Nested reverse transcriptase–polymerase chain reactions targeting the messenger RNA of icl2, hspx, and rRNAP1 genes to detect viable Mycobacterium tuberculosis directly from clinical specimens

Bloch, 1994, Nationwide survey of drug-resistant tuberculosis in the United States, JAMA, 271, 665, 10.1001/jama.1994.03510330043032 Li, 2010, Sputum Mycobacterium tuberculosis mRNA as a marker of bacteriologic clearance in response to antituberculosis therapy, J. Clin. Microbiol., 48, 46, 10.1128/JCM.01526-09 Hu, 2006, Deletion of the Mycobacterium tuberculosis alpha-crystallin-like hspx gene causes increased bacterial growth in vivo, Infect. Immun., 74, 861, 10.1128/IAI.74.2.861-868.2006 Kempsell, 1992, The nucleotide sequence of the promoter, 16s rRNA and spacer region of the ribosomal RNA operon of Mycobacterium tuberculosis and comparison with Mycobacterium leprae precursor rRNA, J. Gen. Microbiol., 138, 1717, 10.1099/00221287-138-8-1717 Therese, 2013, Diagnostic appraisal of simultaneous application of two nested PCRs targeting MPB64 gene and IS6110 region for rapid detection of M. tuberculosis genome in culture proven clinical specimens, Indian, J. Med. Microbiol., 31, 366, 10.4103/0255-0857.118887 Dietze, 2001, Safety and bactericidal activity of rifalazil in patients with pulmonary tuberculosis, Antimicrob. Agents Chemother., 45, 1972, 10.1128/AAC.45.7.1972-1976.2001 Muñoz-Elías, 2005, M. tuberculosis isocitrate lyases 1 and 2 are jointly required for in vivo growth and virulence, Nat. Med., 11, 638, 10.1038/nm1252 Bwanga, 2009, Direct susceptibility testing for multi drug resistant tuberculosis: a meta-analysis, BMC Infect. Dis., 9, 67, 10.1186/1471-2334-9-67