Network-based toxicogenomic approach to explore oral benzo(a)pyrene exposure effect on respiratory system

Molecular & Cellular Toxicology - Tập 18 - Trang 521-529 - 2022
Su Ji Kim1, Dong Yeop Shin1, Yujin Jang1, Sang Min Lee2, Cheol Min Lee3, Yeon Soon Ahn4, Young Rok Seo1
1Department of Life Science, Institute of Environmental Medicine for Green Chemistry, Dongguk University Biomedi Campus, Goyang-si, Republic of Korea
2Insilicogen Inc., Suwon-si, Republic of Korea
3Department of Chemical and Biological Engineering, College of Natural Science and Engineering, Seokyeong University, Seoul, Republic of Korea
4Department of Preventive Medicine, Institute of Occupational and Environmental Medicine, Wonju College of Medicine, Yonsei University, Wonju-si, Republic of Korea

Tóm tắt

Benzo(a)pyrene is known as a ubiquitous environmental pollutant. The general population can be exposed to benzo(a)pyrene through ambient air, tobacco smoke, water, and food. Genotoxicity and carcinogenicity are well-known adverse effects of benzo(a)pyrene exposure. However, few studies have focused on the effects of oral benzo(a)pyrene exposure on the respiratory system in terms of gene expression profiling. We explored the effect of benzo(a)pyrene on respiratory organs through bioinformatics analysis using public gene expression data. Key genes associated with respiratory toxicity were selected through network analysis and validated by Western blot and qRT-PCR. NRF2-mediated oxidative stress response and CREB signaling in neurons were identified as the main canonical pathways related to benzo(a)pyrene toxicity in the respiratory system. Potential biomarkers were validated in human lung cells. Our study suggested canonical pathways related to benzo(a)pyrene toxicity in the respiratory system and validated potential biomarkers in human lung cells.

Tài liệu tham khảo

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