Neutralization of BDNF Attenuates the in vitro Protective Effects of Olfactory Ensheathing Cell-Conditioned Medium on Scratch-Insulted Retinal Ganglion Cells

Springer Science and Business Media LLC - Tập 31 - Trang 357-364 - 2010
Tao Wang1, Rui Cong2, Hao Yang1, Ming-Mei Wu1, Na Luo1, Fang Kuang1, Si-Wei You1
1Institute of Neurosciences, The Fourth Military Medical University, Xi’an, China
2Institute of Orthopedics at Xijing Hospital, The Fourth Military Medical University, Xi’an, China

Tóm tắt

Transplantation of olfactory ensheathing cells (OECs) becomes one of the promising strategies in restoring lost functions of injured central nervous system. Elevated level of expressed brain-derived neurotrophic factor (BDNF) was revealed in the previous studies to be related to the protective effects of OECs on injured cortical and brain stem neurons as well as retinal ganglion cells (RGCs), but no evidence has been obtained to demonstrate whether transplanted OECs protect injured central neurons directly by their secreted BDNF. In the present study, the effects of BDNF neutralization on the neuroprotection of adult OEC-conditioned medium (OEC-CM) on scratch-insulted RGCs were examined. The results showed that OEC-CM protected cultured RGCs from scratch insult, and neutralization of BDNF by BDNF neutralizing antibody attenuated such neuroprotection of the medium. It is thus concluded that neurotrophic factors including BDNF secreted by OECs can protect injured OECs in vitro and BDNF plays a major role in such a protection of OECs.

Tài liệu tham khảo

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