vực này đã được nghiên cứu, dẫn đến việc phát hiện thành công các hạt vàng keo (15 nm) hoặc các hạt vàng được tăng cường bằng bạc, trên các lát cắt siêu mỏng. Hơn nữa, một độ nhạy phát hiện gia tăng của RCM so với BFM cùng với một sự gia tăng trong độ nhạy của nhuộm miễn dịch bằng cách quan sát RCM đã được phát hiện. Các cách khác nhau để sử dụng RCM, riêng lẻ hoặc kết hợp với vi thể sáng hoặc vi thể tương phản pha để quan sát các phần nhựa khác nhau về độ dày, loại nhựa và nhuộm, đã được thảo luận.

Từ khóa

#Vi thể ánh sáng #vi thể điện tử #kháng nguyên #thận #viêm thận #hình ảnh #vàng keo #tương phản phản xạ

Tài liệu tham khảo

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