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Biểu hiện yếu tố phiên mã NAC, nồng độ axit amin và sự phát triển của các giống lúa tinh hoa dưới áp lực muối
Tóm tắt
Các yếu tố phiên mã NAC (TF) đóng vai trò quan trọng trong việc điều chỉnh khả năng chịu stress thẩm thấu ở thực vật. Chúng tôi đã kiểm tra sự biểu hiện của 57 gen NAC trong sự hiện diện của NaCl trong lá non của hai giống lúa tinh hoa, Cotaxtla và Tres Ríos, mà thể hiện các phản ứng trái ngược đối với độ mặn ở mức độ sinh hóa và sinh lý. Bằng cách sử dụng qRT-PCR, sự biểu hiện của 41 trong số 57 gen NAC đã được xác nhận, trong đó 23 gen cho thấy sự điều chỉnh bởi NaCl. Chúng tôi đã xác định hai gen NAC (Os02g56600 và Os12g07790) được kích thích trong Cotaxtla, nhưng bị ức chế trong Tres Ríos khi cây được tiếp xúc với dung dịch dinh dưỡng có 100 mM NaCl. Cả hai giống lúa tinh hoa đều cho thấy cây được xử lý có nồng độ axit amin tổng cộng và proline cao hơn so với đối chứng; trong tất cả các trường hợp, cây Cotaxtla tích lũy nhiều axit amin tự do và proline hơn so với cây Tres Ríos. Hơn nữa, sự phát triển chồi bị ảnh hưởng nhiều hơn ở cả hai giống, trong khi chiều dài rễ không bị giảm ở cây được xử lý so với đối chứng. Chúng tôi kết luận rằng cả hai giống lúa tinh hoa này thể hiện các mô hình biểu hiện khác nhau của các yếu tố phiên mã NAC cũng như các phản ứng sinh hóa và sinh lý đối với stress muối, dẫn đến hiệu suất tốt hơn của cây Cotaxtla so với cây Tres Ríos dưới các điều kiện thí nghiệm của chúng tôi.
Từ khóa
#NAC transcription factors #rice cultivars #osmotic stress #NaCl #qRT-PCR #amino acids #proline #salinity toleranceTài liệu tham khảo
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