Nephroprotective effect of polyphenol-rich extract of Costus spicatus in cisplatin-induced nephrotoxicity in Wistar albino rats

3 Biotech - 2022
Amena Ali1, Abuzer Ali2, Wasim Ahmad3, Mohd Amir4, Kamran Ashraf5, Shadma Wahab6, Prawez Alam7, Abutahir8, Adil Ahamad9
1Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, Taif, Saudi Arabia
2Department of Pharmacognosy, College of Pharmacy, Taif University, Taif, Saudi Arabia
3Department of Pharmacy, Mohammed Al-Mana College for Medical Sciences, Dammam, Saudi Arabia
4Department of Natural Products and Alternative Medicine, College of Clinical Pharmacy, Imam Abdul Rahman Bin Faisal University, Dammam, Saudi Arabia
5Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, Bandar Puncak Alam, Malaysia
6Department of Pharmacognosy, College of Pharmacy, King Khalid University, Abha, Saudi Arabia
7Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia
8Hakikullah Chaudhary College of Pharmacy, Gonda, India
9Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India

Tóm tắt

Costus spicatus (spiked spiralflag ginger) is traditionally utilised for its advantages such as antidiabetic, antihyperlipidemic, diuretic, antimicrobial, and anticancer properties. However, there is no scientific evidence on the nephroprotective potential of this plant. Thus, this study tested the nephroprotective effect of the polyphenol-rich extract of Costus spicatus leaves (PCSL) using preclinical models, including the HeK cell line and Wistar albino rats against cisplatin-induced toxicity. It also determined the polyphenolic compounds using high-performance thin-layer chromatography (HPTLC). PCSL showed significant (p < 0.05) nephroprotective potential against cisplatin-induced nephrotoxicity in HeK cells. Moreover, in vivo studies revealed significant (p < 0.05) amelioration in serum biochemical markers and antioxidant enzymes against cisplatin-induced nephrotoxicity. PCSL significantly inhibited the level of inflammatory cytokines such as TNF-α, IL-6, and IL-1β. Moreover, PCSL restored the damage of the kidney tissues and ameliorated interstitial haemorrhage, congestion in capillaries, inflammatory cell infiltration, vacuolated cytoplasm, and tubular epithelial injury with widened Bowman's space. In addition, HPTLC analysis revealed that PCSL comprised polyphenolic compounds such as caffeic acid, quercetin, and ferulic acid. In conclusion, PCSL exerted nephroprotective potential by modulating the expression of inflammation, oxidative stress, and histological architecture of kidney tissues.

Từ khóa


Tài liệu tham khảo

Abd El-Rhman RH, El-Naga RN, Gad AM et al (2020) Dibenzazepine attenuates against cisplatin-induced nephrotoxicity in rats: involvement of NOTCH pathway. Front Pharmacol 11:567852

Afsar T, Razak S, Aldisi D, Shabbir M et al (2021) Acacia hydaspica R Parker ethyl-acetate extract abrogates cisplatin-induced nephrotoxicity by targeting ROS and inflammatory cytokines. Sci Rep 11:17248

Akca G, Eren H, Tumkaya L, Mercantepe T, Horsanali MO, Deveci E, Dil E, Yilmaz A (2018) The protective effect of astaxanthin against cisplatin-induced nephrotoxicity in rats. Biomed Pharmacother 100:575–582

Al-Naimi MS, Rasheed HA, Hussien NR, Al-Kuraishy HM, Al-Gareeb AI (2019) Nephrotoxicity: role and significance of renal biomarkers in the early detection of acute renal injury. J Adv Pharm Technol Res 10:95–99

Bami E, Ozakpınar OB, Ozdemir-Kumral ZN, Köroglu K, Ercan F, Cirakli Z, Sekerler T, Izzettin FV, Sancar M, Okuyan B (2017) Protective effect of ferulic acid on cisplatin induced nephrotoxicity in rats. Environ Toxicol Pharmacol 54:105–111

Batiha EG, Magdy Beshbishy A, Wasef GL, Elewa YH, Al-Sagan AA, El-Hack A, Mohamed E, Taha AE, Abd-Elhakim MY, Prasad Devkota H (2020) Chemical constituents and pharmacological activities of garlic A review. Nutrients 12(3):872

Chlopicka J, Pasko P, Gorinstein S, Jedryas A, Zagrodzki P (2012) Total phenolic and total flavonoid content, antioxidant activity and sensory evaluation of pseudocereal breads. LWT-Food Science and Technology 46:548–555

Daenen K, Andries A, Mekahli D, Van Schepdael A, Jouret F, Bammens B (2019) Oxidative stress in chronic kidney disease. Pediatr Nephrol 34:975–991

Dasari S, Tchounwou BP (2014) Cisplatin in cancer therapy: Molecular mechanisms of action. European Journal of Pharmacology. Eur J Pharmacol 740:364–378

Dixit V, Irshad S, Singh H, Agnihotri P, Husain T, Khatoon S (2017) High-performance thin-layer chromatographic determination of three therapeutic phenolic components in Leucas species. JPC-J Planar Chromat 30:25–31

Elsawy H, Alzahrani AM, Alfwuaires M, Abdel-Moneim AM, Khalil M (2021) Nephroprotective effect of naringin in methotrexate induced renal toxicity in male rats. Biomed Pharmacother 143:112180

Ewees MG, Messiha BA, Abo-Saif AA, Bayoumi A, Abdel-Bakky MS (2018) Erratum: interference with coagulation cascade as a novel approach to counteract cisplatin-induced acute tubular necrosis; an experimental study in rats. Front Pharmacol 9:1155

Fang CY, Lou DY, Zhou LQ, Wang JC, Yang B, He QJ, Wang JJ, Weng QJ (2021) Natural products: potential treatments for cisplatin-induced nephrotoxicity. Acta Pharmacol Sin 9:1–9

Farooqui Z, Ahmed F, Rizwan S, Shahid F, Khan AA, Khan F (2017) Protective effect of Nigella sativa oil on cisplatin induced nephrotoxicity and oxidative damage in rat kidney. Biomed Pharmacother 85:7–15

Fierascu RC, Fierascu I, Ortan A, Georgiev MI, Sieniawska E (2020) Innovative approaches for recovery of phytoconstituents from medicinal/aromatic plants and biotechnological production. Molecules 25:309

Humanes B, Camaño S, Lara JM, Sabbisetti V, González-Nicolás MÁ, Bonventre JV, Tejedor A, Lázaro A (2017) Cisplatin-induced renal inflammation is ameliorated by cilastatin nephroprotection. Nephrol Dial Transplant 32:1645–1655

Ighodaro OM, Akinloye OA (2018) First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria J Med 54:287–293

Ingale KG, Thakurdesai PA, Vyawahare NS (2013) Protective effect of Hygrophila spinosa against cisplatin induced nephrotoxicity in rats. Indian J Pharmacol 45:232–236

Kpemissi M, Eklu-Gadegbeku K, Veerapur VP, Negru M, Taulescu M, Chandramohan V, Hiriyan J, Banakar SM, Thimmaiah NV, Suhas DS, Puneeth TA (2019) Nephroprotective activity of Combretum micranthum G Don in cisplatin induced nephrotoxicity in rats: In-vitro, in-vivo and in-silico experiments. Biomed Pharmacother 116:108961

Kumar M, Dahiya V, Kasala ER, Bodduluru LN, Lahkar M (2017) The renoprotective activity of hesperetin in cisplatin induced nephrotoxicity in rats: molecular and biochemical evidence. Biomed Pharmacother 89:1207–1215

Lin L, Zheng J, Zhu W, Jia N (2015) Nephroprotective effect of gelsemine against cisplatin-induced toxicity is mediated via attenuation of oxidative stress. Cell Biochem Biophys 71:535–541

Lv JC, Zhang LX (2019) Prevalence and disease burden of chronic kidney disease. Adv Exp Med Biol 1165:3–15

Miller RP, Tadagavadi RK, Ramesh G, Reeves WB (2010) Mechanisms of cisplatin nephrotoxicity. Toxins 2:2490–2518

Mohamed ME, Abduldaium YS, Younis NS (2020) Ameliorative effect of linalool in cisplatin-induced nephrotoxicity: The role of HMGB1/TLR4/NF-κB and NRF2/HO1 pathways. Biomolecules 10:1–19

Moreno KG, Junior AG, Dos Santos AC, Palozi RA, Guarnier LP, Marques AA, Romão PV, Lorençone BR, Cassemiro NS, Silva DB, Tirloni CA (2021) Nephroprotective and antilithiatic activities of Costus spicatus Sw: Ethnopharmacological investigation of a species from the Dourados region, Mato Grosso do Sul State. Brazil J Ethnopharmacol 266:113409

Neelima S, Dwarakanadha Reddy P, Kothapalli Bannoth CS (2020) Nephroprotective activity of Annona Squamosa leaves against paracetamol-induced nephrotoxicity in rats: in vitro and in vivo experiments. Future J Pharm Sci 6:1–8

Özen S, Akyol Ö, Iraz M, Söğüt S, Özuğurlu F, Özyurt H, Odacı E, Yıldırım Z (2004) Role of caffeic acid phenethyl ester, an active component of propolis, against cisplatin-induced nephrotoxicity in rats. J Appl Toxicol 24:27–35

Peres LA, Cunha Júnior AD (2013) Acute nephrotoxicity of cisplatin: molecular mechanisms. J Bras Nefrol 35(4):332–340

Pizzorno J (2015) The kidney dysfunction epidemic, part 1: causes. Integrative Med: Clin J 14:8

Rezaee-Khorasany A, Razavi BM, Taghiabadi E, Yazdi AT, Hosseinzadeh H (2020) Effect of crocin, an active saffron constituent, on ethanol toxicity in the rat: Histopathological and biochemical studies. Iran J Basic Med Sci 23:51–62

Sanchez-Gonzalez PD, Lopez-Hernandez FJ, Perez-Barriocanal F, Morales AI, Lopez-Novoa JM (2011) Quercetin reduces cisplatin nephrotoxicity in rats without compromising its anti-tumour activity. Nephrol Dial Transplant 26:3484–3495

Shediwah FM, Naji KM, Gumaih HS, Alhadi FA, Al-Hammami AL, D’Souza MR (2019) Antioxidant and antihyperlipidemic activity of Costus speciosus against atherogenic diet-induced hyperlipidemia in rabbits. J Integr Med 17:181–191

Singh HP, Singh TG, Singh R (2020) Sinapic acid attenuates cisplatin-induced nephrotoxicity through peroxisome proliferator-activated receptor gamma agonism in rats. J Pharm Bioallied Sci 12:146–154

Sultana S, Verma K, Khan R (2012) Nephroprotective efficacy of chrysin against cisplatin-induced toxicity via attenuation of oxidative stress. J Pharm Pharmacol 64:872–881

Wang TE, Liu HT, Lai YH, Jan TR, Nomura N, Chang HW, Chou CC, Lee YJ, Tsai PS (2018) Honokiol, a polyphenol natural compound, attenuates cisplatin-induced acute cytotoxicity in renal epithelial cells through cellular oxidative stress and cytoskeleton modulations. Front Pharmacol 9:357

Xavier S, Haneefa SM, Anand DR (2017) Antioxidant and nephroprotective activities of the extract and fractions of Homonoia riparia Lour. Pharmacog Mag 13:25–30

Thông tin
Thông tin xuất bản

3 Biotech

Thông tin tác giả